Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae)
A new cell line designated LSB-AA695BB, was established from embryos of the mosquito Anopheles albimanus. The primary culture was initiated in April, 1995, and the first passage was made 48 days later. Serial subcultures of the cells have been carried through 90 passages from Abril 1995 to February...
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Instituto Oswaldo Cruz, Ministério da Saúde
1997
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ftdoajarticles:oai:doaj.org/article:a45b96dae3924702b4a47c7fd2d1dd12 2023-05-15T15:04:14+02:00 Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) Felio J Bello Helena Brochero Jorge Boshell Victor Olano Gloria Rey 1997-01-01T00:00:00Z https://doi.org/10.1590/S0074-02761997000100027 https://doaj.org/article/a45b96dae3924702b4a47c7fd2d1dd12 EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100027 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761997000100027 0074-0276 1678-8060 https://doaj.org/article/a45b96dae3924702b4a47c7fd2d1dd12 Memórias do Instituto Oswaldo Cruz., Vol 92, Iss 1, p 123 (1997) cell line karyotype isozyme patterns microsporidia Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 1997 ftdoajarticles https://doi.org/10.1590/S0074-02761997000100027 2023-01-08T01:27:44Z A new cell line designated LSB-AA695BB, was established from embryos of the mosquito Anopheles albimanus. The primary culture was initiated in April, 1995, and the first passage was made 48 days later. Serial subcultures of the cells have been carried through 90 passages from Abril 1995 to February 1996. The cells were grown at 28°C in MK/VP12 medium, supplemented with 20% fetal bovine serum; the pH tolerance ranged between 6.8 to 7.0. The cells have also been adapted to MM/VP12 medium under the same pH, temperature and serum concentration. The majority of the cells were a fibroblast-type. Isozyme characterization showed a pattern similar to that of An. albimanus pupae and adults but distinct from Ae. taeniorhynchus and Ae. albopictus (C6/36) mosquito cell lines. The culture was shown to be free of mycoplasma, bacteria and fungi. Microsporidia contamination of transovarial transmission was controlled with 6.0 mg/ml of albendazole Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 92 1 123 128 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
cell line karyotype isozyme patterns microsporidia Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
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cell line karyotype isozyme patterns microsporidia Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Felio J Bello Helena Brochero Jorge Boshell Victor Olano Gloria Rey Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
topic_facet |
cell line karyotype isozyme patterns microsporidia Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
A new cell line designated LSB-AA695BB, was established from embryos of the mosquito Anopheles albimanus. The primary culture was initiated in April, 1995, and the first passage was made 48 days later. Serial subcultures of the cells have been carried through 90 passages from Abril 1995 to February 1996. The cells were grown at 28°C in MK/VP12 medium, supplemented with 20% fetal bovine serum; the pH tolerance ranged between 6.8 to 7.0. The cells have also been adapted to MM/VP12 medium under the same pH, temperature and serum concentration. The majority of the cells were a fibroblast-type. Isozyme characterization showed a pattern similar to that of An. albimanus pupae and adults but distinct from Ae. taeniorhynchus and Ae. albopictus (C6/36) mosquito cell lines. The culture was shown to be free of mycoplasma, bacteria and fungi. Microsporidia contamination of transovarial transmission was controlled with 6.0 mg/ml of albendazole |
format |
Article in Journal/Newspaper |
author |
Felio J Bello Helena Brochero Jorge Boshell Victor Olano Gloria Rey |
author_facet |
Felio J Bello Helena Brochero Jorge Boshell Victor Olano Gloria Rey |
author_sort |
Felio J Bello |
title |
Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
title_short |
Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
title_full |
Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
title_fullStr |
Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
title_full_unstemmed |
Establishment and Characterization of a Cell Line from the Mosquito Anopheles albimanus (Diptera: Culicidae) |
title_sort |
establishment and characterization of a cell line from the mosquito anopheles albimanus (diptera: culicidae) |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
1997 |
url |
https://doi.org/10.1590/S0074-02761997000100027 https://doaj.org/article/a45b96dae3924702b4a47c7fd2d1dd12 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 92, Iss 1, p 123 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100027 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02761997000100027 0074-0276 1678-8060 https://doaj.org/article/a45b96dae3924702b4a47c7fd2d1dd12 |
op_doi |
https://doi.org/10.1590/S0074-02761997000100027 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
92 |
container_issue |
1 |
container_start_page |
123 |
op_container_end_page |
128 |
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1766336036963614720 |