BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation
ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer...
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ftdoajarticles:oai:doaj.org/article:a33212e1e1554344ba55c80b25a675c4 2023-05-15T15:09:52+02:00 BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation Patrícia Heloise Alves Bezerra Isadora Marques Ferreira Beatriz Tinoco Franceschi Francine Bianchini Luciana Ambrósio Adélia Cristina O. Cintra Suely Vilela Sampaio Fabíola Attié de Castro Maria Regina Torqueti https://doi.org/10.1590/1678-9199-jvatitd-2019-0010 https://doaj.org/article/a33212e1e1554344ba55c80b25a675c4 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100315&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0010 https://doaj.org/article/a33212e1e1554344ba55c80b25a675c4 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25 apoptosis bothropstoxin breast cancer cancer stem cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0010 2022-12-31T10:55:26Z ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines. Methods: BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide. Results: BthTX-I at 102 µg/mL induced cell death in all cell lines. The toxin induced apoptosis in MCF7, SKBR3, and MDAMB231 in a dose-dependent manner, as confirmed by the increasing number of hypodiploid nuclei. Expression of pro-caspase 3, pro-caspase 8 and Beclin-1 proteins were increased, while the level of the antiapoptotic protein Bcl-2 was diminished in MCF7 cells. BthTX-I changed the staining pattern of CSCs in MDAMB231 cells by increasing expression of CD24 receptors, which mediated cell death. Conclusions: BthTX-I induces apoptosis and autophagy in all breast cancer cell lines tested and also reduces CSCs subpopulation, which makes it a promising therapeutic alternative for breast cancer. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 25 |
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Directory of Open Access Journals: DOAJ Articles |
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apoptosis bothropstoxin breast cancer cancer stem cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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apoptosis bothropstoxin breast cancer cancer stem cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Patrícia Heloise Alves Bezerra Isadora Marques Ferreira Beatriz Tinoco Franceschi Francine Bianchini Luciana Ambrósio Adélia Cristina O. Cintra Suely Vilela Sampaio Fabíola Attié de Castro Maria Regina Torqueti BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
topic_facet |
apoptosis bothropstoxin breast cancer cancer stem cells Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
ABSTRACT Background: Breast cancer is the neoplasm with both the highest incidence and mortality rate among women worldwide. Given the known snake venom cytotoxicity towards several tumor types, we evaluated the effects of BthTX-I from Bothrops jararacussu on MCF7, SKBR3, and MDAMB231 breast cancer cell lines. Methods: BthTX-I cytotoxicity was determined via MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide assay. Cell death was measured by a hypotonic fluorescent solution method, annexin-V-FITC/propidium iodide staining and by apoptotic/autophagic protein expression. Cancer stem cells (CSCs) were quantified by flow cytometry using anti-CD24-FITC and anti-CD44-APC antibodies and propidium iodide. Results: BthTX-I at 102 µg/mL induced cell death in all cell lines. The toxin induced apoptosis in MCF7, SKBR3, and MDAMB231 in a dose-dependent manner, as confirmed by the increasing number of hypodiploid nuclei. Expression of pro-caspase 3, pro-caspase 8 and Beclin-1 proteins were increased, while the level of the antiapoptotic protein Bcl-2 was diminished in MCF7 cells. BthTX-I changed the staining pattern of CSCs in MDAMB231 cells by increasing expression of CD24 receptors, which mediated cell death. Conclusions: BthTX-I induces apoptosis and autophagy in all breast cancer cell lines tested and also reduces CSCs subpopulation, which makes it a promising therapeutic alternative for breast cancer. |
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Article in Journal/Newspaper |
author |
Patrícia Heloise Alves Bezerra Isadora Marques Ferreira Beatriz Tinoco Franceschi Francine Bianchini Luciana Ambrósio Adélia Cristina O. Cintra Suely Vilela Sampaio Fabíola Attié de Castro Maria Regina Torqueti |
author_facet |
Patrícia Heloise Alves Bezerra Isadora Marques Ferreira Beatriz Tinoco Franceschi Francine Bianchini Luciana Ambrósio Adélia Cristina O. Cintra Suely Vilela Sampaio Fabíola Attié de Castro Maria Regina Torqueti |
author_sort |
Patrícia Heloise Alves Bezerra |
title |
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
title_short |
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
title_full |
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
title_fullStr |
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
title_full_unstemmed |
BthTX-I from Bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
title_sort |
bthtx-i from bothrops jararacussu induces apoptosis in human breast cancer cell lines and decreases cancer stem cell subpopulation |
publisher |
SciELO |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0010 https://doaj.org/article/a33212e1e1554344ba55c80b25a675c4 |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 25 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100315&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0010 https://doaj.org/article/a33212e1e1554344ba55c80b25a675c4 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0010 |
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Journal of Venomous Animals and Toxins including Tropical Diseases |
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25 |
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1766340965783568384 |