Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo
The purpose of this study was to evaluate the influence of variables in a flotation technique for the recovery of Toxocara canis eggs from soil. The trials were done under standardized conditions on one gram of previously sterilized soil samples contaminated with 200 eggs of T. canis. The following...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
2009
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Online Access: | https://doi.org/10.1590/S0036-46652009000300007 https://doaj.org/article/a36bbc4743fc45e2ba4fd7de874546c8 |
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ftdoajarticles:oai:doaj.org/article:a36bbc4743fc45e2ba4fd7de874546c8 2024-09-09T19:28:30+00:00 Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo Vamilton Alvares Santarém Luciana Puga Magoti Tathiana Dias Sichieri 2009-06-01T00:00:00Z https://doi.org/10.1590/S0036-46652009000300007 https://doaj.org/article/a36bbc4743fc45e2ba4fd7de874546c8 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652009000300007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652009000300007 0036-4665 1678-9946 https://doaj.org/article/a36bbc4743fc45e2ba4fd7de874546c8 Revista do Instituto de Medicina Tropical de São Paulo, Vol 51, Iss 3, Pp 163-167 (2009) Toxocara canis eggs Recovery technique Soil contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1590/S0036-46652009000300007 2024-08-05T17:49:30Z The purpose of this study was to evaluate the influence of variables in a flotation technique for the recovery of Toxocara canis eggs from soil. The trials were done under standardized conditions on one gram of previously sterilized soil samples contaminated with 200 eggs of T. canis. The following variables were evaluated in serial steps: sieving; type of wash; time of stirring; resuspension of sediment; solution flotation. Centrifuge-flotation in sodium nitrate (d = 1.20 g/cm³) was adopted as an initial technique, using Tween 80 (0.2%) and decinormal sodium hydroxide as solutions for washing the samples. Ten tests were done to compare the variables, using counting in triplicate. The sieving of the material reduced significantly the recovery of eggs (p < 0.001) and the number of eggs recovered was higher when the sediment was resuspended (p < 0.05). After standardization, flotation solutions sodium chloride, zinc sulfate, sodium dichromate, magnesium sulfate, and sodium nitrate (d = 1.20g/cm³) were compared. The best results were obtained by using zinc sulfate solution. In conclusion, the chances of recovering T. canis eggs from samples using flotation solutions can be increased by washing of soil twice using distilled water, and resuspension of sediment. On the other hand, the sieving procedure can drastically reduce the number of eggs. Com o objetivo de avaliar a influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo, amostras de solo foram previamente esterilizadas e divididas em alíquotas de um grama e contaminadas com 200 ovos. Após contaminação, foram comparadas, em etapas seriadas, as variáveis: filtragem, tipo de lavagem e ressuspensão do material. Como ponto de partida, utilizou-se técnica com lavagem de solo em Tween 80 (0,2%) e solução de hidróxido de sódio 0,1N; ressuspensão; e centrífugo-flutuação em solução de nitrato de sódio (d = 1,20 g/cm³). Os ovos recuperados foram contados com 10 repetições e três leituras para cada repetição. A ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Partida ENVELOPE(-61.216,-61.216,-62.589,-62.589) Revista do Instituto de Medicina Tropical de São Paulo 51 3 163 167 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Toxocara canis eggs Recovery technique Soil contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Toxocara canis eggs Recovery technique Soil contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Vamilton Alvares Santarém Luciana Puga Magoti Tathiana Dias Sichieri Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
topic_facet |
Toxocara canis eggs Recovery technique Soil contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The purpose of this study was to evaluate the influence of variables in a flotation technique for the recovery of Toxocara canis eggs from soil. The trials were done under standardized conditions on one gram of previously sterilized soil samples contaminated with 200 eggs of T. canis. The following variables were evaluated in serial steps: sieving; type of wash; time of stirring; resuspension of sediment; solution flotation. Centrifuge-flotation in sodium nitrate (d = 1.20 g/cm³) was adopted as an initial technique, using Tween 80 (0.2%) and decinormal sodium hydroxide as solutions for washing the samples. Ten tests were done to compare the variables, using counting in triplicate. The sieving of the material reduced significantly the recovery of eggs (p < 0.001) and the number of eggs recovered was higher when the sediment was resuspended (p < 0.05). After standardization, flotation solutions sodium chloride, zinc sulfate, sodium dichromate, magnesium sulfate, and sodium nitrate (d = 1.20g/cm³) were compared. The best results were obtained by using zinc sulfate solution. In conclusion, the chances of recovering T. canis eggs from samples using flotation solutions can be increased by washing of soil twice using distilled water, and resuspension of sediment. On the other hand, the sieving procedure can drastically reduce the number of eggs. Com o objetivo de avaliar a influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo, amostras de solo foram previamente esterilizadas e divididas em alíquotas de um grama e contaminadas com 200 ovos. Após contaminação, foram comparadas, em etapas seriadas, as variáveis: filtragem, tipo de lavagem e ressuspensão do material. Como ponto de partida, utilizou-se técnica com lavagem de solo em Tween 80 (0,2%) e solução de hidróxido de sódio 0,1N; ressuspensão; e centrífugo-flutuação em solução de nitrato de sódio (d = 1,20 g/cm³). Os ovos recuperados foram contados com 10 repetições e três leituras para cada repetição. A ... |
format |
Article in Journal/Newspaper |
author |
Vamilton Alvares Santarém Luciana Puga Magoti Tathiana Dias Sichieri |
author_facet |
Vamilton Alvares Santarém Luciana Puga Magoti Tathiana Dias Sichieri |
author_sort |
Vamilton Alvares Santarém |
title |
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
title_short |
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
title_full |
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
title_fullStr |
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
title_full_unstemmed |
Influence of variables on centrifuge-flotation technique for recovery of Toxocara canis eggs from soil Influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de Toxocara canis em solo |
title_sort |
influence of variables on centrifuge-flotation technique for recovery of toxocara canis eggs from soil influência de variáveis na técnica de centrífugo-flutuação para a recuperação de ovos de toxocara canis em solo |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2009 |
url |
https://doi.org/10.1590/S0036-46652009000300007 https://doaj.org/article/a36bbc4743fc45e2ba4fd7de874546c8 |
long_lat |
ENVELOPE(-61.216,-61.216,-62.589,-62.589) |
geographic |
Arctic Partida |
geographic_facet |
Arctic Partida |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 51, Iss 3, Pp 163-167 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652009000300007 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652009000300007 0036-4665 1678-9946 https://doaj.org/article/a36bbc4743fc45e2ba4fd7de874546c8 |
op_doi |
https://doi.org/10.1590/S0036-46652009000300007 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
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51 |
container_issue |
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