Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas
BackgroundLysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in he...
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ftdoajarticles:oai:doaj.org/article:a2ae6def95f04b62a8fe095c9320cdec 2023-05-15T15:58:25+02:00 Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas Xiaoyu Gu Xue Qiao Simiao Yu Xiaorui Song Lingling Wang Linsheng Song 2022-12-01T00:00:00Z https://doi.org/10.3389/fimmu.2022.1088149 https://doaj.org/article/a2ae6def95f04b62a8fe095c9320cdec EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2022.1088149/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2022.1088149 https://doaj.org/article/a2ae6def95f04b62a8fe095c9320cdec Frontiers in Immunology, Vol 13 (2022) Crassostrea gigas hemocytes LSD1 histone demethylation cell proliferation Immunologic diseases. Allergy RC581-607 article 2022 ftdoajarticles https://doi.org/10.3389/fimmu.2022.1088149 2022-12-30T22:27:07Z BackgroundLysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in hematopoiesis and embryonic development process. Here, a conserved LSD1 (CgLSD1) with a SWIRM domain and an amino oxidase (AO) domain was identified from the Pacific oyster Crassostrea gigas.MethodsWe conducted a comprehensive analysis by various means to verify the function of CgLSD1 in hematopoietic process, including quantitative real-time PCR (qRT-PCR) analysis, western blot analysis, immunofluorescence assay, RNA interference (RNAi) and flow cytometry.ResultsThe qRT-PCR analysis revealed that the transcripts of CgLSD1 were widely expressed in oyster tissues with the highest level in the mantle. And the transcripts of CgLSD1 were ubiquitously expressed during larval development with the highest expression level at the early D-veliger larvae stage. In hemocytes after Vibrio splendidus stimulation, the transcripts of CgLSD1 were significantly downregulated at 3, 6, 24, and 48 h with the lowest level at 3 h compared to that in the Seawater group (SW group). Immunocytochemical analysis showed that CgLSD1 was mainly distributed in the nucleus of hemocytes. After the CgLSD1 was knocked down by RNAi, the H3K4me1 and H3K4me2 methylation level significantly increased in hemocyte protein. Besides, the percentage of hemocytes with EdU-positive signals in the total circulating hemocytes significantly increased after V. splendidus stimulation. After RNAi of CgLSD1, the expression of potential granulocyte markers CgSOX11 and CgAATase as well as oyster cytokine-like factor CgAstakine were increased significantly in mRNA level, while the transcripts of potential agranulocyte marker CgCD9 was decreased significantly after V. splendidus stimulation.ConclusionThe above results demonstrated that CgLSD1 was a conserved member of lysine ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Immunology 13 |
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English |
topic |
Crassostrea gigas hemocytes LSD1 histone demethylation cell proliferation Immunologic diseases. Allergy RC581-607 |
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Crassostrea gigas hemocytes LSD1 histone demethylation cell proliferation Immunologic diseases. Allergy RC581-607 Xiaoyu Gu Xue Qiao Simiao Yu Xiaorui Song Lingling Wang Linsheng Song Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas hemocytes LSD1 histone demethylation cell proliferation Immunologic diseases. Allergy RC581-607 |
description |
BackgroundLysine-specific demethylase 1 (LSD1) is an essential epigenetic regulator of hematopoietic differentiation, which can specifically mono-methylate H3K4 (H3K4me1) and di-methylate H3K4 (H3K4me2) as a transcriptional corepressor. Previous reports have been suggested that it participated in hematopoiesis and embryonic development process. Here, a conserved LSD1 (CgLSD1) with a SWIRM domain and an amino oxidase (AO) domain was identified from the Pacific oyster Crassostrea gigas.MethodsWe conducted a comprehensive analysis by various means to verify the function of CgLSD1 in hematopoietic process, including quantitative real-time PCR (qRT-PCR) analysis, western blot analysis, immunofluorescence assay, RNA interference (RNAi) and flow cytometry.ResultsThe qRT-PCR analysis revealed that the transcripts of CgLSD1 were widely expressed in oyster tissues with the highest level in the mantle. And the transcripts of CgLSD1 were ubiquitously expressed during larval development with the highest expression level at the early D-veliger larvae stage. In hemocytes after Vibrio splendidus stimulation, the transcripts of CgLSD1 were significantly downregulated at 3, 6, 24, and 48 h with the lowest level at 3 h compared to that in the Seawater group (SW group). Immunocytochemical analysis showed that CgLSD1 was mainly distributed in the nucleus of hemocytes. After the CgLSD1 was knocked down by RNAi, the H3K4me1 and H3K4me2 methylation level significantly increased in hemocyte protein. Besides, the percentage of hemocytes with EdU-positive signals in the total circulating hemocytes significantly increased after V. splendidus stimulation. After RNAi of CgLSD1, the expression of potential granulocyte markers CgSOX11 and CgAATase as well as oyster cytokine-like factor CgAstakine were increased significantly in mRNA level, while the transcripts of potential agranulocyte marker CgCD9 was decreased significantly after V. splendidus stimulation.ConclusionThe above results demonstrated that CgLSD1 was a conserved member of lysine ... |
format |
Article in Journal/Newspaper |
author |
Xiaoyu Gu Xue Qiao Simiao Yu Xiaorui Song Lingling Wang Linsheng Song |
author_facet |
Xiaoyu Gu Xue Qiao Simiao Yu Xiaorui Song Lingling Wang Linsheng Song |
author_sort |
Xiaoyu Gu |
title |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_short |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_full |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_fullStr |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_full_unstemmed |
Histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster Crassostrea gigas |
title_sort |
histone lysine-specific demethylase 1 regulates the proliferation of hemocytes in the oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fimmu.2022.1088149 https://doaj.org/article/a2ae6def95f04b62a8fe095c9320cdec |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Immunology, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2022.1088149/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2022.1088149 https://doaj.org/article/a2ae6def95f04b62a8fe095c9320cdec |
op_doi |
https://doi.org/10.3389/fimmu.2022.1088149 |
container_title |
Frontiers in Immunology |
container_volume |
13 |
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1766394168414830592 |