Antibacterial activity of naringenin-rich fraction of pigeon pea leaves toward Salmonella thypi

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Sarifa Agus, Suminar Setiati Achmadi, Nisa Rachmania Mubarik
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Antibacterial activity; Cajanus cajan; Flavanones; Naringenin; Salmonella thypi; Typhoid; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.07.019; https://doaj.org/article/a447b9ec356b41ed93de9ef7f60c5858

Objective: To identify bioactive compound in pigeon pea leaves (Cajanus cajan) that inhibits Salmonella thypi (S. thypi). Methods: The leaf sample was powdered and macerated with methanol and fractioned by liquid–liquid extraction using ethyl acetate. The fraction was chromatographed and the isolates were identified for major component with liquid chromatography-mass spectrometry and the antibacterial activity was tested against S. thypi by Kirby–Bauer method. Results: Subfraction 1 from the ethyl acetate fraction formed a yellowish solid with m/z 272, identified as naringenin. The naringenin-rich fraction shows fairly well inhibitory toward S. thypi in comparison with chloramphenicol. Conclusions: Naringenin shows antibacterial activity and can be developed to treat typhoid.