A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat C...
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ftdoajarticles:oai:doaj.org/article:a5519763f5ed4c21871195685d88c14a 2024-02-11T10:03:22+01:00 A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases Rui-fang Zheng Kaderyea Kader Di-wei Liu Wen-ling Su Lei Xu Yuan-yuan Jin Jian-guo Xing 2024-01-01T00:00:00Z https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/article/a5519763f5ed4c21871195685d88c14a EN eng BMC https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/toc/2662-7671 doi:10.1186/s12906-023-04316-x 2662-7671 https://doaj.org/article/a5519763f5ed4c21871195685d88c14a BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-16 (2024) Total Flavonoids from Dracocephalum Moldavica L Cardiovascular disease Network pharmacology Pharmacological evaluation Recombinant NADPH oxidase 4 Other systems of medicine RZ201-999 article 2024 ftdoajarticles https://doi.org/10.1186/s12906-023-04316-x 2024-01-14T01:52:14Z Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat CVD has not been systematically determined. Materials and methods The active compounds in DML were screened by literature mining and pharmacokinetic analysis. Cytoscape software was used to construct the target-disease interaction network of DML in the treatment of CVD. Gene ontology and signalling pathway enrichment analyses were performed. The key target pathway network of DML compounds was constructed and verified by pharmacological experiments in vitro. A hydrogen glucose deprivation/reoxygenation model was established in H9c2 cells using hypoxia and glucose deprivation for 9 h combined with reoxygenation for 2 h. The model simulated myocardial ischaemic reperfusion injury to investigate the effects of total flavonoids of Cymbidium on cell viability, myocardial injury markers, oxidative stress levels, and reactive oxygen radical levels. Western blot analysis was used to examine NOX-4, Bcl-2/Bax, and PGC-1α protein expression. Results Twenty-seven active components were screened, and 59 potential drug targets for the treatment of CVD were obtained. Through the compound-target interaction network and the target-disease interaction network, the key targets and key signalling pathways, such as NOX-4, Bcl-2/Bax and PGC-1α, were obtained. TFDM significantly decreased LDH and MDA levels and the production of ROS and increased SOD activity levels in the context of OGD/R injury. Further studies indicated that NOX-4 and Bax protein levels and the p-P38 MAPK/P38 MAPK andp-Erk1/2/Erk1/2 ratios were suppressed by TFDM. The protein expression of Bcl-2 and PGC-1α was increased by TFDM. Conclusions Our results showed that DML had multicomponent, multitarget and multichannel characteristics in the treatment of CVD. The mechanism ... Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles BMC Complementary Medicine and Therapies 24 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Total Flavonoids from Dracocephalum Moldavica L Cardiovascular disease Network pharmacology Pharmacological evaluation Recombinant NADPH oxidase 4 Other systems of medicine RZ201-999 |
spellingShingle |
Total Flavonoids from Dracocephalum Moldavica L Cardiovascular disease Network pharmacology Pharmacological evaluation Recombinant NADPH oxidase 4 Other systems of medicine RZ201-999 Rui-fang Zheng Kaderyea Kader Di-wei Liu Wen-ling Su Lei Xu Yuan-yuan Jin Jian-guo Xing A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
topic_facet |
Total Flavonoids from Dracocephalum Moldavica L Cardiovascular disease Network pharmacology Pharmacological evaluation Recombinant NADPH oxidase 4 Other systems of medicine RZ201-999 |
description |
Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat CVD has not been systematically determined. Materials and methods The active compounds in DML were screened by literature mining and pharmacokinetic analysis. Cytoscape software was used to construct the target-disease interaction network of DML in the treatment of CVD. Gene ontology and signalling pathway enrichment analyses were performed. The key target pathway network of DML compounds was constructed and verified by pharmacological experiments in vitro. A hydrogen glucose deprivation/reoxygenation model was established in H9c2 cells using hypoxia and glucose deprivation for 9 h combined with reoxygenation for 2 h. The model simulated myocardial ischaemic reperfusion injury to investigate the effects of total flavonoids of Cymbidium on cell viability, myocardial injury markers, oxidative stress levels, and reactive oxygen radical levels. Western blot analysis was used to examine NOX-4, Bcl-2/Bax, and PGC-1α protein expression. Results Twenty-seven active components were screened, and 59 potential drug targets for the treatment of CVD were obtained. Through the compound-target interaction network and the target-disease interaction network, the key targets and key signalling pathways, such as NOX-4, Bcl-2/Bax and PGC-1α, were obtained. TFDM significantly decreased LDH and MDA levels and the production of ROS and increased SOD activity levels in the context of OGD/R injury. Further studies indicated that NOX-4 and Bax protein levels and the p-P38 MAPK/P38 MAPK andp-Erk1/2/Erk1/2 ratios were suppressed by TFDM. The protein expression of Bcl-2 and PGC-1α was increased by TFDM. Conclusions Our results showed that DML had multicomponent, multitarget and multichannel characteristics in the treatment of CVD. The mechanism ... |
format |
Article in Journal/Newspaper |
author |
Rui-fang Zheng Kaderyea Kader Di-wei Liu Wen-ling Su Lei Xu Yuan-yuan Jin Jian-guo Xing |
author_facet |
Rui-fang Zheng Kaderyea Kader Di-wei Liu Wen-ling Su Lei Xu Yuan-yuan Jin Jian-guo Xing |
author_sort |
Rui-fang Zheng |
title |
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
title_short |
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
title_full |
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
title_fullStr |
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
title_full_unstemmed |
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases |
title_sort |
network pharmacology approach to decipher the mechanism of total flavonoids from dracocephalum moldavica l. in the treatment of cardiovascular diseases |
publisher |
BMC |
publishDate |
2024 |
url |
https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/article/a5519763f5ed4c21871195685d88c14a |
genre |
DML |
genre_facet |
DML |
op_source |
BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-16 (2024) |
op_relation |
https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/toc/2662-7671 doi:10.1186/s12906-023-04316-x 2662-7671 https://doaj.org/article/a5519763f5ed4c21871195685d88c14a |
op_doi |
https://doi.org/10.1186/s12906-023-04316-x |
container_title |
BMC Complementary Medicine and Therapies |
container_volume |
24 |
container_issue |
1 |
_version_ |
1790599580531818496 |