Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells
ABSTRACT Background: Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on...
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ftdoajarticles:oai:doaj.org/article:a34f4280341f43d38c517bfe9af0399c 2023-05-15T15:09:39+02:00 Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells Benyamin Shahbazi Zahra Salehi Najafabadi Hamidreza Goudarzi Mahnaz Sajadi Fatemeh Tahoori Masoumeh Bagheri https://doi.org/10.1590/1678-9199-jvatitd-2019-0009 https://doaj.org/article/a34f4280341f43d38c517bfe9af0399c EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0009 https://doaj.org/article/a34f4280341f43d38c517bfe9af0399c Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25 Pseudocerastes persicus venom Persian horned viper HPLC fractions Cytotoxicity Apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0009 2022-12-31T01:54:51Z ABSTRACT Background: Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells. Methods: Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits. Results: The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH release in normal fibroblast cells and high caspase-3 and caspase-9 activities in lung cancer cells. These results indicate that fraction 21 induces apoptosis in cancer cells, whereas necrosis is predominantly caused by cell death in the normal cells. Fraction 21 at the final concentration of 10 μg/mL killed approximately 60% of lung cancer cells, while in normal fibroblast cells very low cell cytotoxic effect was observed. Conclusion: HPLC fraction 21 at low concentrations displayed promising anticancer properties with apoptosis induction in the lung cancer cells. This fraction may, therefore, be considered a promising candidate for further studies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 25 |
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Directory of Open Access Journals: DOAJ Articles |
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Pseudocerastes persicus venom Persian horned viper HPLC fractions Cytotoxicity Apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Pseudocerastes persicus venom Persian horned viper HPLC fractions Cytotoxicity Apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Benyamin Shahbazi Zahra Salehi Najafabadi Hamidreza Goudarzi Mahnaz Sajadi Fatemeh Tahoori Masoumeh Bagheri Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
topic_facet |
Pseudocerastes persicus venom Persian horned viper HPLC fractions Cytotoxicity Apoptosis Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ABSTRACT Background: Several studies have pointed out that certain snake venoms contain compounds presenting cytotoxic activities that selectively interfere with cancer cell metabolism. In this study, Pseudocerastes persicus venom and its fractions were investigated for their anticancer potential on lung cancer cells. Methods: Lung cancer cells (A549) and normal fibroblast cells (Hu02) were treated with the P. persicus venom and its HPLC fractions and the cell cytotoxic effects were analyzed using MTT and lactate dehydrogenase release assays. Apoptosis was determined in venom-treated cell cultures using caspase-3 and caspase-9 assay kits. Results: The treatment of cells with HPLC fraction 21 (25-35 kDa) of P. persicus venom resulted in high LDH release in normal fibroblast cells and high caspase-3 and caspase-9 activities in lung cancer cells. These results indicate that fraction 21 induces apoptosis in cancer cells, whereas necrosis is predominantly caused by cell death in the normal cells. Fraction 21 at the final concentration of 10 μg/mL killed approximately 60% of lung cancer cells, while in normal fibroblast cells very low cell cytotoxic effect was observed. Conclusion: HPLC fraction 21 at low concentrations displayed promising anticancer properties with apoptosis induction in the lung cancer cells. This fraction may, therefore, be considered a promising candidate for further studies. |
format |
Article in Journal/Newspaper |
author |
Benyamin Shahbazi Zahra Salehi Najafabadi Hamidreza Goudarzi Mahnaz Sajadi Fatemeh Tahoori Masoumeh Bagheri |
author_facet |
Benyamin Shahbazi Zahra Salehi Najafabadi Hamidreza Goudarzi Mahnaz Sajadi Fatemeh Tahoori Masoumeh Bagheri |
author_sort |
Benyamin Shahbazi |
title |
Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
title_short |
Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
title_full |
Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
title_fullStr |
Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
title_full_unstemmed |
Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells |
title_sort |
cytotoxic effects of pseudocerastes persicus venom and its hplc fractions on lung cancer cells |
publisher |
SciELO |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0009 https://doaj.org/article/a34f4280341f43d38c517bfe9af0399c |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100319&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0009 https://doaj.org/article/a34f4280341f43d38c517bfe9af0399c |
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https://doi.org/10.1590/1678-9199-jvatitd-2019-0009 |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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25 |
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