Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART)
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with o...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:a4ea505474d042ddac11bc78494442ed 2023-05-15T15:19:02+02:00 Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi 2008-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992008000400011 https://doaj.org/article/a4ea505474d042ddac11bc78494442ed EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000400011 1678-9199 https://doaj.org/article/a4ea505474d042ddac11bc78494442ed Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 4, Pp 685-702 (2008) RT-PCR ELISA cytokines HIV AIDS HAART apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2008 ftdoajarticles https://doi.org/10.1590/S1678-91992008000400011 2022-12-31T09:08:52Z A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-α, IL-2, INF-γ, IL-4 and IL-10. All patients were submitted to VL determination and CD4+ and CD8+T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (> 80% r² > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-γ required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-γ in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4+T cells, mainly during primary infection, persisting only in those with INF-γ phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 14 4 685 702 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
RT-PCR ELISA cytokines HIV AIDS HAART apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
RT-PCR ELISA cytokines HIV AIDS HAART apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
topic_facet |
RT-PCR ELISA cytokines HIV AIDS HAART apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
A cross-sectional study was performed on HIV-1 infected individuals with or without antiretroviral treatment (ARV) in the AIDS Day Hospital, Botucatu Medical School, UNESP. Between August 2004 and October 2005, 73 HIV-1 infected individuals were divided into three groups: infected individuals with or without AIDS who had never received ARV (G1 = 15); patients on HAART that had had plasma HIV-1 RNA viral load (VL) equal to or greater than 50 copies/mL (G2 = 27); and patients on HAART with undetectable VL for at least the past six months (G3 = 31). There was also an additional group that comprised blood donors without any sign of the disease and with negative HIV serum tests (G4 = 20), which was the control group. Serum cytokine levels (values in pg/mL) were measured by enzyme-linked immunosorbent assay (ELISA) and specific mRNA expression by reverse transcription polymerase chain reaction (RT-PCR). Both techniques were performed on the four groups for TNF-α, IL-2, INF-γ, IL-4 and IL-10. All patients were submitted to VL determination and CD4+ and CD8+T lymphocyte counts. The analysis of the results revealed a significant comparison among groups for both methods and an association between the latter (> 80% r² > 0.80). There was only one exception, in control individuals for IL-2 by ELISA. The cytokine profiles, in both methods, for the three patient groups, were mature Th-0. The behaviors of IL-2 and INF-γ required emphasis due to consequent expression of dominant Th profile. Both methods showed low IL-2 and high mean values of INF-γ in the three groups. Several authors have recently drawn attention to the substantial apoptosis of infected and non-infected CD4+T cells, mainly during primary infection, persisting only in those with INF-γ phenotype producer and not IL-2. HIV infected individuals submitted to HAART are expected to produce IL-2 in an attempt to present Th-1 profile, but in most cases this did not occur. |
format |
Article in Journal/Newspaper |
author |
DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi |
author_facet |
DA Meira RAMB Almeida AN Barbosa LR de Souza TET Olivo RMS Henriques MA Golim JP Araújo Jr LR Nagoshi CM Orikaza SA Calvi |
author_sort |
DA Meira |
title |
Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
title_short |
Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
title_full |
Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
title_fullStr |
Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
title_full_unstemmed |
Assessment of cytokine values in serum by RT-PCR in HIV-1 infected individuals with and without highly active anti-retroviral therapy (HAART) |
title_sort |
assessment of cytokine values in serum by rt-pcr in hiv-1 infected individuals with and without highly active anti-retroviral therapy (haart) |
publisher |
SciELO |
publishDate |
2008 |
url |
https://doi.org/10.1590/S1678-91992008000400011 https://doaj.org/article/a4ea505474d042ddac11bc78494442ed |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 14, Iss 4, Pp 685-702 (2008) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000400011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992008000400011 1678-9199 https://doaj.org/article/a4ea505474d042ddac11bc78494442ed |
op_doi |
https://doi.org/10.1590/S1678-91992008000400011 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
14 |
container_issue |
4 |
container_start_page |
685 |
op_container_end_page |
702 |
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1766349218678571008 |