Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition
Abstract Background The salmon louse ( Lepeophtheirus salmonis Krøyer), an ectoparasitic copepod with a complex life cycle causes significant losses in salmon aquaculture. Pesticide treatments against the parasite raise environmental concerns and their efficacy is gradually decreasing. Improvement o...
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ftdoajarticles:oai:doaj.org/article:a4923cafe6be421c9fba0994e9486213 2023-05-15T15:32:04+02:00 Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition Afanasyev Sergey Skugor Stanko Krasnov Aleksei Tadiso Tariku Hordvik Ivar Nilsen Frank 2011-03-01T00:00:00Z https://doi.org/10.1186/1471-2164-12-141 https://doaj.org/article/a4923cafe6be421c9fba0994e9486213 EN eng BMC http://www.biomedcentral.com/1471-2164/12/141 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-12-141 1471-2164 https://doaj.org/article/a4923cafe6be421c9fba0994e9486213 BMC Genomics, Vol 12, Iss 1, p 141 (2011) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2011 ftdoajarticles https://doi.org/10.1186/1471-2164-12-141 2022-12-31T08:35:36Z Abstract Background The salmon louse ( Lepeophtheirus salmonis Krøyer), an ectoparasitic copepod with a complex life cycle causes significant losses in salmon aquaculture. Pesticide treatments against the parasite raise environmental concerns and their efficacy is gradually decreasing. Improvement of fish resistance to lice, through biological control methods, needs better understanding of the protective mechanisms. We used a 21 k oligonucleotide microarray and RT-qPCR to examine the time-course of immune gene expression changes in salmon skin, spleen, and head kidney during the first 15 days after challenge, which encompassed the copepod and chalimus stages of lice development. Results Large scale and highly complex transcriptome responses were found already one day after infection (dpi). Many genes showed bi-phasic expression profiles with abrupt changes between 5 and 10 dpi (the copepod-chalimus transitions); the greatest fluctuations (up- and down-regulation) were seen in a large group of secretory splenic proteases with unknown roles. Rapid sensing was witnessed with induction of genes involved in innate immunity including lectins and enzymes of eicosanoid metabolism in skin and acute phase proteins in spleen. Transient (1-5 dpi) increase of T-cell receptor alpha, CD4-1, and possible regulators of lymphocyte differentiation suggested recruitment of T-cells of unidentified lineage to the skin. After 5 dpi the magnitude of transcriptomic responses decreased markedly in skin. Up-regulation of matrix metalloproteinases in all studied organs suggested establishment of a chronic inflammatory status. Up-regulation of putative lymphocyte G0/G1 switch proteins in spleen at 5 dpi, immunoglobulins at 15 dpi; and increase of IgM and IgT transcripts in skin indicated an onset of adaptive humoral immune responses, whereas MHCI appeared to be down-regulated. Conclusions Atlantic salmon develops rapid local and systemic reactions to L. salmonis , which, however, do not result in substantial level of protection. The ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles BMC Genomics 12 1 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Afanasyev Sergey Skugor Stanko Krasnov Aleksei Tadiso Tariku Hordvik Ivar Nilsen Frank Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background The salmon louse ( Lepeophtheirus salmonis Krøyer), an ectoparasitic copepod with a complex life cycle causes significant losses in salmon aquaculture. Pesticide treatments against the parasite raise environmental concerns and their efficacy is gradually decreasing. Improvement of fish resistance to lice, through biological control methods, needs better understanding of the protective mechanisms. We used a 21 k oligonucleotide microarray and RT-qPCR to examine the time-course of immune gene expression changes in salmon skin, spleen, and head kidney during the first 15 days after challenge, which encompassed the copepod and chalimus stages of lice development. Results Large scale and highly complex transcriptome responses were found already one day after infection (dpi). Many genes showed bi-phasic expression profiles with abrupt changes between 5 and 10 dpi (the copepod-chalimus transitions); the greatest fluctuations (up- and down-regulation) were seen in a large group of secretory splenic proteases with unknown roles. Rapid sensing was witnessed with induction of genes involved in innate immunity including lectins and enzymes of eicosanoid metabolism in skin and acute phase proteins in spleen. Transient (1-5 dpi) increase of T-cell receptor alpha, CD4-1, and possible regulators of lymphocyte differentiation suggested recruitment of T-cells of unidentified lineage to the skin. After 5 dpi the magnitude of transcriptomic responses decreased markedly in skin. Up-regulation of matrix metalloproteinases in all studied organs suggested establishment of a chronic inflammatory status. Up-regulation of putative lymphocyte G0/G1 switch proteins in spleen at 5 dpi, immunoglobulins at 15 dpi; and increase of IgM and IgT transcripts in skin indicated an onset of adaptive humoral immune responses, whereas MHCI appeared to be down-regulated. Conclusions Atlantic salmon develops rapid local and systemic reactions to L. salmonis , which, however, do not result in substantial level of protection. The ... |
format |
Article in Journal/Newspaper |
author |
Afanasyev Sergey Skugor Stanko Krasnov Aleksei Tadiso Tariku Hordvik Ivar Nilsen Frank |
author_facet |
Afanasyev Sergey Skugor Stanko Krasnov Aleksei Tadiso Tariku Hordvik Ivar Nilsen Frank |
author_sort |
Afanasyev Sergey |
title |
Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
title_short |
Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
title_full |
Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
title_fullStr |
Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
title_full_unstemmed |
Gene expression analyses of immune responses in Atlantic salmon during early stages of infection by salmon louse ( Lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
title_sort |
gene expression analyses of immune responses in atlantic salmon during early stages of infection by salmon louse ( lepeophtheirus salmonis ) revealed bi-phasic responses coinciding with the copepod-chalimus transition |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1471-2164-12-141 https://doaj.org/article/a4923cafe6be421c9fba0994e9486213 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
BMC Genomics, Vol 12, Iss 1, p 141 (2011) |
op_relation |
http://www.biomedcentral.com/1471-2164/12/141 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-12-141 1471-2164 https://doaj.org/article/a4923cafe6be421c9fba0994e9486213 |
op_doi |
https://doi.org/10.1186/1471-2164-12-141 |
container_title |
BMC Genomics |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766362574600798208 |