Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats
Objective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis. Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:a4fc03399d264de993ae1f29eb72c9b1 2023-05-15T15:12:54+02:00 Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.271722 https://doaj.org/article/a4fc03399d264de993ae1f29eb72c9b1 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=12;spage=493;epage=500;aulast=Kamaruzzaman https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.271722 https://doaj.org/article/a4fc03399d264de993ae1f29eb72c9b1 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 12, Pp 493-500 (2019) kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.271722 2022-12-31T13:09:08Z Objective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis. Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative control), calcium water (positive control), kelulut honey (200 mg/kg/day and 400 mg/kg/day, respectively) treatment, respectively. All of them were administered with intramuscular injection of dexamethasone (120 μg/kg/day) to induce osteoporosis. Seven sham operated rats were given vehicle palm olein 0.05 mL/100 g/day by intramuscular injection and 0.1 mL/kg/day orally. All the treatments were given daily for 2 month. Lipid peroxidation and oxidative stress enzymes were measured. In addition, bone structural and histomorphometry analyses were also conducted. Results: Two-month glucocorticoid treatment increased the level of malondialdehyde and decreased superoxide dismutase significantly. No significant changes were found in the activities of catalase and glutathion peroxidase. Bone volume/tissue volume and trabecular number were significantly reduced while trabecular separation of the femoral bones was increased which corresponded to the decreased number of osteoblast surface after two months of receiving glucocorticoid treatment. Kelulut honey treatment restored the level of superoxide dismutase and reduced malondialdehyde significantly (P<0.05). Moreover, kelulut honey increased bone volume/tissue volume, trabecular number and decreased trabecular separation significantly (P<0.05), which were further confirmed by increased osteoblast surface and decreased osteoclast surface number (P<0.05). Conclusions: Kelulut honey may have potential bone protective effect, and may be a prophylaxis against glucocorticoid-induced osteoporosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 12 493 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
topic_facet |
kelulut honey glucocorticoid-induced osteoporosis oxidative stress antioxidant bone structure histomorphometry male rats Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To explore the effects of kelulut honey on bone structure and histomorphometry against glucocorticoid-induced osteoporosis. Methods: Thirty-five male rats were used (n = 7). Twenty-eight adrenalectomized rats were divided into four groups; each group was given normal saline 0.9% (negative control), calcium water (positive control), kelulut honey (200 mg/kg/day and 400 mg/kg/day, respectively) treatment, respectively. All of them were administered with intramuscular injection of dexamethasone (120 μg/kg/day) to induce osteoporosis. Seven sham operated rats were given vehicle palm olein 0.05 mL/100 g/day by intramuscular injection and 0.1 mL/kg/day orally. All the treatments were given daily for 2 month. Lipid peroxidation and oxidative stress enzymes were measured. In addition, bone structural and histomorphometry analyses were also conducted. Results: Two-month glucocorticoid treatment increased the level of malondialdehyde and decreased superoxide dismutase significantly. No significant changes were found in the activities of catalase and glutathion peroxidase. Bone volume/tissue volume and trabecular number were significantly reduced while trabecular separation of the femoral bones was increased which corresponded to the decreased number of osteoblast surface after two months of receiving glucocorticoid treatment. Kelulut honey treatment restored the level of superoxide dismutase and reduced malondialdehyde significantly (P<0.05). Moreover, kelulut honey increased bone volume/tissue volume, trabecular number and decreased trabecular separation significantly (P<0.05), which were further confirmed by increased osteoblast surface and decreased osteoclast surface number (P<0.05). Conclusions: Kelulut honey may have potential bone protective effect, and may be a prophylaxis against glucocorticoid-induced osteoporosis. |
format |
Article in Journal/Newspaper |
author |
Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli |
author_facet |
Mohd Amir Kamaruzzaman Amardev Thanu Mohd Rafizul Yusof Ima Nirwana Soelaiman Elvy Suhana Ramli |
author_sort |
Mohd Amir Kamaruzzaman |
title |
Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
title_short |
Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
title_full |
Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
title_fullStr |
Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
title_full_unstemmed |
Kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
title_sort |
kelulut honey ameliorates glucocorticoid induced osteoporosis via its antioxidant activity in rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.271722 https://doaj.org/article/a4fc03399d264de993ae1f29eb72c9b1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 12, Pp 493-500 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=12;spage=493;epage=500;aulast=Kamaruzzaman https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.271722 https://doaj.org/article/a4fc03399d264de993ae1f29eb72c9b1 |
op_doi |
https://doi.org/10.4103/2221-1691.271722 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
12 |
container_start_page |
493 |
_version_ |
1766343528763359232 |