Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
The neuropeptides involved in the regulation of reproduction in the Pacific oyster ( Crassostrea gigas ) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples correspondin...
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2021
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ftdoajarticles:oai:doaj.org/article:a541bd386a744488bf7b49532911d21b 2023-05-15T15:57:58+02:00 Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel 2021-08-01T00:00:00Z https://doi.org/10.3390/md19080452 https://doaj.org/article/a541bd386a744488bf7b49532911d21b EN eng MDPI AG https://www.mdpi.com/1660-3397/19/8/452 https://doaj.org/toc/1660-3397 doi:10.3390/md19080452 1660-3397 https://doaj.org/article/a541bd386a744488bf7b49532911d21b Marine Drugs, Vol 19, Iss 452, p 452 (2021) nervous system transcriptome neuropeptides reproduction Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19080452 2022-12-30T20:28:11Z The neuropeptides involved in the regulation of reproduction in the Pacific oyster ( Crassostrea gigas ) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Marine Drugs 19 8 452 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
nervous system transcriptome neuropeptides reproduction Biology (General) QH301-705.5 |
spellingShingle |
nervous system transcriptome neuropeptides reproduction Biology (General) QH301-705.5 Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
topic_facet |
nervous system transcriptome neuropeptides reproduction Biology (General) QH301-705.5 |
description |
The neuropeptides involved in the regulation of reproduction in the Pacific oyster ( Crassostrea gigas ) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. |
format |
Article in Journal/Newspaper |
author |
Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel |
author_facet |
Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel |
author_sort |
Emilie Réalis-Doyelle |
title |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_short |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_fullStr |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full_unstemmed |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_sort |
transcriptome profiling of the pacific oyster crassostrea gigas visceral ganglia over a reproduction cycle identifies novel regulatory peptides |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19080452 https://doaj.org/article/a541bd386a744488bf7b49532911d21b |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs, Vol 19, Iss 452, p 452 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/8/452 https://doaj.org/toc/1660-3397 doi:10.3390/md19080452 1660-3397 https://doaj.org/article/a541bd386a744488bf7b49532911d21b |
op_doi |
https://doi.org/10.3390/md19080452 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
452 |
_version_ |
1766393692501835776 |