Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio.
Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat...
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ftdoajarticles:oai:doaj.org/article:a34cafdba22c4768b5538c63b47cfa6e 2023-05-15T14:03:32+02:00 Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. Maximiliano Estravis-Barcala Katrin Heer Paula Marchelli Birgit Ziegenhagen María Verónica Arana Nicolás Bellora 2021-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0246615 https://doaj.org/article/a34cafdba22c4768b5538c63b47cfa6e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0246615 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0246615 https://doaj.org/article/a34cafdba22c4768b5538c63b47cfa6e PLoS ONE, Vol 16, Iss 3, p e0246615 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1371/journal.pone.0246615 2022-12-30T20:34:25Z Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20°C (control) or 34°C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic PLOS ONE 16 3 e0246615 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Maximiliano Estravis-Barcala Katrin Heer Paula Marchelli Birgit Ziegenhagen María Verónica Arana Nicolás Bellora Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
topic_facet |
Medicine R Science Q |
description |
Global warming is predicted to exert negative impacts on plant growth due to the damaging effect of high temperatures on plant physiology. Revealing the genetic architecture underlying the heat stress response is therefore crucial for the development of conservation strategies, and for breeding heat-resistant plant genotypes. Here we investigated the transcriptional changes induced by heat in Nothofagus pumilio, an emblematic tree species of the sub-Antarctic forests of South America. Through the performance of RNA-seq of leaves of plants exposed to 20°C (control) or 34°C (heat shock), we generated the first transcriptomic resource for the species. We also studied the changes in protein-coding transcripts expression in response to heat. We found 5,214 contigs differentially expressed between temperatures. The heat treatment resulted in a down-regulation of genes related to photosynthesis and carbon metabolism, whereas secondary metabolism, protein re-folding and response to stress were up-regulated. Moreover, several transcription factor families like WRKY or ERF were promoted by heat, alongside spliceosome machinery and hormone signaling pathways. Through a comparative analysis of gene regulation in response to heat in Arabidopsis thaliana, Populus tomentosa and N. pumilio we provide evidence of the existence of shared molecular features of heat stress responses across angiosperms, and identify genes of potential biotechnological application. |
format |
Article in Journal/Newspaper |
author |
Maximiliano Estravis-Barcala Katrin Heer Paula Marchelli Birgit Ziegenhagen María Verónica Arana Nicolás Bellora |
author_facet |
Maximiliano Estravis-Barcala Katrin Heer Paula Marchelli Birgit Ziegenhagen María Verónica Arana Nicolás Bellora |
author_sort |
Maximiliano Estravis-Barcala |
title |
Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
title_short |
Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
title_full |
Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
title_fullStr |
Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
title_full_unstemmed |
Deciphering the transcriptomic regulation of heat stress responses in Nothofagus pumilio. |
title_sort |
deciphering the transcriptomic regulation of heat stress responses in nothofagus pumilio. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pone.0246615 https://doaj.org/article/a34cafdba22c4768b5538c63b47cfa6e |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PLoS ONE, Vol 16, Iss 3, p e0246615 (2021) |
op_relation |
https://doi.org/10.1371/journal.pone.0246615 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0246615 https://doaj.org/article/a34cafdba22c4768b5538c63b47cfa6e |
op_doi |
https://doi.org/10.1371/journal.pone.0246615 |
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PLOS ONE |
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16 |
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3 |
container_start_page |
e0246615 |
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1766274235200700416 |