Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study
Objective: To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice. Methods: GC/MS was used for analysis of active constituents of Calotropis gigantea extract. Spore germina...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2022
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Online Access: | https://doi.org/10.4103/2221-1691.345516 https://doaj.org/article/a3f69e6b404f48cb872d686e0999a046 |
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ftdoajarticles:oai:doaj.org/article:a3f69e6b404f48cb872d686e0999a046 2023-05-15T15:16:20+02:00 Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study Enas M Ali Manal A Alfwuaires Gehan M Badr 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.345516 https://doaj.org/article/a3f69e6b404f48cb872d686e0999a046 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=243;epage=252;aulast=Ali https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.345516 https://doaj.org/article/a3f69e6b404f48cb872d686e0999a046 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 6, Pp 243-252 (2022) aspergillus fumigatus antifungal invasive pulmonary aspergillosis calotropis gigantea amphotericin b mice Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.345516 2022-12-31T03:01:48Z Objective: To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice. Methods: GC/MS was used for analysis of active constituents of Calotropis gigantea extract. Spore germination assay and broth micro-dilution method were used to determine antifungal potential of Calotropis gigantea/amphotericin B against Aspergillus fumigatus. Neutropenic mice were randomly assigned into 5 groups: group 1 was neutropenic (control); group 2 was infected with Aspergillus fumigatus; group 3 was infected with Aspergillus fumigatus, and treated with Calotropis gigantea extract; group 4 was infected with Aspergillus fumigatus and treated with amphotericin B; group 5 was infected with Aspergillus fumigatus and treated with both Calotropis gigantea extract and amphotericin B. Fresh lung tissues were histopathologically examined. Fungal burden and gliotoxin concentration were evaluated in lung tissues. Catalase, superoxide dismutase, and malondialdehyde content were determined in lung tissues. Myeloperoxidase, tumor necrosis factor-alpha, interleukin-1, and interleukin-17 were also estimated by the sandwich enzyme-linked immuno-sorbent assay. Results: Calotropis gigantea/amphotericin B had a minimum inhibitory concentration and minimum fungicidal concentration of 80 and 160 μg/mL, respectively, for Aspergillus fumigatus. Additionally, Calotropis gigantea/amphotericin B significantly reduced lung fungal burden by 72.95% and inhibited production of gliotoxin in lung tissues from 6 320 to 1 350 μg/g lung. Calotropis gigantea/amphotericin B reduced the oxidative stress of the lung via elevating the activity of antioxidant enzymes and decreasing the levels of lipid peroxidation. Myeloperoxidase activity and the production of pro-inflammatory cytokines were also significantly reduced. Scanning electron microscopy revealed deteriorations in the hyphae ultrastructure in Calotropis gigantea/amphotericin B treated ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 6 243 |
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aspergillus fumigatus antifungal invasive pulmonary aspergillosis calotropis gigantea amphotericin b mice Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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aspergillus fumigatus antifungal invasive pulmonary aspergillosis calotropis gigantea amphotericin b mice Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Enas M Ali Manal A Alfwuaires Gehan M Badr Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
topic_facet |
aspergillus fumigatus antifungal invasive pulmonary aspergillosis calotropis gigantea amphotericin b mice Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To characterize the antifungal activity of methanolic leaf extract of Calotropis gigantea alone or in combination with amphotericin B against invasive pulmonary aspergillosis in mice. Methods: GC/MS was used for analysis of active constituents of Calotropis gigantea extract. Spore germination assay and broth micro-dilution method were used to determine antifungal potential of Calotropis gigantea/amphotericin B against Aspergillus fumigatus. Neutropenic mice were randomly assigned into 5 groups: group 1 was neutropenic (control); group 2 was infected with Aspergillus fumigatus; group 3 was infected with Aspergillus fumigatus, and treated with Calotropis gigantea extract; group 4 was infected with Aspergillus fumigatus and treated with amphotericin B; group 5 was infected with Aspergillus fumigatus and treated with both Calotropis gigantea extract and amphotericin B. Fresh lung tissues were histopathologically examined. Fungal burden and gliotoxin concentration were evaluated in lung tissues. Catalase, superoxide dismutase, and malondialdehyde content were determined in lung tissues. Myeloperoxidase, tumor necrosis factor-alpha, interleukin-1, and interleukin-17 were also estimated by the sandwich enzyme-linked immuno-sorbent assay. Results: Calotropis gigantea/amphotericin B had a minimum inhibitory concentration and minimum fungicidal concentration of 80 and 160 μg/mL, respectively, for Aspergillus fumigatus. Additionally, Calotropis gigantea/amphotericin B significantly reduced lung fungal burden by 72.95% and inhibited production of gliotoxin in lung tissues from 6 320 to 1 350 μg/g lung. Calotropis gigantea/amphotericin B reduced the oxidative stress of the lung via elevating the activity of antioxidant enzymes and decreasing the levels of lipid peroxidation. Myeloperoxidase activity and the production of pro-inflammatory cytokines were also significantly reduced. Scanning electron microscopy revealed deteriorations in the hyphae ultrastructure in Calotropis gigantea/amphotericin B treated ... |
format |
Article in Journal/Newspaper |
author |
Enas M Ali Manal A Alfwuaires Gehan M Badr |
author_facet |
Enas M Ali Manal A Alfwuaires Gehan M Badr |
author_sort |
Enas M Ali |
title |
Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
title_short |
Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
title_full |
Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
title_fullStr |
Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
title_full_unstemmed |
Therapeutic potential of Calotropis gigantea extract against invasive pulmonary aspergillosis: In vitro and in vivo study |
title_sort |
therapeutic potential of calotropis gigantea extract against invasive pulmonary aspergillosis: in vitro and in vivo study |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.345516 https://doaj.org/article/a3f69e6b404f48cb872d686e0999a046 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 6, Pp 243-252 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=243;epage=252;aulast=Ali https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.345516 https://doaj.org/article/a3f69e6b404f48cb872d686e0999a046 |
op_doi |
https://doi.org/10.4103/2221-1691.345516 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
6 |
container_start_page |
243 |
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1766346625444216832 |