Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria
Abstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falci...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2020
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-020-03246-0 https://doaj.org/article/a4ac3c2a27fb40daa4766a8f8ab04d66 |
id |
ftdoajarticles:oai:doaj.org/article:a4ac3c2a27fb40daa4766a8f8ab04d66 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:a4ac3c2a27fb40daa4766a8f8ab04d66 2023-05-15T15:08:52+02:00 Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga 2020-05-01T00:00:00Z https://doi.org/10.1186/s12936-020-03246-0 https://doaj.org/article/a4ac3c2a27fb40daa4766a8f8ab04d66 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03246-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03246-0 1475-2875 https://doaj.org/article/a4ac3c2a27fb40daa4766a8f8ab04d66 Malaria Journal, Vol 19, Iss 1, Pp 1-6 (2020) Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03246-0 2022-12-31T11:11:22Z Abstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. Methods A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. Results The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. Conclusion This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
topic_facet |
Plasmodium falciparum Pfmdr1 Antimalarial drug resistance Lumefantrine Brazzaville Republic of Congo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria in pregnancy is associated with considerable morbidity and mortality. Regular surveillance of artemisinin-based combination therapy tolerance, or molecular makers of resistance, is vital for effective malaria treatment, control and eradication programmes. Plasmodium falciparum multiple drug resistance-1 gene (Pfmdr1) N86Y mutation is associated with reduced susceptibility to lumefantrine. This study assessed the prevalence of Pfmdr1 N86Y in Brazzaville, Republic of Congo. Methods A total 1001 of P. falciparum-infected blood samples obtained from asymptomatic malaria pregnant women having a normal child delivery at the Madibou Integrated Health Centre were analysed. Pfmdr1 N86Y genotyping was conducted using PCR-restriction fragment length polymorphism. Results The wild type Pfmdr1 N86 allele was predominant (> 68%) in this study, whereas a few isolates carrying the either the mutant allele (Pfmdr1 86Y) alone or both alleles (mixed genotype). The dominance of the wildtype allele (pfmdr1 N86) indicates the plausible decline P. falciparum susceptibility to lumefantrine. Conclusion This study gives an update on the prevalence of Pfmdr1 N86Y alleles in Brazzaville, Republic of Congo. It also raises concern on the imminent emergence of resistance against artemether–lumefantrine in this setting. This study underscores the importance to regular artemether–lumefantrine efficacy monitoring to inform the malaria control programme of the Republic of Congo. |
format |
Article in Journal/Newspaper |
author |
Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga |
author_facet |
Louis Regis Dossou-Yovo Francine Ntoumi Felix Koukouikila-Koussounda Jeannhey Christevy Vouvoungui Ayodele Adedoja David Nderu Thirumalaisamy P. Velavan Arsène Lenga |
author_sort |
Louis Regis Dossou-Yovo |
title |
Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_short |
Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_full |
Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_fullStr |
Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_full_unstemmed |
Molecular surveillance of the Pfmdr1 N86Y allele among Congolese pregnant women with asymptomatic malaria |
title_sort |
molecular surveillance of the pfmdr1 n86y allele among congolese pregnant women with asymptomatic malaria |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03246-0 https://doaj.org/article/a4ac3c2a27fb40daa4766a8f8ab04d66 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-6 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03246-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03246-0 1475-2875 https://doaj.org/article/a4ac3c2a27fb40daa4766a8f8ab04d66 |
op_doi |
https://doi.org/10.1186/s12936-020-03246-0 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
_version_ |
1766340148344127488 |