Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E
Objective: To prove the molecular mechanisms of Mahkota Dewa (Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression. Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extrac...
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2017.01.001 https://doaj.org/article/a55e4d327c2b4d60bbae1b1e8edfb81a |
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ftdoajarticles:oai:doaj.org/article:a55e4d327c2b4d60bbae1b1e8edfb81a 2023-05-15T15:06:55+02:00 Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E Nugroho Trilaksana Ignatius Riwanto Raymond Rubianto Tjandrawinata Reki Winarto 2017-04-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.01.001 https://doaj.org/article/a55e4d327c2b4d60bbae1b1e8edfb81a EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116306190 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.001 https://doaj.org/article/a55e4d327c2b4d60bbae1b1e8edfb81a Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 4, Pp 280-287 (2017) Phaleria macrocarpa Mahkota Dewa DLBS1425 Retinoblastoma Cyclin E Cell cycle Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.01.001 2022-12-31T15:52:35Z Objective: To prove the molecular mechanisms of Mahkota Dewa (Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression. Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa (DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D, cyclin E, and E2F. Results: DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line. Inhibition of proliferation was shown by suppression of cell cycle progression. DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dose-dependent manner without affecting p53–p21 pathway. In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway. Conclusions: Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 4 280 287 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Phaleria macrocarpa Mahkota Dewa DLBS1425 Retinoblastoma Cyclin E Cell cycle Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Phaleria macrocarpa Mahkota Dewa DLBS1425 Retinoblastoma Cyclin E Cell cycle Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Nugroho Trilaksana Ignatius Riwanto Raymond Rubianto Tjandrawinata Reki Winarto Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
topic_facet |
Phaleria macrocarpa Mahkota Dewa DLBS1425 Retinoblastoma Cyclin E Cell cycle Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To prove the molecular mechanisms of Mahkota Dewa (Phaleria macrocarpa) in suppressing proliferation of human retinoblastoma cells through suppression of cell cycle's gene-regulators expression. Methods: In this study, the molecular mechanism of anti-tumor effect of fractioned extract of Phaleria macrocarpa (DLBS1425) in human retinoblastoma cells Y-79 was investigated by measuring the tumor cells viability, the assessment of population profiles of tumor cells in the cell cycle, and the mRNA concentration of p16, p21, p53, cyclin D, cyclin E, and E2F. Results: DLBS1425 showed an inhibition effects towards proliferation of Y-79 cell line. Inhibition of proliferation was shown by suppression of cell cycle progression. DLBS1425 downregulated cyclin E, a G1 phase regulator gene of cell cycle, in dose-dependent manner without affecting p53–p21 pathway. In the other word, DLBS1425 inhibits cell proliferation through suppression of cyclin E independently towards conventional proliferation pathway. Conclusions: Our results suggest that DLBS1425 is a potential anticancer agent which targets genes involved in cell proliferation in human retinoblastoma cells which make it pharmacologically ideal for the prevention and/or treatment of retinoblastoma cancer. |
format |
Article in Journal/Newspaper |
author |
Nugroho Trilaksana Ignatius Riwanto Raymond Rubianto Tjandrawinata Reki Winarto |
author_facet |
Nugroho Trilaksana Ignatius Riwanto Raymond Rubianto Tjandrawinata Reki Winarto |
author_sort |
Nugroho Trilaksana |
title |
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
title_short |
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
title_full |
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
title_fullStr |
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
title_full_unstemmed |
Inhibition of Mahkota Dewa (Phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells Y-79 through suppression of mRNA level of cyclin E |
title_sort |
inhibition of mahkota dewa (phaleria macrocarpa) bioactive fraction on proliferation of human retinoblastoma tumor cells y-79 through suppression of mrna level of cyclin e |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.01.001 https://doaj.org/article/a55e4d327c2b4d60bbae1b1e8edfb81a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 4, Pp 280-287 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116306190 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.001 https://doaj.org/article/a55e4d327c2b4d60bbae1b1e8edfb81a |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.01.001 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
4 |
container_start_page |
280 |
op_container_end_page |
287 |
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1766338510206271488 |