Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges
A total of 297 bacterial strains were isolated from five Antarctic sponge species and tested by agarose gel electrophoresis for the presence of plasmid molecules. At least one kind of plasmid was carried by 69 isolates (about 23%). The disc diffusion susceptibility test was used to assay the resista...
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2011
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ftdoajarticles:oai:doaj.org/article:a4daa29f916348549db996c2adc10549 2023-05-15T13:33:51+02:00 Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges Vivia Bruni Angelina Lo Giudice Luigi Michaud Consolazione Caruso Santina Mangano 2011-04-01T00:00:00Z https://doi.org/10.1478/C1A8901003 https://doaj.org/article/a4daa29f916348549db996c2adc10549 EN IT eng ita Accademia Peloritana dei Pericolanti http://dx.doi.org/10.1478/C1A8901003 https://doaj.org/toc/0365-0359 https://doaj.org/toc/1825-1242 doi:10.1478/C1A8901003 0365-0359 1825-1242 https://doaj.org/article/a4daa29f916348549db996c2adc10549 Atti della Accademia Peloritana dei Pericolanti : Classe di Scienze Fisiche, Matematiche e Naturali, Vol 89, Iss 1, Pp C1A8901003-1 (2011) Plasmid Antibiotic resistance Antarctica Marine Sponges Science (General) Q1-390 article 2011 ftdoajarticles https://doi.org/10.1478/C1A8901003 2022-12-31T08:13:29Z A total of 297 bacterial strains were isolated from five Antarctic sponge species and tested by agarose gel electrophoresis for the presence of plasmid molecules. At least one kind of plasmid was carried by 69 isolates (about 23%). The disc diffusion susceptibility test was used to assay the resistance of plasmid-harbouring bacteria towards 11 antibiotics. A multiple resistance was observed for the 72% of strains, among which the 33% were resistant to only two antibiotics. Bacteria showed a high degree of resistance towards O/129 (71%), tetracycline (42%) and nalidixic acid (25%), whereas any isolate showed resistance to gentamicin. The 16S rDNA sequencing revealed that plasmid-harbouring strains were mainly affiliated to the Gammaproteobacteria (81%), whereas the other detected phylogenetic groups (i.e. Firmicutes , Alphaproteobacteria , Actinobacteria and CFB group of Bacteroidetes ) were less abundant, each representing between 1% and 6% of the total isolates. The present study will contribute to the poor and fragmentary knowledge on plasmid incidence in natural microbial populations. In addition, monitoring antibiotic resistance in bacteria from remote areas, such as Antarctica, could also be a useful tool to evaluate the impact of anthropic pressure. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Italian |
topic |
Plasmid Antibiotic resistance Antarctica Marine Sponges Science (General) Q1-390 |
spellingShingle |
Plasmid Antibiotic resistance Antarctica Marine Sponges Science (General) Q1-390 Vivia Bruni Angelina Lo Giudice Luigi Michaud Consolazione Caruso Santina Mangano Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
topic_facet |
Plasmid Antibiotic resistance Antarctica Marine Sponges Science (General) Q1-390 |
description |
A total of 297 bacterial strains were isolated from five Antarctic sponge species and tested by agarose gel electrophoresis for the presence of plasmid molecules. At least one kind of plasmid was carried by 69 isolates (about 23%). The disc diffusion susceptibility test was used to assay the resistance of plasmid-harbouring bacteria towards 11 antibiotics. A multiple resistance was observed for the 72% of strains, among which the 33% were resistant to only two antibiotics. Bacteria showed a high degree of resistance towards O/129 (71%), tetracycline (42%) and nalidixic acid (25%), whereas any isolate showed resistance to gentamicin. The 16S rDNA sequencing revealed that plasmid-harbouring strains were mainly affiliated to the Gammaproteobacteria (81%), whereas the other detected phylogenetic groups (i.e. Firmicutes , Alphaproteobacteria , Actinobacteria and CFB group of Bacteroidetes ) were less abundant, each representing between 1% and 6% of the total isolates. The present study will contribute to the poor and fragmentary knowledge on plasmid incidence in natural microbial populations. In addition, monitoring antibiotic resistance in bacteria from remote areas, such as Antarctica, could also be a useful tool to evaluate the impact of anthropic pressure. |
format |
Article in Journal/Newspaper |
author |
Vivia Bruni Angelina Lo Giudice Luigi Michaud Consolazione Caruso Santina Mangano |
author_facet |
Vivia Bruni Angelina Lo Giudice Luigi Michaud Consolazione Caruso Santina Mangano |
author_sort |
Vivia Bruni |
title |
Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
title_short |
Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
title_full |
Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
title_fullStr |
Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
title_full_unstemmed |
Incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from Antarctic sponges |
title_sort |
incidence of plasmid and antibiotic resistance in psychrotrophic bacteria isolated from antarctic sponges |
publisher |
Accademia Peloritana dei Pericolanti |
publishDate |
2011 |
url |
https://doi.org/10.1478/C1A8901003 https://doaj.org/article/a4daa29f916348549db996c2adc10549 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Atti della Accademia Peloritana dei Pericolanti : Classe di Scienze Fisiche, Matematiche e Naturali, Vol 89, Iss 1, Pp C1A8901003-1 (2011) |
op_relation |
http://dx.doi.org/10.1478/C1A8901003 https://doaj.org/toc/0365-0359 https://doaj.org/toc/1825-1242 doi:10.1478/C1A8901003 0365-0359 1825-1242 https://doaj.org/article/a4daa29f916348549db996c2adc10549 |
op_doi |
https://doi.org/10.1478/C1A8901003 |
_version_ |
1766046480166027264 |