Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018.
Background Shifts have occurred in the epidemiological characteristics of Japanese encephalitis (JE), extending from the molecular level to the population level. The aim of this study was to investigate the seroprevalence of JE neutralizing antibodies in healthy populations from different age groups...
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ftdoajarticles:oai:doaj.org/article:a52c0dd8f58e402da2e8439c08b6fbfb 2023-05-15T15:17:53+02:00 Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. Xuan Deng Ju-Ying Yan Han-Qing He Rui Yan Yi Sun Xue-Wen Tang Yang Zhou Jun-Hang Pan Hai-Yan Mao Yan-Jun Zhang Hua-Kun Lv 2020-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008574 https://doaj.org/article/a52c0dd8f58e402da2e8439c08b6fbfb EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008574 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008574 https://doaj.org/article/a52c0dd8f58e402da2e8439c08b6fbfb PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008574 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008574 2022-12-31T07:36:44Z Background Shifts have occurred in the epidemiological characteristics of Japanese encephalitis (JE), extending from the molecular level to the population level. The aim of this study was to investigate the seroprevalence of JE neutralizing antibodies in healthy populations from different age groups in Zhejiang Province, and to conduct mosquito monitoring to evaluate the infection rate of Japanese encephalitis virus (JEV) among vectors, as well as the molecular characteristics of the E gene of isolated JEV strains. Methodology/principal findings A total of 1190 sera samples were screened by a microseroneutralization test, including 429 infants (28d-11m) and 761 participants (2y-82y). For those under 1 year old, the geometric mean titers (GMTs) of the JE neutralizing antibody was 9.49 at birth and significantly declined as the age of month increased (r = -0.225, P<0.001). For those above 1-year old, seropositive proportions were higher in subjects aged 1-3 years old as well as ≥25 years old (65%-75%), and relatively lower in subjects aged between 4-25 years old (22%-55%). Four or more years after the 2nd dose of JEV-L (first dose administered at 8 months and the second at 2 years of age), the seropositive proportion decreased to 32.5%, and GMTs decreased to 8.08. A total of 87,201 mosquitoes were collected from livestock sheds in 6 surveillance sites during 2015-2018, from which 139 E gene sequences were successfully amplified. The annual infection rate according to bias-corrected maximum likelihood estimation of JEV in Culex tritaeniorhynchus was 1.56, 2.36, 5.65 and 1.77 per 1000, respectively. JEV strains isolated during 2015-2018 all belonged to Genotype I. The E gene of amplified 139 samples differed from the JEV-L vaccine strain at fourteen amino acid residues, including the eight key residues related to virulence and virus attenuation. No divergence was observed at the sites related to antigenicity. Conclusions/significance Zhejiang Province was at a high risk of JE exposure due to relatively lower ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 8 e0008574 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Xuan Deng Ju-Ying Yan Han-Qing He Rui Yan Yi Sun Xue-Wen Tang Yang Zhou Jun-Hang Pan Hai-Yan Mao Yan-Jun Zhang Hua-Kun Lv Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Shifts have occurred in the epidemiological characteristics of Japanese encephalitis (JE), extending from the molecular level to the population level. The aim of this study was to investigate the seroprevalence of JE neutralizing antibodies in healthy populations from different age groups in Zhejiang Province, and to conduct mosquito monitoring to evaluate the infection rate of Japanese encephalitis virus (JEV) among vectors, as well as the molecular characteristics of the E gene of isolated JEV strains. Methodology/principal findings A total of 1190 sera samples were screened by a microseroneutralization test, including 429 infants (28d-11m) and 761 participants (2y-82y). For those under 1 year old, the geometric mean titers (GMTs) of the JE neutralizing antibody was 9.49 at birth and significantly declined as the age of month increased (r = -0.225, P<0.001). For those above 1-year old, seropositive proportions were higher in subjects aged 1-3 years old as well as ≥25 years old (65%-75%), and relatively lower in subjects aged between 4-25 years old (22%-55%). Four or more years after the 2nd dose of JEV-L (first dose administered at 8 months and the second at 2 years of age), the seropositive proportion decreased to 32.5%, and GMTs decreased to 8.08. A total of 87,201 mosquitoes were collected from livestock sheds in 6 surveillance sites during 2015-2018, from which 139 E gene sequences were successfully amplified. The annual infection rate according to bias-corrected maximum likelihood estimation of JEV in Culex tritaeniorhynchus was 1.56, 2.36, 5.65 and 1.77 per 1000, respectively. JEV strains isolated during 2015-2018 all belonged to Genotype I. The E gene of amplified 139 samples differed from the JEV-L vaccine strain at fourteen amino acid residues, including the eight key residues related to virulence and virus attenuation. No divergence was observed at the sites related to antigenicity. Conclusions/significance Zhejiang Province was at a high risk of JE exposure due to relatively lower ... |
format |
Article in Journal/Newspaper |
author |
Xuan Deng Ju-Ying Yan Han-Qing He Rui Yan Yi Sun Xue-Wen Tang Yang Zhou Jun-Hang Pan Hai-Yan Mao Yan-Jun Zhang Hua-Kun Lv |
author_facet |
Xuan Deng Ju-Ying Yan Han-Qing He Rui Yan Yi Sun Xue-Wen Tang Yang Zhou Jun-Hang Pan Hai-Yan Mao Yan-Jun Zhang Hua-Kun Lv |
author_sort |
Xuan Deng |
title |
Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
title_short |
Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
title_full |
Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
title_fullStr |
Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
title_full_unstemmed |
Serological and molecular epidemiology of Japanese Encephalitis in Zhejiang, China, 2015-2018. |
title_sort |
serological and molecular epidemiology of japanese encephalitis in zhejiang, china, 2015-2018. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008574 https://doaj.org/article/a52c0dd8f58e402da2e8439c08b6fbfb |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008574 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008574 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008574 https://doaj.org/article/a52c0dd8f58e402da2e8439c08b6fbfb |
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https://doi.org/10.1371/journal.pntd.0008574 |
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PLOS Neglected Tropical Diseases |
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