Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection.
Hemorrhagic fever outbreaks are difficult to diagnose and control in part because of a lack of low-cost and easily accessible diagnostic structures in countries where etiologic agents are present. Furthermore, initial clinical symptoms are common and shared with other endemic diseases such as malari...
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ftdoajarticles:oai:doaj.org/article:a32d69d0fa0244148a115c6b8ed277fd 2023-05-15T15:14:48+02:00 Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. Céline Couturier Atsuhiko Wada Karen Louis Maxime Mistretta Benoit Beitz Moriba Povogui Maryline Ripaux Charlotte Mignon Bettina Werle Adrien Lugari Delphine Pannetier Sabine Godard Anne Bocquin Stéphane Mely Ismaël Béavogui Jean Hébélamou David Leuenberger Philippe Leissner Takeshi Yamamoto Patrick Lécine Christophe Védrine Julie Chaix 2020-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007965 https://doaj.org/article/a32d69d0fa0244148a115c6b8ed277fd EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007965 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007965 https://doaj.org/article/a32d69d0fa0244148a115c6b8ed277fd PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0007965 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007965 2022-12-31T13:17:57Z Hemorrhagic fever outbreaks are difficult to diagnose and control in part because of a lack of low-cost and easily accessible diagnostic structures in countries where etiologic agents are present. Furthermore, initial clinical symptoms are common and shared with other endemic diseases such as malaria or typhoid fever. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need, particularly in outbreak settings. Therefore, rapid diagnostic tests such as lateral flow can be broadly deployed and are typically well-suited to rapidly diagnose hemorrhagic fever viruses, such as Ebola virus. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect very low amount of virus in blood. Here, we developed and characterized an immunoassay test based on immunochromatography coupled to silver amplification technology to detect the secreted glycoprotein of EBOV. The glycoprotein is among the first viral proteins to be detected in blood. This strategy aims at identifying infected patients early following onset of symptoms by detecting low amount of sGP protein in blood samples. The limit of detection achieved by this sGP-targeted kit is 2.2 x 104 genome copies/ml in plasma as assayed in a monkey analytical cohort. Clinical performance evaluation showed a specificity of 100% and a sensitivity of 85.7% when evaluated with plasma samples from healthy controls and patients infected with Zaire Ebola virus from Macenta, Guinea. This rapid and accurate diagnostic test could therefore be used in endemic countries for early detection of infected individuals in point of care settings. Moreover, it could also support efficient clinical triage in hospitals or clinical centers and thus reducing transmission rates to prevent and better manage future severe outbreaks. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 1 e0007965 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Céline Couturier Atsuhiko Wada Karen Louis Maxime Mistretta Benoit Beitz Moriba Povogui Maryline Ripaux Charlotte Mignon Bettina Werle Adrien Lugari Delphine Pannetier Sabine Godard Anne Bocquin Stéphane Mely Ismaël Béavogui Jean Hébélamou David Leuenberger Philippe Leissner Takeshi Yamamoto Patrick Lécine Christophe Védrine Julie Chaix Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Hemorrhagic fever outbreaks are difficult to diagnose and control in part because of a lack of low-cost and easily accessible diagnostic structures in countries where etiologic agents are present. Furthermore, initial clinical symptoms are common and shared with other endemic diseases such as malaria or typhoid fever. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need, particularly in outbreak settings. Therefore, rapid diagnostic tests such as lateral flow can be broadly deployed and are typically well-suited to rapidly diagnose hemorrhagic fever viruses, such as Ebola virus. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect very low amount of virus in blood. Here, we developed and characterized an immunoassay test based on immunochromatography coupled to silver amplification technology to detect the secreted glycoprotein of EBOV. The glycoprotein is among the first viral proteins to be detected in blood. This strategy aims at identifying infected patients early following onset of symptoms by detecting low amount of sGP protein in blood samples. The limit of detection achieved by this sGP-targeted kit is 2.2 x 104 genome copies/ml in plasma as assayed in a monkey analytical cohort. Clinical performance evaluation showed a specificity of 100% and a sensitivity of 85.7% when evaluated with plasma samples from healthy controls and patients infected with Zaire Ebola virus from Macenta, Guinea. This rapid and accurate diagnostic test could therefore be used in endemic countries for early detection of infected individuals in point of care settings. Moreover, it could also support efficient clinical triage in hospitals or clinical centers and thus reducing transmission rates to prevent and better manage future severe outbreaks. |
format |
Article in Journal/Newspaper |
author |
Céline Couturier Atsuhiko Wada Karen Louis Maxime Mistretta Benoit Beitz Moriba Povogui Maryline Ripaux Charlotte Mignon Bettina Werle Adrien Lugari Delphine Pannetier Sabine Godard Anne Bocquin Stéphane Mely Ismaël Béavogui Jean Hébélamou David Leuenberger Philippe Leissner Takeshi Yamamoto Patrick Lécine Christophe Védrine Julie Chaix |
author_facet |
Céline Couturier Atsuhiko Wada Karen Louis Maxime Mistretta Benoit Beitz Moriba Povogui Maryline Ripaux Charlotte Mignon Bettina Werle Adrien Lugari Delphine Pannetier Sabine Godard Anne Bocquin Stéphane Mely Ismaël Béavogui Jean Hébélamou David Leuenberger Philippe Leissner Takeshi Yamamoto Patrick Lécine Christophe Védrine Julie Chaix |
author_sort |
Céline Couturier |
title |
Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
title_short |
Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
title_full |
Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
title_fullStr |
Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
title_full_unstemmed |
Characterization and analytical validation of a new antigenic rapid diagnostic test for Ebola virus disease detection. |
title_sort |
characterization and analytical validation of a new antigenic rapid diagnostic test for ebola virus disease detection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0007965 https://doaj.org/article/a32d69d0fa0244148a115c6b8ed277fd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0007965 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007965 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007965 https://doaj.org/article/a32d69d0fa0244148a115c6b8ed277fd |
op_doi |
https://doi.org/10.1371/journal.pntd.0007965 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
1 |
container_start_page |
e0007965 |
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1766345214987862016 |