An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope.
BACKGROUND:Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. METHODOLOGY/PRINCIPAL FINDINGS:To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chime...
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Online Access: | https://doi.org/10.1371/journal.pntd.0006693 https://doaj.org/article/a2c9ded61f2a4fa1a4fbaed72ca3e21e |
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ftdoajarticles:oai:doaj.org/article:a2c9ded61f2a4fa1a4fbaed72ca3e21e 2023-05-15T15:01:19+02:00 An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. Jing Jin Michael B Sherman Daniel Chafets Nuntana Dinglasan Kai Lu Tzong-Hae Lee Lars-Anders Carlson Marcus O Muench Graham Simmons 2018-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006693 https://doaj.org/article/a2c9ded61f2a4fa1a4fbaed72ca3e21e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6086482?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006693 https://doaj.org/article/a2c9ded61f2a4fa1a4fbaed72ca3e21e PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006693 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006693 2022-12-31T11:11:42Z BACKGROUND:Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. METHODOLOGY/PRINCIPAL FINDINGS:To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. CONCLUSIONS/SIGNIFICANCE:Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 7 e0006693 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jing Jin Michael B Sherman Daniel Chafets Nuntana Dinglasan Kai Lu Tzong-Hae Lee Lars-Anders Carlson Marcus O Muench Graham Simmons An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Chikungunya virus (CHIKV) is the most common alphavirus infecting humans worldwide, causing acute and chronically debilitating arthralgia at a great economic expense. METHODOLOGY/PRINCIPAL FINDINGS:To facilitate our study of CHIKV, we generated a mCherry tagged replication-competent chimeric virus, CHIKV 37997-mCherry. Single particle cryoEM demonstrated icosahedral organization of the chimeric virus and the display of mCherry proteins on virus surface. CHIKV 37997-mCherry is attenuated in both IFNαR knockout and wild-type mice. Strong anti-CHIKV and anti-mCherry antibody responses were induced in CHIKV 37997-mCherry infected mice. CONCLUSIONS/SIGNIFICANCE:Our work suggests that chimeric alphaviruses displaying foreign antigen can serve as vaccines against both aphaviruses and other pathogens and diseases. |
format |
Article in Journal/Newspaper |
author |
Jing Jin Michael B Sherman Daniel Chafets Nuntana Dinglasan Kai Lu Tzong-Hae Lee Lars-Anders Carlson Marcus O Muench Graham Simmons |
author_facet |
Jing Jin Michael B Sherman Daniel Chafets Nuntana Dinglasan Kai Lu Tzong-Hae Lee Lars-Anders Carlson Marcus O Muench Graham Simmons |
author_sort |
Jing Jin |
title |
An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
title_short |
An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
title_full |
An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
title_fullStr |
An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
title_full_unstemmed |
An attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
title_sort |
attenuated replication-competent chikungunya virus with a fluorescently tagged envelope. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006693 https://doaj.org/article/a2c9ded61f2a4fa1a4fbaed72ca3e21e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 7, p e0006693 (2018) |
op_relation |
http://europepmc.org/articles/PMC6086482?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006693 https://doaj.org/article/a2c9ded61f2a4fa1a4fbaed72ca3e21e |
op_doi |
https://doi.org/10.1371/journal.pntd.0006693 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
7 |
container_start_page |
e0006693 |
_version_ |
1766333338443841536 |