Fishing Innate Immune System Properties through the Transcriptomic Single-Cell Data of Teleostei

• Published in: Biology
• Main Authors: Aleksandr V. Bobrovskikh, Ulyana S. Zubairova, Alexey V. Doroshkov
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2023
• Subjects: aquaculture; drug development; gene networks; immunity; meta-analysis; model organisms; Biology (General); QH301-705.5; Turbot
• Online Access: https://doi.org/10.3390/biology12121516; https://doaj.org/article/a414431911634e6caaf143d017bd2b70

The innate immune system is the first line of defense in multicellular organisms. Danio rerio is widely considered a promising model for IIS-related research, with the most amount of scRNAseq data available among Teleostei . We summarized the scRNAseq and spatial transcriptomics experiments related to the IIS for zebrafish and other Teleostei from the GEO NCBI and the Single-Cell Expression Atlas. We found a considerable number of scRNAseq experiments at different stages of zebrafish development in organs such as the kidney, liver, stomach, heart, and brain. These datasets could be further used to conduct large-scale meta-analyses and to compare the IIS of zebrafish with the mammalian one. However, only a small number of scRNAseq datasets are available for other fish (turbot, salmon, cavefish, and dark sleeper). Since fish biology is very diverse, it would be a major mistake to use zebrafish alone in fish immunology studies. In particular, there is a special need for new scRNAseq experiments involving nonmodel Teleostei , e.g., long-lived species, cancer-resistant fish, and various fish ecotypes.