Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice
Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid (4:1). Methods: The study included chromosomal aberration analysis in bone-marrow and mouse spermatocytes, inducti...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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ftdoajarticles:oai:doaj.org/article:a417a6015b574996b1786681589fa0c8 2023-05-15T15:15:22+02:00 Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice Maha A. Fahmy Ayman A. Farghaly Enayat A. Omara Zeinab M. Hassan Fawzia A.E. Aly Souria M. Donya Aziza A.E. Ibrahim Elsayed M. Bayoumy 2017-09-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.08.002 https://doaj.org/article/a417a6015b574996b1786681589fa0c8 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169117308390 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.08.002 https://doaj.org/article/a417a6015b574996b1786681589fa0c8 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 9, Pp 809-816 (2017) Amoxicillin/clavulanic acid Genotoxicity Sperm abnormalities Histopathological changes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.08.002 2022-12-31T13:32:52Z Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid (4:1). Methods: The study included chromosomal aberration analysis in bone-marrow and mouse spermatocytes, induction of sperm morphological abnormalities and histopathological changes in different body organs. The drug was administrated orally at a dose of 81 mg/kg body weight twice daily (Total = 162 mg/kg/day) for various periods of time equivalent to 625 mg/men (twice daily). Results: The results revealed non-significant chromosomal aberrations induced after treatment with amoxicillin/clavulanic acid (AC) in both bone marrow and mouse spermatocytes after 7 and 10 days treatment. On the other hand, statistically significant percentages of sperm morphological abnormalities were recorded. Such percentage reached 8.10 ± 0.55, 9.86 ± 0.63 and 12.12 ± 0.58 at the three time intervals tested (7, 14 and 35 days after the 1st treatment respectively) (treatment performed for 5 successive days) compared with 2.78 ± 0.48 for the control. The results also revealed histopathological changes in different body organs after AC treatment which increased with the prolongation of the period of therapy. Congestion of central vain, liver hemorrhage and hydropic changes in hepatocytes were noticed in the liver. Degenerative changes were found in kidney glomerulus and tubules while testis showed atrophy of seminiferous tubules, and reduction of spermatogenesis. AC also induced neurotoxicity and altered brain neurotransmitter levels. Hemorrhage in the myocardium, disruption of cardiac muscle fibers and pyknotic nuclei in cardiomyocytes were recorded as side effects of AC in heart tissue. Conclusions: The results concluded that AC treatment induced sperm morphological abnormalities and histopathological changes in different body organs. Clinicians must be aware of such results while describing the drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 9 809 816 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Amoxicillin/clavulanic acid Genotoxicity Sperm abnormalities Histopathological changes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Amoxicillin/clavulanic acid Genotoxicity Sperm abnormalities Histopathological changes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Maha A. Fahmy Ayman A. Farghaly Enayat A. Omara Zeinab M. Hassan Fawzia A.E. Aly Souria M. Donya Aziza A.E. Ibrahim Elsayed M. Bayoumy Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
topic_facet |
Amoxicillin/clavulanic acid Genotoxicity Sperm abnormalities Histopathological changes Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To explore the genotoxic potential and histopathological changes induced in liver, kidney, testis, brain and heart after using the antibiotic drug amoxicillin/clavulanic acid (4:1). Methods: The study included chromosomal aberration analysis in bone-marrow and mouse spermatocytes, induction of sperm morphological abnormalities and histopathological changes in different body organs. The drug was administrated orally at a dose of 81 mg/kg body weight twice daily (Total = 162 mg/kg/day) for various periods of time equivalent to 625 mg/men (twice daily). Results: The results revealed non-significant chromosomal aberrations induced after treatment with amoxicillin/clavulanic acid (AC) in both bone marrow and mouse spermatocytes after 7 and 10 days treatment. On the other hand, statistically significant percentages of sperm morphological abnormalities were recorded. Such percentage reached 8.10 ± 0.55, 9.86 ± 0.63 and 12.12 ± 0.58 at the three time intervals tested (7, 14 and 35 days after the 1st treatment respectively) (treatment performed for 5 successive days) compared with 2.78 ± 0.48 for the control. The results also revealed histopathological changes in different body organs after AC treatment which increased with the prolongation of the period of therapy. Congestion of central vain, liver hemorrhage and hydropic changes in hepatocytes were noticed in the liver. Degenerative changes were found in kidney glomerulus and tubules while testis showed atrophy of seminiferous tubules, and reduction of spermatogenesis. AC also induced neurotoxicity and altered brain neurotransmitter levels. Hemorrhage in the myocardium, disruption of cardiac muscle fibers and pyknotic nuclei in cardiomyocytes were recorded as side effects of AC in heart tissue. Conclusions: The results concluded that AC treatment induced sperm morphological abnormalities and histopathological changes in different body organs. Clinicians must be aware of such results while describing the drug. |
format |
Article in Journal/Newspaper |
author |
Maha A. Fahmy Ayman A. Farghaly Enayat A. Omara Zeinab M. Hassan Fawzia A.E. Aly Souria M. Donya Aziza A.E. Ibrahim Elsayed M. Bayoumy |
author_facet |
Maha A. Fahmy Ayman A. Farghaly Enayat A. Omara Zeinab M. Hassan Fawzia A.E. Aly Souria M. Donya Aziza A.E. Ibrahim Elsayed M. Bayoumy |
author_sort |
Maha A. Fahmy |
title |
Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
title_short |
Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
title_full |
Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
title_fullStr |
Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
title_full_unstemmed |
Amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
title_sort |
amoxicillin–clavulanic acid induced sperm abnormalities and histopathological changes in mice |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.08.002 https://doaj.org/article/a417a6015b574996b1786681589fa0c8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 9, Pp 809-816 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169117308390 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.08.002 https://doaj.org/article/a417a6015b574996b1786681589fa0c8 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.08.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
9 |
container_start_page |
809 |
op_container_end_page |
816 |
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1766345743470166016 |