Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations
In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreat...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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2007
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Online Access: | https://doi.org/10.1590/S1678-91992007000200007 https://doaj.org/article/a34c2e96198c41b39a7139b637a6a561 |
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ftdoajarticles:oai:doaj.org/article:a34c2e96198c41b39a7139b637a6a561 2023-05-15T15:09:22+02:00 Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations L. G. F. Bueno G. B. Leite M. A. Cruz-Höfling L. Rodrigues-Simioni Y. Oshima-Franco 2007-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992007000200007 https://doaj.org/article/a34c2e96198c41b39a7139b637a6a561 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000200007 1678-9199 https://doaj.org/article/a34c2e96198c41b39a7139b637a6a561 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 2, Pp 479-499 (2007) contracture divalent cation Mn2+ myotoxicity neuromuscular blockage neurotoxicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2007 ftdoajarticles https://doi.org/10.1590/S1678-91992007000200007 2022-12-31T00:58:29Z In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu venom myotoxicity. Pretreatment with 1.6mM Mn2+ partially reduced muscle damage percentage and creatine kinase (CK) activity (U/l) induced by both venoms. In conclusion, Mn2+ interfered in ACh-induced contracture of the nicotinic receptor; did not prevent Cdt venom neurotoxicity but partially reduced its myotoxicity in vitro due to the stabilizing action of this venom on the sarcolemmal membrane; and partially attenuated myotoxicity and neuromuscular blockage induced by Bjssu venom. The Mn2+ dual action (pre and postsynaptic) is useful to study snake venoms since most of them present one or both of these actions; besides, Mn2+ allowed recovering coherent interpretation of experimental versus clinical results. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Krebs ENVELOPE(-61.467,-61.467,-64.633,-64.633) Journal of Venomous Animals and Toxins including Tropical Diseases 13 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
contracture divalent cation Mn2+ myotoxicity neuromuscular blockage neurotoxicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
contracture divalent cation Mn2+ myotoxicity neuromuscular blockage neurotoxicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 L. G. F. Bueno G. B. Leite M. A. Cruz-Höfling L. Rodrigues-Simioni Y. Oshima-Franco Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
topic_facet |
contracture divalent cation Mn2+ myotoxicity neuromuscular blockage neurotoxicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
In the present study, manganese (Mn2+), a neuromuscular blocker with pre and postsynaptic actions, was used to verify the neurotoxicity and myotoxicity induced by Crotalus durissus terrificus (Cdt) and Bothrops jararacussu (Bjssu) venoms in biventer cervicis preparations (BCp). Preparations pretreated with 0.66 and 1.6mM Mn2+ did not affect Cdt venom-induced blockage nor change KCl-induced contracture but partially reduced ACh-induced contracture. However, both Mn2+ concentrations partially hindered Bjssu venom-induced blockage after washing the preparations with Krebs solution, and only 1.6mM Mn2+ preparations significantly recovered ACh-induced contracture. The effect of Cdt venom myotoxicity on contractile responses was different from that of Bjssu venom myotoxicity. Pretreatment with 1.6mM Mn2+ partially reduced muscle damage percentage and creatine kinase (CK) activity (U/l) induced by both venoms. In conclusion, Mn2+ interfered in ACh-induced contracture of the nicotinic receptor; did not prevent Cdt venom neurotoxicity but partially reduced its myotoxicity in vitro due to the stabilizing action of this venom on the sarcolemmal membrane; and partially attenuated myotoxicity and neuromuscular blockage induced by Bjssu venom. The Mn2+ dual action (pre and postsynaptic) is useful to study snake venoms since most of them present one or both of these actions; besides, Mn2+ allowed recovering coherent interpretation of experimental versus clinical results. |
format |
Article in Journal/Newspaper |
author |
L. G. F. Bueno G. B. Leite M. A. Cruz-Höfling L. Rodrigues-Simioni Y. Oshima-Franco |
author_facet |
L. G. F. Bueno G. B. Leite M. A. Cruz-Höfling L. Rodrigues-Simioni Y. Oshima-Franco |
author_sort |
L. G. F. Bueno |
title |
Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
title_short |
Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
title_full |
Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
title_fullStr |
Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
title_full_unstemmed |
Effects of manganese (Mn2+) on neurotoxic and myotoxic activities induced by Crotalus durissus terrificus and Bothrops jararacussu venoms in chick biventer cervicis preparations |
title_sort |
effects of manganese (mn2+) on neurotoxic and myotoxic activities induced by crotalus durissus terrificus and bothrops jararacussu venoms in chick biventer cervicis preparations |
publisher |
SciELO |
publishDate |
2007 |
url |
https://doi.org/10.1590/S1678-91992007000200007 https://doaj.org/article/a34c2e96198c41b39a7139b637a6a561 |
long_lat |
ENVELOPE(-61.467,-61.467,-64.633,-64.633) |
geographic |
Arctic Krebs |
geographic_facet |
Arctic Krebs |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 13, Iss 2, Pp 479-499 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200007 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992007000200007 1678-9199 https://doaj.org/article/a34c2e96198c41b39a7139b637a6a561 |
op_doi |
https://doi.org/10.1590/S1678-91992007000200007 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
13 |
container_issue |
2 |
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1766340578060009472 |