Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes
Abstract Background Antarctic fishes of the Notothenioidei suborder constitutively upregulate multiple inducible chaperones, a highly derived adaptation that preserves proteostasis in extreme cold, and represent a system for studying the evolution of gene frontloading. We screened for Hsf1-binding s...
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ftdoajarticles:oai:doaj.org/article:a3fcf5dbcec043cdb9913bc625d99168 2023-05-15T14:03:24+02:00 Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes Samuel N. Bogan Sean P. Place 2019-11-01T00:00:00Z https://doi.org/10.1186/s12862-019-1524-y https://doaj.org/article/a3fcf5dbcec043cdb9913bc625d99168 EN eng BMC http://link.springer.com/article/10.1186/s12862-019-1524-y https://doaj.org/toc/1471-2148 doi:10.1186/s12862-019-1524-y 1471-2148 https://doaj.org/article/a3fcf5dbcec043cdb9913bc625d99168 BMC Evolutionary Biology, Vol 19, Iss 1, Pp 1-12 (2019) Comparative genomics Heat shock proteins Notothen Promoters cis-regulation Gene regulation Evolution QH359-425 article 2019 ftdoajarticles https://doi.org/10.1186/s12862-019-1524-y 2022-12-31T06:58:04Z Abstract Background Antarctic fishes of the Notothenioidei suborder constitutively upregulate multiple inducible chaperones, a highly derived adaptation that preserves proteostasis in extreme cold, and represent a system for studying the evolution of gene frontloading. We screened for Hsf1-binding sites, as Hsf1 is a master transcription factor of the heat shock response, and highly-conserved non-coding elements within proximal promoters of chaperone genes across 10 Antarctic notothens, 2 subpolar notothens, and 17 perciform fishes. We employed phylogenetic models of molecular evolution to determine whether (i) changes in motifs associated with Hsf1-binding and/or (ii) relaxed purifying selection or exaptation at ancestral cis-regulatory elements coincided with the evolution of chaperone frontloading in Antarctic notothens. Results Antarctic notothens exhibited significantly fewer Hsf1-binding sites per bp at chaperone promoters than subpolar notothens and Serranoidei, the most closely-related suborder to Notothenioidei included in this study. 90% of chaperone promoters exhibited accelerated substitution rates among Antarctic notothens relative to other perciformes. The proportion of bases undergoing accelerated evolution (i) was significantly greater in Antarctic notothens than in subpolar notothens and Perciformes in 70% of chaperone genes and (ii) increased among bases that were more conserved among perciformes. Lastly, we detected evidence of relaxed purifying selection and exaptation acting on ancestrally conserved cis-regulatory elements in the Antarctic notothen lineage and its major branches. Conclusion A large degree of turnover has occurred in Notothenioidei at chaperone promoter regions that are conserved among perciform fishes following adaptation to the cooling of the Southern Ocean. Additionally, derived reductions in Hsf1-binding site frequency suggest cis-regulatory modifications to the classical heat shock response. Of note, turnover events within chaperone promoters were less frequent in the ... Article in Journal/Newspaper Antarc* Antarctic Southern Ocean Directory of Open Access Journals: DOAJ Articles Antarctic Southern Ocean The Antarctic BMC Evolutionary Biology 19 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Comparative genomics Heat shock proteins Notothen Promoters cis-regulation Gene regulation Evolution QH359-425 |
spellingShingle |
Comparative genomics Heat shock proteins Notothen Promoters cis-regulation Gene regulation Evolution QH359-425 Samuel N. Bogan Sean P. Place Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
topic_facet |
Comparative genomics Heat shock proteins Notothen Promoters cis-regulation Gene regulation Evolution QH359-425 |
description |
Abstract Background Antarctic fishes of the Notothenioidei suborder constitutively upregulate multiple inducible chaperones, a highly derived adaptation that preserves proteostasis in extreme cold, and represent a system for studying the evolution of gene frontloading. We screened for Hsf1-binding sites, as Hsf1 is a master transcription factor of the heat shock response, and highly-conserved non-coding elements within proximal promoters of chaperone genes across 10 Antarctic notothens, 2 subpolar notothens, and 17 perciform fishes. We employed phylogenetic models of molecular evolution to determine whether (i) changes in motifs associated with Hsf1-binding and/or (ii) relaxed purifying selection or exaptation at ancestral cis-regulatory elements coincided with the evolution of chaperone frontloading in Antarctic notothens. Results Antarctic notothens exhibited significantly fewer Hsf1-binding sites per bp at chaperone promoters than subpolar notothens and Serranoidei, the most closely-related suborder to Notothenioidei included in this study. 90% of chaperone promoters exhibited accelerated substitution rates among Antarctic notothens relative to other perciformes. The proportion of bases undergoing accelerated evolution (i) was significantly greater in Antarctic notothens than in subpolar notothens and Perciformes in 70% of chaperone genes and (ii) increased among bases that were more conserved among perciformes. Lastly, we detected evidence of relaxed purifying selection and exaptation acting on ancestrally conserved cis-regulatory elements in the Antarctic notothen lineage and its major branches. Conclusion A large degree of turnover has occurred in Notothenioidei at chaperone promoter regions that are conserved among perciform fishes following adaptation to the cooling of the Southern Ocean. Additionally, derived reductions in Hsf1-binding site frequency suggest cis-regulatory modifications to the classical heat shock response. Of note, turnover events within chaperone promoters were less frequent in the ... |
format |
Article in Journal/Newspaper |
author |
Samuel N. Bogan Sean P. Place |
author_facet |
Samuel N. Bogan Sean P. Place |
author_sort |
Samuel N. Bogan |
title |
Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
title_short |
Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
title_full |
Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
title_fullStr |
Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
title_full_unstemmed |
Accelerated evolution at chaperone promoters among Antarctic notothenioid fishes |
title_sort |
accelerated evolution at chaperone promoters among antarctic notothenioid fishes |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12862-019-1524-y https://doaj.org/article/a3fcf5dbcec043cdb9913bc625d99168 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
BMC Evolutionary Biology, Vol 19, Iss 1, Pp 1-12 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12862-019-1524-y https://doaj.org/toc/1471-2148 doi:10.1186/s12862-019-1524-y 1471-2148 https://doaj.org/article/a3fcf5dbcec043cdb9913bc625d99168 |
op_doi |
https://doi.org/10.1186/s12862-019-1524-y |
container_title |
BMC Evolutionary Biology |
container_volume |
19 |
container_issue |
1 |
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1766274041539198976 |