Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype.
Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic shock syndrome. Dengue vaccine development is challenging because of the need to induce protection against four antigenically distinct DENV serotypes. Recent studies indicate that tetravalent DENV vaccines must induce...
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ftdoajarticles:oai:doaj.org/article:a436f753500142288d12edd9e04f8822 2023-05-15T15:07:12+02:00 Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. Stefan W Metz Ashlie Thomas Alex Brackbill Yi Xianwen Michele Stone Katie Horvath Michael J Miley Chris Luft Joseph M DeSimone Shaomin Tian Aravinda M de Silva 2018-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006793 https://doaj.org/article/a436f753500142288d12edd9e04f8822 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6171938?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006793 https://doaj.org/article/a436f753500142288d12edd9e04f8822 PLoS Neglected Tropical Diseases, Vol 12, Iss 9, p e0006793 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006793 2022-12-30T22:30:04Z Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic shock syndrome. Dengue vaccine development is challenging because of the need to induce protection against four antigenically distinct DENV serotypes. Recent studies indicate that tetravalent DENV vaccines must induce balanced, serotype-specific neutralizing antibodies to achieve durable protective immunity against all 4 serotypes. With the leading live attenuated tetravalent DENV vaccines, it has been difficult to achieve balanced and type-specific responses to each serotype, most likely because of unbalanced replication of vaccine viral strains. Here we evaluate a tetravalent DENV protein subunit vaccine, based on recombinant envelope protein (rE) adsorbed to the surface of poly (lactic-co-glycolic acid) (PLGA) nanoparticles for immunogenicity in mice. In monovalent and tetravalent formulations, we show that particulate rE induced higher neutralizing antibody titers compared to the soluble rE antigen alone. Importantly, we show the trend that tetravalent rE adsorbed to nanoparticles stimulated a more balanced serotype specific antibody response to each DENV serotype compared to soluble antigens. Our results demonstrate that tetravalent DENV subunit vaccines displayed on nanoparticles have the potential to overcome unbalanced immunity observed for leading live-attenuated vaccine candidates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 9 e0006793 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Stefan W Metz Ashlie Thomas Alex Brackbill Yi Xianwen Michele Stone Katie Horvath Michael J Miley Chris Luft Joseph M DeSimone Shaomin Tian Aravinda M de Silva Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Dengue virus (DENV) is the causative agent of dengue fever and dengue hemorrhagic shock syndrome. Dengue vaccine development is challenging because of the need to induce protection against four antigenically distinct DENV serotypes. Recent studies indicate that tetravalent DENV vaccines must induce balanced, serotype-specific neutralizing antibodies to achieve durable protective immunity against all 4 serotypes. With the leading live attenuated tetravalent DENV vaccines, it has been difficult to achieve balanced and type-specific responses to each serotype, most likely because of unbalanced replication of vaccine viral strains. Here we evaluate a tetravalent DENV protein subunit vaccine, based on recombinant envelope protein (rE) adsorbed to the surface of poly (lactic-co-glycolic acid) (PLGA) nanoparticles for immunogenicity in mice. In monovalent and tetravalent formulations, we show that particulate rE induced higher neutralizing antibody titers compared to the soluble rE antigen alone. Importantly, we show the trend that tetravalent rE adsorbed to nanoparticles stimulated a more balanced serotype specific antibody response to each DENV serotype compared to soluble antigens. Our results demonstrate that tetravalent DENV subunit vaccines displayed on nanoparticles have the potential to overcome unbalanced immunity observed for leading live-attenuated vaccine candidates. |
format |
Article in Journal/Newspaper |
author |
Stefan W Metz Ashlie Thomas Alex Brackbill Yi Xianwen Michele Stone Katie Horvath Michael J Miley Chris Luft Joseph M DeSimone Shaomin Tian Aravinda M de Silva |
author_facet |
Stefan W Metz Ashlie Thomas Alex Brackbill Yi Xianwen Michele Stone Katie Horvath Michael J Miley Chris Luft Joseph M DeSimone Shaomin Tian Aravinda M de Silva |
author_sort |
Stefan W Metz |
title |
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
title_short |
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
title_full |
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
title_fullStr |
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
title_full_unstemmed |
Nanoparticle delivery of a tetravalent E protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
title_sort |
nanoparticle delivery of a tetravalent e protein subunit vaccine induces balanced, type-specific neutralizing antibodies to each dengue virus serotype. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006793 https://doaj.org/article/a436f753500142288d12edd9e04f8822 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 9, p e0006793 (2018) |
op_relation |
http://europepmc.org/articles/PMC6171938?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006793 https://doaj.org/article/a436f753500142288d12edd9e04f8822 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006793 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
9 |
container_start_page |
e0006793 |
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1766338757738364928 |