Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel
Abstract Background Current methods to classify local and imported malaria infections depend primarily on patient travel history, which can have limited accuracy. Genotyping has been investigated as a complementary approach to track the spread of malaria and identify the origin of imported infection...
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ftdoajarticles:oai:doaj.org/article:a5a4710fcab44fc1ae7af871f8117357 2023-05-15T15:13:32+02:00 Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel Yaobao Liu Sofonias K. Tessema Maxwell Murphy Sui Xu Alanna Schwartz Weiming Wang Yuanyuan Cao Feng Lu Jianxia Tang Yaping Gu Guoding Zhu Huayun Zhou Qi Gao Rui Huang Jun Cao Bryan Greenhouse 2020-07-01T00:00:00Z https://doi.org/10.1186/s12936-020-03316-3 https://doaj.org/article/a5a4710fcab44fc1ae7af871f8117357 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03316-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03316-3 1475-2875 https://doaj.org/article/a5a4710fcab44fc1ae7af871f8117357 Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) Malaria Imported malaria Local transmission Jiangsu China Microsatellite genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03316-3 2022-12-31T13:31:21Z Abstract Background Current methods to classify local and imported malaria infections depend primarily on patient travel history, which can have limited accuracy. Genotyping has been investigated as a complementary approach to track the spread of malaria and identify the origin of imported infections. Methods An extended panel of 26 microsatellites (16 new microsatellites) for Plasmodium falciparum was evaluated in 602 imported infections from 26 sub-Saharan African countries to the Jiangsu Province of People’s Republic of China. The potential of the 26 microsatellite markers to assign imported parasites to their geographic origin was assessed using a Bayesian method with Markov Chain Monte Carlo (MCMC) as implemented in the program Smoothed and Continuous Assignments (SCAT) with a modification to incorporate haploid genotype data. Results The newly designed microsatellites were polymorphic and are not in linkage disequilibrium with the existing microsatellites, supporting previous findings of high rate of recombination in sub-Saharan Africa. Consistent with epidemiology inferred from patients’ travel history, no evidence for local transmission was found; nearly all genetically related infections were identified in people who travelled to the same country near the same time. The smoothing assignment method assigned imported cases to their likely geographic origin with an accuracy (Angola: 59%; Nigeria: 51%; Equatorial Guinea: 40%) higher than would be achieved at random, reaching statistical significance for Angola and Equatorial Guinea. Conclusions Genotyping using an extended microsatellite panel is valuable for malaria case classification and programme evaluation in an elimination setting. A Bayesian method for assigning geographic origin of mammals based on genetic data was adapted for malaria and showed potential for identification of the origin of imported infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Imported malaria Local transmission Jiangsu China Microsatellite genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Imported malaria Local transmission Jiangsu China Microsatellite genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yaobao Liu Sofonias K. Tessema Maxwell Murphy Sui Xu Alanna Schwartz Weiming Wang Yuanyuan Cao Feng Lu Jianxia Tang Yaping Gu Guoding Zhu Huayun Zhou Qi Gao Rui Huang Jun Cao Bryan Greenhouse Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
topic_facet |
Malaria Imported malaria Local transmission Jiangsu China Microsatellite genotyping Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Current methods to classify local and imported malaria infections depend primarily on patient travel history, which can have limited accuracy. Genotyping has been investigated as a complementary approach to track the spread of malaria and identify the origin of imported infections. Methods An extended panel of 26 microsatellites (16 new microsatellites) for Plasmodium falciparum was evaluated in 602 imported infections from 26 sub-Saharan African countries to the Jiangsu Province of People’s Republic of China. The potential of the 26 microsatellite markers to assign imported parasites to their geographic origin was assessed using a Bayesian method with Markov Chain Monte Carlo (MCMC) as implemented in the program Smoothed and Continuous Assignments (SCAT) with a modification to incorporate haploid genotype data. Results The newly designed microsatellites were polymorphic and are not in linkage disequilibrium with the existing microsatellites, supporting previous findings of high rate of recombination in sub-Saharan Africa. Consistent with epidemiology inferred from patients’ travel history, no evidence for local transmission was found; nearly all genetically related infections were identified in people who travelled to the same country near the same time. The smoothing assignment method assigned imported cases to their likely geographic origin with an accuracy (Angola: 59%; Nigeria: 51%; Equatorial Guinea: 40%) higher than would be achieved at random, reaching statistical significance for Angola and Equatorial Guinea. Conclusions Genotyping using an extended microsatellite panel is valuable for malaria case classification and programme evaluation in an elimination setting. A Bayesian method for assigning geographic origin of mammals based on genetic data was adapted for malaria and showed potential for identification of the origin of imported infections. |
format |
Article in Journal/Newspaper |
author |
Yaobao Liu Sofonias K. Tessema Maxwell Murphy Sui Xu Alanna Schwartz Weiming Wang Yuanyuan Cao Feng Lu Jianxia Tang Yaping Gu Guoding Zhu Huayun Zhou Qi Gao Rui Huang Jun Cao Bryan Greenhouse |
author_facet |
Yaobao Liu Sofonias K. Tessema Maxwell Murphy Sui Xu Alanna Schwartz Weiming Wang Yuanyuan Cao Feng Lu Jianxia Tang Yaping Gu Guoding Zhu Huayun Zhou Qi Gao Rui Huang Jun Cao Bryan Greenhouse |
author_sort |
Yaobao Liu |
title |
Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
title_short |
Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
title_full |
Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
title_fullStr |
Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
title_full_unstemmed |
Confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to China using microsatellite panel |
title_sort |
confirmation of the absence of local transmission and geographic assignment of imported falciparum malaria cases to china using microsatellite panel |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03316-3 https://doaj.org/article/a5a4710fcab44fc1ae7af871f8117357 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-11 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03316-3 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03316-3 1475-2875 https://doaj.org/article/a5a4710fcab44fc1ae7af871f8117357 |
op_doi |
https://doi.org/10.1186/s12936-020-03316-3 |
container_title |
Malaria Journal |
container_volume |
19 |
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1 |
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1766344074624761856 |