Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection
Abstract Background Laboratory studies have demonstrated that a variety of immune signaling pathways regulate malaria parasite infection in Anopheles gambiae , the primary vector species in Africa. Methods To begin to understand the importance of these associations under natural conditions, an assoc...
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ftdoajarticles:oai:doaj.org/article:a4795a75b53a4fbe906d67e305c41ca0 2023-05-15T15:08:21+02:00 Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection Lanzaro Gregory C Cornel Anthony J Rashbrook Vanessa K Coulibaly Cheick A Lee Yoosook Horton Ashley A Luckhart Shirley 2010-06-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-160 https://doaj.org/article/a4795a75b53a4fbe906d67e305c41ca0 EN eng BMC http://www.malariajournal.com/content/9/1/160 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-160 1475-2875 https://doaj.org/article/a4795a75b53a4fbe906d67e305c41ca0 Malaria Journal, Vol 9, Iss 1, p 160 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-160 2022-12-31T08:44:14Z Abstract Background Laboratory studies have demonstrated that a variety of immune signaling pathways regulate malaria parasite infection in Anopheles gambiae , the primary vector species in Africa. Methods To begin to understand the importance of these associations under natural conditions, an association mapping approach was adopted to determine whether single nucleotide polymorphisms (SNPs) in selected immune signaling genes in A. gambiae collected in Mali were associated with the phenotype of Plasmodium falciparum infection. Results Three SNPs were identified in field-collected mosquitoes that were associated with parasite infection in molecular form-dependent patterns: two were detected in the Toll5B gene and one was detected in the gene encoding insulin-like peptide 3 precursor. In addition, one infection-associated Toll5B SNP was in linkage disequilibrium with a SNP in sequence encoding a mitogen-activated protein kinase that has been associated with Toll signaling in mammalian cells. Both Toll5B SNPs showed divergence from Hardy-Weinberg equilibrium, suggesting that selection pressure(s) are acting on these loci. Conclusions Seven of these eight infection-associated and linked SNPs alter codon frequency or introduce non-synonymous changes that would be predicted to alter protein structure and, hence, function, suggesting that these SNPs could alter immune signaling and responsiveness to parasite infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 24 4 209 216 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Lanzaro Gregory C Cornel Anthony J Rashbrook Vanessa K Coulibaly Cheick A Lee Yoosook Horton Ashley A Luckhart Shirley Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Laboratory studies have demonstrated that a variety of immune signaling pathways regulate malaria parasite infection in Anopheles gambiae , the primary vector species in Africa. Methods To begin to understand the importance of these associations under natural conditions, an association mapping approach was adopted to determine whether single nucleotide polymorphisms (SNPs) in selected immune signaling genes in A. gambiae collected in Mali were associated with the phenotype of Plasmodium falciparum infection. Results Three SNPs were identified in field-collected mosquitoes that were associated with parasite infection in molecular form-dependent patterns: two were detected in the Toll5B gene and one was detected in the gene encoding insulin-like peptide 3 precursor. In addition, one infection-associated Toll5B SNP was in linkage disequilibrium with a SNP in sequence encoding a mitogen-activated protein kinase that has been associated with Toll signaling in mammalian cells. Both Toll5B SNPs showed divergence from Hardy-Weinberg equilibrium, suggesting that selection pressure(s) are acting on these loci. Conclusions Seven of these eight infection-associated and linked SNPs alter codon frequency or introduce non-synonymous changes that would be predicted to alter protein structure and, hence, function, suggesting that these SNPs could alter immune signaling and responsiveness to parasite infection. |
format |
Article in Journal/Newspaper |
author |
Lanzaro Gregory C Cornel Anthony J Rashbrook Vanessa K Coulibaly Cheick A Lee Yoosook Horton Ashley A Luckhart Shirley |
author_facet |
Lanzaro Gregory C Cornel Anthony J Rashbrook Vanessa K Coulibaly Cheick A Lee Yoosook Horton Ashley A Luckhart Shirley |
author_sort |
Lanzaro Gregory C |
title |
Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
title_short |
Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
title_full |
Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
title_fullStr |
Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
title_full_unstemmed |
Identification of three single nucleotide polymorphisms in Anopheles gambiae immune signaling genes that are associated with natural Plasmodium falciparum infection |
title_sort |
identification of three single nucleotide polymorphisms in anopheles gambiae immune signaling genes that are associated with natural plasmodium falciparum infection |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-160 https://doaj.org/article/a4795a75b53a4fbe906d67e305c41ca0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 160 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/160 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-160 1475-2875 https://doaj.org/article/a4795a75b53a4fbe906d67e305c41ca0 |
op_doi |
https://doi.org/10.1186/1475-2875-9-160 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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24 |
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4 |
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209 |
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216 |
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