Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula
The Southern Ocean is currently subject to intense investigations, mainly related to its importance for global biogeochemical cycles and its alarming rate of warming in response to climate change. Microbes play an essential role in the functioning of this ecosystem and are the main drivers of the bi...
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ftdoajarticles:oai:doaj.org/article:a3d33086daf54388a89dd1f8fb89fb1b 2023-05-15T14:02:31+02:00 Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula Camila Negrão Signori François eThomas Alex eEnrich-Prast Ricardo C.G. Pollery Stefan M Sievert 2014-12-01T00:00:00Z https://doi.org/10.3389/fmicb.2014.00647 https://doaj.org/article/a3d33086daf54388a89dd1f8fb89fb1b EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00647/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2014.00647 https://doaj.org/article/a3d33086daf54388a89dd1f8fb89fb1b Frontiers in Microbiology, Vol 5 (2014) Climate Change pyrosequencing environmental factors Microbial Oceanography Antarctica microbial community structure Microbiology QR1-502 article 2014 ftdoajarticles https://doi.org/10.3389/fmicb.2014.00647 2022-12-30T22:39:16Z The Southern Ocean is currently subject to intense investigations, mainly related to its importance for global biogeochemical cycles and its alarming rate of warming in response to climate change. Microbes play an essential role in the functioning of this ecosystem and are the main drivers of the biogeochemical cycling of elements. Yet, the diversity and abundance of microorganisms in this system remains poorly studied, in particular with regards to changes along environmental gradients. Here, we used amplicon sequencing of 16S rRNA gene tags using primers covering both Bacteria and Archaea to assess the composition and diversity of the microbial communities from four sampling depths (surface, the maximum and minimum of the oxygen concentration, and near the seafloor) at ten oceanographic stations located in Bransfield Strait (northwest of the Antarctic Peninsula (AP)) and near the sea ice edge (north of the AP). Samples collected near the seafloor and at the oxygen minimum exhibited a higher diversity than those from the surface and oxygen maximum for both bacterial and archaeal communities. The main taxonomic groups identified below 100 m were Thaumarchaeota, Euryarchaeota and Proteobacteria (Gamma-, Delta-, Beta- and Alphaproteobacteria), whereas in the mixed layer above 100 m Bacteroidetes and Proteobacteria (mainly Alpha- and Gammaproteobacteria) were found to be dominant. A combination of environmental factors seems to influence the microbial community composition. Our results help to understand how the dynamic seascape of the Southern Ocean shapes the microbial community composition and set a baseline for upcoming studies to evaluate the response of this ecosystem to future changes. Article in Journal/Newspaper Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean Directory of Open Access Journals: DOAJ Articles Antarctic Antarctic Peninsula Bransfield Strait Southern Ocean The Antarctic Frontiers in Microbiology 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Climate Change pyrosequencing environmental factors Microbial Oceanography Antarctica microbial community structure Microbiology QR1-502 |
spellingShingle |
Climate Change pyrosequencing environmental factors Microbial Oceanography Antarctica microbial community structure Microbiology QR1-502 Camila Negrão Signori François eThomas Alex eEnrich-Prast Ricardo C.G. Pollery Stefan M Sievert Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
topic_facet |
Climate Change pyrosequencing environmental factors Microbial Oceanography Antarctica microbial community structure Microbiology QR1-502 |
description |
The Southern Ocean is currently subject to intense investigations, mainly related to its importance for global biogeochemical cycles and its alarming rate of warming in response to climate change. Microbes play an essential role in the functioning of this ecosystem and are the main drivers of the biogeochemical cycling of elements. Yet, the diversity and abundance of microorganisms in this system remains poorly studied, in particular with regards to changes along environmental gradients. Here, we used amplicon sequencing of 16S rRNA gene tags using primers covering both Bacteria and Archaea to assess the composition and diversity of the microbial communities from four sampling depths (surface, the maximum and minimum of the oxygen concentration, and near the seafloor) at ten oceanographic stations located in Bransfield Strait (northwest of the Antarctic Peninsula (AP)) and near the sea ice edge (north of the AP). Samples collected near the seafloor and at the oxygen minimum exhibited a higher diversity than those from the surface and oxygen maximum for both bacterial and archaeal communities. The main taxonomic groups identified below 100 m were Thaumarchaeota, Euryarchaeota and Proteobacteria (Gamma-, Delta-, Beta- and Alphaproteobacteria), whereas in the mixed layer above 100 m Bacteroidetes and Proteobacteria (mainly Alpha- and Gammaproteobacteria) were found to be dominant. A combination of environmental factors seems to influence the microbial community composition. Our results help to understand how the dynamic seascape of the Southern Ocean shapes the microbial community composition and set a baseline for upcoming studies to evaluate the response of this ecosystem to future changes. |
format |
Article in Journal/Newspaper |
author |
Camila Negrão Signori François eThomas Alex eEnrich-Prast Ricardo C.G. Pollery Stefan M Sievert |
author_facet |
Camila Negrão Signori François eThomas Alex eEnrich-Prast Ricardo C.G. Pollery Stefan M Sievert |
author_sort |
Camila Negrão Signori |
title |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_short |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_full |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_fullStr |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_full_unstemmed |
Microbial diversity and community structure across environmental gradients in Bransfield Strait, Western Antarctic Peninsula |
title_sort |
microbial diversity and community structure across environmental gradients in bransfield strait, western antarctic peninsula |
publisher |
Frontiers Media S.A. |
publishDate |
2014 |
url |
https://doi.org/10.3389/fmicb.2014.00647 https://doaj.org/article/a3d33086daf54388a89dd1f8fb89fb1b |
geographic |
Antarctic Antarctic Peninsula Bransfield Strait Southern Ocean The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Bransfield Strait Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Bransfield Strait Sea ice Southern Ocean |
op_source |
Frontiers in Microbiology, Vol 5 (2014) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00647/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2014.00647 https://doaj.org/article/a3d33086daf54388a89dd1f8fb89fb1b |
op_doi |
https://doi.org/10.3389/fmicb.2014.00647 |
container_title |
Frontiers in Microbiology |
container_volume |
5 |
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1766272838223790080 |