Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo.
Borrelia persica, a bacterium transmitted by the soft tick Ornithodoros tholozani, causes tick-borne relapsing fever in humans in the Middle East, Central Asia and the Indian peninsula. Immunocompetent C3H/HeOuJ mice were infected intradermally with B. persica at varying doses: 1 x 10(6), 1 x 10(4),...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0004404 https://doaj.org/article/a44681edb51f4e6b92c4f50e52f811dc |
id |
ftdoajarticles:oai:doaj.org/article:a44681edb51f4e6b92c4f50e52f811dc |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:a44681edb51f4e6b92c4f50e52f811dc 2023-05-15T15:12:25+02:00 Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. Sandra Schwarzer Evelyn Overzier Walter Hermanns Gad Baneth Reinhard K Straubinger 2016-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004404 https://doaj.org/article/a44681edb51f4e6b92c4f50e52f811dc EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4758604?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004404 https://doaj.org/article/a44681edb51f4e6b92c4f50e52f811dc PLoS Neglected Tropical Diseases, Vol 10, Iss 2, p e0004404 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004404 2023-01-08T01:28:25Z Borrelia persica, a bacterium transmitted by the soft tick Ornithodoros tholozani, causes tick-borne relapsing fever in humans in the Middle East, Central Asia and the Indian peninsula. Immunocompetent C3H/HeOuJ mice were infected intradermally with B. persica at varying doses: 1 x 10(6), 1 x 10(4), 1 x 10(2) and 4 x 10(0) spirochetes/mouse. Subsequently, blood samples were collected and screened for the presence of B. persica DNA. Spirochetes were detected in all mice infected with 1 x 10(6), 1 x 10(4) and 1 x 10(2) borrelia by real-time PCR targeting the flaB gene of the bacterium. Spirochetemia developed with a one- to two-day delay when 1 x 10(4) and 1 x 10(2) borrelia were inoculated. Mice injected with only four organisms were negative in all tests. No clinical signs were observed when infected mice were compared to negative control animals. Organs (heart, spleen, urinary bladder, tarsal joint, skin and brain) were tested for B. persica-specific DNA and cultured for the detection of viable spirochetes. Compiled data show that the target organs of B. persica infections are the brain and the skin. A newly developed serological two-tiered test system (ELISA and western blot) for the detection of murine IgM, IgG and IgA antibody titers against B. persica showed a vigorous antibody response of the mice during infection. In conclusion, the infection model described here for B. persica is a platform for in vivo studies to decipher the so far unexplored survival strategies of this Borrelia species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian PLOS Neglected Tropical Diseases 10 2 e0004404 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sandra Schwarzer Evelyn Overzier Walter Hermanns Gad Baneth Reinhard K Straubinger Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Borrelia persica, a bacterium transmitted by the soft tick Ornithodoros tholozani, causes tick-borne relapsing fever in humans in the Middle East, Central Asia and the Indian peninsula. Immunocompetent C3H/HeOuJ mice were infected intradermally with B. persica at varying doses: 1 x 10(6), 1 x 10(4), 1 x 10(2) and 4 x 10(0) spirochetes/mouse. Subsequently, blood samples were collected and screened for the presence of B. persica DNA. Spirochetes were detected in all mice infected with 1 x 10(6), 1 x 10(4) and 1 x 10(2) borrelia by real-time PCR targeting the flaB gene of the bacterium. Spirochetemia developed with a one- to two-day delay when 1 x 10(4) and 1 x 10(2) borrelia were inoculated. Mice injected with only four organisms were negative in all tests. No clinical signs were observed when infected mice were compared to negative control animals. Organs (heart, spleen, urinary bladder, tarsal joint, skin and brain) were tested for B. persica-specific DNA and cultured for the detection of viable spirochetes. Compiled data show that the target organs of B. persica infections are the brain and the skin. A newly developed serological two-tiered test system (ELISA and western blot) for the detection of murine IgM, IgG and IgA antibody titers against B. persica showed a vigorous antibody response of the mice during infection. In conclusion, the infection model described here for B. persica is a platform for in vivo studies to decipher the so far unexplored survival strategies of this Borrelia species. |
format |
Article in Journal/Newspaper |
author |
Sandra Schwarzer Evelyn Overzier Walter Hermanns Gad Baneth Reinhard K Straubinger |
author_facet |
Sandra Schwarzer Evelyn Overzier Walter Hermanns Gad Baneth Reinhard K Straubinger |
author_sort |
Sandra Schwarzer |
title |
Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
title_short |
Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
title_full |
Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
title_fullStr |
Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
title_full_unstemmed |
Borrelia persica Infection in Immunocompetent Mice--A New Tool to Study the Infection Kinetics In Vivo. |
title_sort |
borrelia persica infection in immunocompetent mice--a new tool to study the infection kinetics in vivo. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004404 https://doaj.org/article/a44681edb51f4e6b92c4f50e52f811dc |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 2, p e0004404 (2016) |
op_relation |
http://europepmc.org/articles/PMC4758604?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004404 https://doaj.org/article/a44681edb51f4e6b92c4f50e52f811dc |
op_doi |
https://doi.org/10.1371/journal.pntd.0004404 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
2 |
container_start_page |
e0004404 |
_version_ |
1766343105119780864 |