Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation
Abstract Background Catheter-associated urinary tract infections (CAUTIs) are the most common infections found in healthcare facilities. Urinary catheters predispose the development of CAUTIs by destroying natural barriers and providing a source for infection and biofilm formation (BF). This study a...
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ftdoajarticles:oai:doaj.org/article:a40e18a9bc6f4f18983782a342b0a2c3 2023-05-15T15:14:35+02:00 Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation Mohamed Anwar Mahgoub Aleya Abdel Gawad Abbass Amani Farouk Abaza Mohamed Shafik Shoukry 2023-01-01T00:00:00Z https://doi.org/10.1186/s42506-022-00124-2 https://doaj.org/article/a40e18a9bc6f4f18983782a342b0a2c3 EN eng SpringerOpen https://doi.org/10.1186/s42506-022-00124-2 https://doaj.org/toc/2090-262X doi:10.1186/s42506-022-00124-2 2090-262X https://doaj.org/article/a40e18a9bc6f4f18983782a342b0a2c3 Journal of the Egyptian Public Health Association, Vol 97, Iss 1, Pp 1-9 (2023) Probiotics Catheter-associated urinary tract infections Biofilm Urinary catheter Lactic acid bacteria Lactobacillus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1186/s42506-022-00124-2 2023-01-15T01:29:39Z Abstract Background Catheter-associated urinary tract infections (CAUTIs) are the most common infections found in healthcare facilities. Urinary catheters predispose the development of CAUTIs by destroying natural barriers and providing a source for infection and biofilm formation (BF). This study aimed to evaluate probiotic lactic acid bacteria (LAB) as a means of preventing in vitro urinary catheter colonization and BF. Methods Cross-sectional screening, followed by an experimental study, was conducted on 120 catheterized patients admitted to the urology department in a tertiary care hospital for 7 months. The isolated and identified uropathogens were tested for their antimicrobial susceptibility patterns by the disk diffusion method according to Clinical and Laboratory Standards Institute recommendations and examined for their ability to produce biofilms using a microtiter plate (MtP) assay. Five LAB (Lactobacillus acidophilus (L. acidophilus), Bifidobacterium bifidum (B. bifidum), L. paracasei, L. pentosus, and L. plantarum) were identified and examined for preventing in vitro colonization and BF of some isolated uropathogens on Foley urinary catheter surfaces. Results Of the 120 samples collected, 32.5% were found to be associated with CAUTIs. Of isolated organisms, 74.4% were gram-negative bacilli, while gram-positive cocci represented 14%, and only 11.6% were of the Candida species. About two-thirds of isolated uropathogens were biofilm formers. All five probiotic strains had inhibitory effects on the growth of all the uropathogens tested but with varying intensities according to the duration of application after 2, 4, and 6 days. Conclusions The prevalence of CAUTIs was high, and the predominant bacterial isolates were gram-negative bacilli. Many of the studied uropathogens were biofilm formers. The bacterial isolates had a higher prevalence of resistance to commonly prescribed antimicrobial agents. Probiotics have the potential to prevent in vitro urinary catheter colonization and inhibit BF. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of the Egyptian Public Health Association 97 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Probiotics Catheter-associated urinary tract infections Biofilm Urinary catheter Lactic acid bacteria Lactobacillus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Probiotics Catheter-associated urinary tract infections Biofilm Urinary catheter Lactic acid bacteria Lactobacillus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mohamed Anwar Mahgoub Aleya Abdel Gawad Abbass Amani Farouk Abaza Mohamed Shafik Shoukry Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
topic_facet |
Probiotics Catheter-associated urinary tract infections Biofilm Urinary catheter Lactic acid bacteria Lactobacillus Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Abstract Background Catheter-associated urinary tract infections (CAUTIs) are the most common infections found in healthcare facilities. Urinary catheters predispose the development of CAUTIs by destroying natural barriers and providing a source for infection and biofilm formation (BF). This study aimed to evaluate probiotic lactic acid bacteria (LAB) as a means of preventing in vitro urinary catheter colonization and BF. Methods Cross-sectional screening, followed by an experimental study, was conducted on 120 catheterized patients admitted to the urology department in a tertiary care hospital for 7 months. The isolated and identified uropathogens were tested for their antimicrobial susceptibility patterns by the disk diffusion method according to Clinical and Laboratory Standards Institute recommendations and examined for their ability to produce biofilms using a microtiter plate (MtP) assay. Five LAB (Lactobacillus acidophilus (L. acidophilus), Bifidobacterium bifidum (B. bifidum), L. paracasei, L. pentosus, and L. plantarum) were identified and examined for preventing in vitro colonization and BF of some isolated uropathogens on Foley urinary catheter surfaces. Results Of the 120 samples collected, 32.5% were found to be associated with CAUTIs. Of isolated organisms, 74.4% were gram-negative bacilli, while gram-positive cocci represented 14%, and only 11.6% were of the Candida species. About two-thirds of isolated uropathogens were biofilm formers. All five probiotic strains had inhibitory effects on the growth of all the uropathogens tested but with varying intensities according to the duration of application after 2, 4, and 6 days. Conclusions The prevalence of CAUTIs was high, and the predominant bacterial isolates were gram-negative bacilli. Many of the studied uropathogens were biofilm formers. The bacterial isolates had a higher prevalence of resistance to commonly prescribed antimicrobial agents. Probiotics have the potential to prevent in vitro urinary catheter colonization and inhibit BF. ... |
format |
Article in Journal/Newspaper |
author |
Mohamed Anwar Mahgoub Aleya Abdel Gawad Abbass Amani Farouk Abaza Mohamed Shafik Shoukry |
author_facet |
Mohamed Anwar Mahgoub Aleya Abdel Gawad Abbass Amani Farouk Abaza Mohamed Shafik Shoukry |
author_sort |
Mohamed Anwar Mahgoub |
title |
Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
title_short |
Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
title_full |
Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
title_fullStr |
Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
title_full_unstemmed |
Probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
title_sort |
probiotic lactic acid bacteria as a means of preventing in vitro urinary catheter colonization and biofilm formation |
publisher |
SpringerOpen |
publishDate |
2023 |
url |
https://doi.org/10.1186/s42506-022-00124-2 https://doaj.org/article/a40e18a9bc6f4f18983782a342b0a2c3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of the Egyptian Public Health Association, Vol 97, Iss 1, Pp 1-9 (2023) |
op_relation |
https://doi.org/10.1186/s42506-022-00124-2 https://doaj.org/toc/2090-262X doi:10.1186/s42506-022-00124-2 2090-262X https://doaj.org/article/a40e18a9bc6f4f18983782a342b0a2c3 |
op_doi |
https://doi.org/10.1186/s42506-022-00124-2 |
container_title |
Journal of the Egyptian Public Health Association |
container_volume |
97 |
container_issue |
1 |
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1766345022997790720 |