Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi
Abstract Background In areas co-endemic for multiple Plasmodium species, correct diagnosis is crucial for appropriate treatment and surveillance. Species misidentification by microscopy has been reported in areas co-endemic for vivax and falciparum malaria, and may be more frequent in regions where...
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ftdoajarticles:oai:doaj.org/article:a471c105e212409abb9c08ae09781e60 2023-05-15T15:17:58+02:00 Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi Barber Bridget E William Timothy Grigg Matthew J Yeo Tsin W Anstey Nicholas M 2013-01-01T00:00:00Z https://doi.org/10.1186/1475-2875-12-8 https://doaj.org/article/a471c105e212409abb9c08ae09781e60 EN eng BMC http://www.malariajournal.com/content/12/1/8 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-12-8 1475-2875 https://doaj.org/article/a471c105e212409abb9c08ae09781e60 Malaria Journal, Vol 12, Iss 1, p 8 (2013) Plasmodium knowlesi Malaria Microscopy Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles https://doi.org/10.1186/1475-2875-12-8 2022-12-30T22:59:39Z Abstract Background In areas co-endemic for multiple Plasmodium species, correct diagnosis is crucial for appropriate treatment and surveillance. Species misidentification by microscopy has been reported in areas co-endemic for vivax and falciparum malaria, and may be more frequent in regions where Plasmodium knowlesi also commonly occurs. Methods This prospective study in Sabah, Malaysia, evaluated the accuracy of routine district and referral hospital-based microscopy, and microscopy performed by an experienced research microscopist, for the diagnosis of PCR-confirmed Plasmodium falciparum , P . knowlesi , and Plasmodium vivax malaria. Results A total of 304 patients with PCR-confirmed Plasmodium infection were enrolled, including 130 with P . knowlesi , 122 with P . falciparum , 43 with P . vivax , one with Plasmodium malariae and eight with mixed species infections. Among patients with P . knowlesi mono-infection, routine and cross-check microscopy both identified 94 (72%) patients as “ P . malariae / P . knowlesi ”; 17 (13%) and 28 (22%) respectively were identified as P . falciparum , and 13 (10%) and two (1.5%) as P . vivax . Among patients with PCR-confirmed P . falciparum , routine and cross-check microscopy identified 110/122 (90%) and 112/118 (95%) patients respectively as P . falciparum , and 8/122 (6.6%) and 5/118 (4.2%) as “ P . malariae / P . knowlesi ”. Among those with P . vivax , 23/43 (53%) and 34/40 (85%) were correctly diagnosed by routine and cross-check microscopy respectively, while 13/43 (30%) and 3/40 (7.5%) patients were diagnosed as “ P . malariae / P . knowlesi ”. Four of 13 patients with PCR-confirmed P . vivax and misdiagnosed by routine microscopy as “ P . malariae / P . knowlesi ” were subsequently re-admitted with P . vivax malaria. Conclusions Microscopy does not reliably distinguish between P . falciparum , P . vivax and P . knowlesi in a region where all three species frequently occur. Misdiagnosis of P . knowlesi as both P . vivax and P . falciparum , and vice versa , is ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 12 1 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
Plasmodium knowlesi Malaria Microscopy Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium knowlesi Malaria Microscopy Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Barber Bridget E William Timothy Grigg Matthew J Yeo Tsin W Anstey Nicholas M Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
topic_facet |
Plasmodium knowlesi Malaria Microscopy Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In areas co-endemic for multiple Plasmodium species, correct diagnosis is crucial for appropriate treatment and surveillance. Species misidentification by microscopy has been reported in areas co-endemic for vivax and falciparum malaria, and may be more frequent in regions where Plasmodium knowlesi also commonly occurs. Methods This prospective study in Sabah, Malaysia, evaluated the accuracy of routine district and referral hospital-based microscopy, and microscopy performed by an experienced research microscopist, for the diagnosis of PCR-confirmed Plasmodium falciparum , P . knowlesi , and Plasmodium vivax malaria. Results A total of 304 patients with PCR-confirmed Plasmodium infection were enrolled, including 130 with P . knowlesi , 122 with P . falciparum , 43 with P . vivax , one with Plasmodium malariae and eight with mixed species infections. Among patients with P . knowlesi mono-infection, routine and cross-check microscopy both identified 94 (72%) patients as “ P . malariae / P . knowlesi ”; 17 (13%) and 28 (22%) respectively were identified as P . falciparum , and 13 (10%) and two (1.5%) as P . vivax . Among patients with PCR-confirmed P . falciparum , routine and cross-check microscopy identified 110/122 (90%) and 112/118 (95%) patients respectively as P . falciparum , and 8/122 (6.6%) and 5/118 (4.2%) as “ P . malariae / P . knowlesi ”. Among those with P . vivax , 23/43 (53%) and 34/40 (85%) were correctly diagnosed by routine and cross-check microscopy respectively, while 13/43 (30%) and 3/40 (7.5%) patients were diagnosed as “ P . malariae / P . knowlesi ”. Four of 13 patients with PCR-confirmed P . vivax and misdiagnosed by routine microscopy as “ P . malariae / P . knowlesi ” were subsequently re-admitted with P . vivax malaria. Conclusions Microscopy does not reliably distinguish between P . falciparum , P . vivax and P . knowlesi in a region where all three species frequently occur. Misdiagnosis of P . knowlesi as both P . vivax and P . falciparum , and vice versa , is ... |
format |
Article in Journal/Newspaper |
author |
Barber Bridget E William Timothy Grigg Matthew J Yeo Tsin W Anstey Nicholas M |
author_facet |
Barber Bridget E William Timothy Grigg Matthew J Yeo Tsin W Anstey Nicholas M |
author_sort |
Barber Bridget E |
title |
Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
title_short |
Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
title_full |
Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
title_fullStr |
Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
title_full_unstemmed |
Limitations of microscopy to differentiate Plasmodium species in a region co-endemic for Plasmodium falciparum , Plasmodium vivax and Plasmodium knowlesi |
title_sort |
limitations of microscopy to differentiate plasmodium species in a region co-endemic for plasmodium falciparum , plasmodium vivax and plasmodium knowlesi |
publisher |
BMC |
publishDate |
2013 |
url |
https://doi.org/10.1186/1475-2875-12-8 https://doaj.org/article/a471c105e212409abb9c08ae09781e60 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 12, Iss 1, p 8 (2013) |
op_relation |
http://www.malariajournal.com/content/12/1/8 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-12-8 1475-2875 https://doaj.org/article/a471c105e212409abb9c08ae09781e60 |
op_doi |
https://doi.org/10.1186/1475-2875-12-8 |
container_title |
Malaria Journal |
container_volume |
12 |
container_issue |
1 |
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1766348212474478592 |