Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria
Abstract Background Accurate diagnosis of malaria is an essential prerequisite for proper treatment and drug resistance monitoring. Microscopy is considered the gold standard for malaria diagnosis but has limitations. ELISA, PCR, and Real Time PCR are also used to diagnose malaria in reference labor...
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ftdoajarticles:oai:doaj.org/article:a33e437dc38c4313acc05b742d65d82b 2023-05-15T15:13:26+02:00 Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria Mohon Abu Naser Elahi Rubayet Podder Milka Patracia Mohiuddin Khaja Hossain Mohammad Sharif Khan Wasif A Haque Rashidul Alam Mohammad Shafiul 2012-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-415 https://doaj.org/article/a33e437dc38c4313acc05b742d65d82b EN eng BMC http://www.malariajournal.com/content/11/1/415 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-415 1475-2875 https://doaj.org/article/a33e437dc38c4313acc05b742d65d82b Malaria Journal, Vol 11, Iss 1, p 415 (2012) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-415 2022-12-31T12:09:54Z Abstract Background Accurate diagnosis of malaria is an essential prerequisite for proper treatment and drug resistance monitoring. Microscopy is considered the gold standard for malaria diagnosis but has limitations. ELISA, PCR, and Real Time PCR are also used to diagnose malaria in reference laboratories, although their application at the field level is currently not feasible. Rapid diagnostic tests (RDTs) however, have been brought into field operation and widely adopted in recent days. This study evaluates OnSite (Pf/Pan) antigen test, a new RDT introduced by CTK Biotech Inc, USA for malaria diagnosis in a reference setting. Methods Blood samples were collected from febrile patients referred for malaria diagnosis by clinicians. Subjects were included in this study from two different Upazila Health Complexes (UHCs) situated in two malaria endemic districts of Bangladesh. Microscopy and nested PCR were considered the gold standard in this study. OnSite (Pf/Pan) RDT was performed on preserved whole blood samples. Results In total, 372 febrile subjects were included in this study. Of these subjects, 229 (61.6%) tested positive for Plasmodium infection detected by microscopy and nested PCR. OnSite (Pf/Pan) RDT was 94.2% sensitive (95% CI, 89.3-97.3) and 99.5% specific (95% CI, 97.4-00.0) for Plasmodium falciparum diagnosis and 97.3% sensitive (95% CI, 90.5-99.7) and 98.7% specific (95% CI, 96.6-99.6) for Plasmodium vivax diagnosis. Sensitivity varied with differential parasite count for both P. falciparum and P. vivax . The highest sensitivity was observed in febrile patients with parasitaemia that ranged from 501–1,000 parasites/μL regardless of the Plasmodium species. Conclusion The new OnSite (Pf/Pan) RDT is both sensitive and specific for symptomatic malaria diagnosis in standard laboratory conditions. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 415 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohon Abu Naser Elahi Rubayet Podder Milka Patracia Mohiuddin Khaja Hossain Mohammad Sharif Khan Wasif A Haque Rashidul Alam Mohammad Shafiul Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Accurate diagnosis of malaria is an essential prerequisite for proper treatment and drug resistance monitoring. Microscopy is considered the gold standard for malaria diagnosis but has limitations. ELISA, PCR, and Real Time PCR are also used to diagnose malaria in reference laboratories, although their application at the field level is currently not feasible. Rapid diagnostic tests (RDTs) however, have been brought into field operation and widely adopted in recent days. This study evaluates OnSite (Pf/Pan) antigen test, a new RDT introduced by CTK Biotech Inc, USA for malaria diagnosis in a reference setting. Methods Blood samples were collected from febrile patients referred for malaria diagnosis by clinicians. Subjects were included in this study from two different Upazila Health Complexes (UHCs) situated in two malaria endemic districts of Bangladesh. Microscopy and nested PCR were considered the gold standard in this study. OnSite (Pf/Pan) RDT was performed on preserved whole blood samples. Results In total, 372 febrile subjects were included in this study. Of these subjects, 229 (61.6%) tested positive for Plasmodium infection detected by microscopy and nested PCR. OnSite (Pf/Pan) RDT was 94.2% sensitive (95% CI, 89.3-97.3) and 99.5% specific (95% CI, 97.4-00.0) for Plasmodium falciparum diagnosis and 97.3% sensitive (95% CI, 90.5-99.7) and 98.7% specific (95% CI, 96.6-99.6) for Plasmodium vivax diagnosis. Sensitivity varied with differential parasite count for both P. falciparum and P. vivax . The highest sensitivity was observed in febrile patients with parasitaemia that ranged from 501–1,000 parasites/μL regardless of the Plasmodium species. Conclusion The new OnSite (Pf/Pan) RDT is both sensitive and specific for symptomatic malaria diagnosis in standard laboratory conditions. |
format |
Article in Journal/Newspaper |
author |
Mohon Abu Naser Elahi Rubayet Podder Milka Patracia Mohiuddin Khaja Hossain Mohammad Sharif Khan Wasif A Haque Rashidul Alam Mohammad Shafiul |
author_facet |
Mohon Abu Naser Elahi Rubayet Podder Milka Patracia Mohiuddin Khaja Hossain Mohammad Sharif Khan Wasif A Haque Rashidul Alam Mohammad Shafiul |
author_sort |
Mohon Abu Naser |
title |
Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
title_short |
Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
title_full |
Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
title_fullStr |
Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
title_full_unstemmed |
Evaluation of the OnSite (Pf/Pan) rapid diagnostic test for diagnosis of clinical malaria |
title_sort |
evaluation of the onsite (pf/pan) rapid diagnostic test for diagnosis of clinical malaria |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-415 https://doaj.org/article/a33e437dc38c4313acc05b742d65d82b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 415 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/415 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-415 1475-2875 https://doaj.org/article/a33e437dc38c4313acc05b742d65d82b |
op_doi |
https://doi.org/10.1186/1475-2875-11-415 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
415 |
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1766343991318544384 |