A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases

Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat C...

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Published in:BMC Complementary Medicine and Therapies
Main Authors: Rui-fang Zheng, Kaderyea Kader, Di-wei Liu, Wen-ling Su, Lei Xu, Yuan-yuan Jin, Jian-guo Xing
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2024
Subjects:
DML
Online Access:https://doi.org/10.1186/s12906-023-04316-x
https://doaj.org/article/a5519763f5ed4c21871195685d88c14a
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spelling ftdoajarticles:oai:doaj.org/article:a5519763f5ed4c21871195685d88c14a 2024-02-11T10:03:22+01:00 A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases Rui-fang Zheng Kaderyea Kader Di-wei Liu Wen-ling Su Lei Xu Yuan-yuan Jin Jian-guo Xing 2024-01-01T00:00:00Z https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/article/a5519763f5ed4c21871195685d88c14a EN eng BMC https://doi.org/10.1186/s12906-023-04316-x https://doaj.org/toc/2662-7671 doi:10.1186/s12906-023-04316-x 2662-7671 https://doaj.org/article/a5519763f5ed4c21871195685d88c14a BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-16 (2024) Total Flavonoids from Dracocephalum Moldavica L Cardiovascular disease Network pharmacology Pharmacological evaluation Recombinant NADPH oxidase 4 Other systems of medicine RZ201-999 article 2024 ftdoajarticles https://doi.org/10.1186/s12906-023-04316-x 2024-01-14T01:52:14Z Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat CVD has not been systematically determined. Materials and methods The active compounds in DML were screened by literature mining and pharmacokinetic analysis. Cytoscape software was used to construct the target-disease interaction network of DML in the treatment of CVD. Gene ontology and signalling pathway enrichment analyses were performed. The key target pathway network of DML compounds was constructed and verified by pharmacological experiments in vitro. A hydrogen glucose deprivation/reoxygenation model was established in H9c2 cells using hypoxia and glucose deprivation for 9 h combined with reoxygenation for 2 h. The model simulated myocardial ischaemic reperfusion injury to investigate the effects of total flavonoids of Cymbidium on cell viability, myocardial injury markers, oxidative stress levels, and reactive oxygen radical levels. Western blot analysis was used to examine NOX-4, Bcl-2/Bax, and PGC-1α protein expression. Results Twenty-seven active components were screened, and 59 potential drug targets for the treatment of CVD were obtained. Through the compound-target interaction network and the target-disease interaction network, the key targets and key signalling pathways, such as NOX-4, Bcl-2/Bax and PGC-1α, were obtained. TFDM significantly decreased LDH and MDA levels and the production of ROS and increased SOD activity levels in the context of OGD/R injury. Further studies indicated that NOX-4 and Bax protein levels and the p-P38 MAPK/P38 MAPK andp-Erk1/2/Erk1/2 ratios were suppressed by TFDM. The protein expression of Bcl-2 and PGC-1α was increased by TFDM. Conclusions Our results showed that DML had multicomponent, multitarget and multichannel characteristics in the treatment of CVD. The mechanism ... Article in Journal/Newspaper DML Directory of Open Access Journals: DOAJ Articles BMC Complementary Medicine and Therapies 24 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Total Flavonoids from Dracocephalum Moldavica L
Cardiovascular disease
Network pharmacology
Pharmacological evaluation
Recombinant NADPH oxidase 4
Other systems of medicine
RZ201-999
spellingShingle Total Flavonoids from Dracocephalum Moldavica L
Cardiovascular disease
Network pharmacology
Pharmacological evaluation
Recombinant NADPH oxidase 4
Other systems of medicine
RZ201-999
Rui-fang Zheng
Kaderyea Kader
Di-wei Liu
Wen-ling Su
Lei Xu
Yuan-yuan Jin
Jian-guo Xing
A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
topic_facet Total Flavonoids from Dracocephalum Moldavica L
Cardiovascular disease
Network pharmacology
Pharmacological evaluation
Recombinant NADPH oxidase 4
Other systems of medicine
RZ201-999
description Abstract Aim of the study Cardiovascular disease (CVD) seriously endangers human health and is characterized by high mortality and disability. The effectiveness of Dracocephalum moldavica L. in the treatment of CVD has been proven by clinical practice. However, the mechanism by which DML can treat CVD has not been systematically determined. Materials and methods The active compounds in DML were screened by literature mining and pharmacokinetic analysis. Cytoscape software was used to construct the target-disease interaction network of DML in the treatment of CVD. Gene ontology and signalling pathway enrichment analyses were performed. The key target pathway network of DML compounds was constructed and verified by pharmacological experiments in vitro. A hydrogen glucose deprivation/reoxygenation model was established in H9c2 cells using hypoxia and glucose deprivation for 9 h combined with reoxygenation for 2 h. The model simulated myocardial ischaemic reperfusion injury to investigate the effects of total flavonoids of Cymbidium on cell viability, myocardial injury markers, oxidative stress levels, and reactive oxygen radical levels. Western blot analysis was used to examine NOX-4, Bcl-2/Bax, and PGC-1α protein expression. Results Twenty-seven active components were screened, and 59 potential drug targets for the treatment of CVD were obtained. Through the compound-target interaction network and the target-disease interaction network, the key targets and key signalling pathways, such as NOX-4, Bcl-2/Bax and PGC-1α, were obtained. TFDM significantly decreased LDH and MDA levels and the production of ROS and increased SOD activity levels in the context of OGD/R injury. Further studies indicated that NOX-4 and Bax protein levels and the p-P38 MAPK/P38 MAPK andp-Erk1/2/Erk1/2 ratios were suppressed by TFDM. The protein expression of Bcl-2 and PGC-1α was increased by TFDM. Conclusions Our results showed that DML had multicomponent, multitarget and multichannel characteristics in the treatment of CVD. The mechanism ...
format Article in Journal/Newspaper
author Rui-fang Zheng
Kaderyea Kader
Di-wei Liu
Wen-ling Su
Lei Xu
Yuan-yuan Jin
Jian-guo Xing
author_facet Rui-fang Zheng
Kaderyea Kader
Di-wei Liu
Wen-ling Su
Lei Xu
Yuan-yuan Jin
Jian-guo Xing
author_sort Rui-fang Zheng
title A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
title_short A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
title_full A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
title_fullStr A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
title_full_unstemmed A network pharmacology approach to decipher the mechanism of total flavonoids from Dracocephalum Moldavica L. in the treatment of cardiovascular diseases
title_sort network pharmacology approach to decipher the mechanism of total flavonoids from dracocephalum moldavica l. in the treatment of cardiovascular diseases
publisher BMC
publishDate 2024
url https://doi.org/10.1186/s12906-023-04316-x
https://doaj.org/article/a5519763f5ed4c21871195685d88c14a
genre DML
genre_facet DML
op_source BMC Complementary Medicine and Therapies, Vol 24, Iss 1, Pp 1-16 (2024)
op_relation https://doi.org/10.1186/s12906-023-04316-x
https://doaj.org/toc/2662-7671
doi:10.1186/s12906-023-04316-x
2662-7671
https://doaj.org/article/a5519763f5ed4c21871195685d88c14a
op_doi https://doi.org/10.1186/s12906-023-04316-x
container_title BMC Complementary Medicine and Therapies
container_volume 24
container_issue 1
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