Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae)
Objective: To assess the antioxidant activity of Ficus pseudopalma Blanco (Moraceae) (F. pseudopalma) and characterize the active components present in it. Methods: Column chromatography of crude ethanol leaf extract of F. pseudopalma was performed and seven fractions were obtained, labeled as F1, F...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2014
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ftdoajarticles:oai:doaj.org/article:a2ae2c827b144e0988190fe9bc73c477 2023-05-15T15:11:39+02:00 Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) Librado A Santiago Anna Beatriz R Mayor 2014-02-01T00:00:00Z https://doi.org/10.1016/S2221-1691(14)60218-5 https://doaj.org/article/a2ae2c827b144e0988190fe9bc73c477 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115301799 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60218-5 https://doaj.org/article/a2ae2c827b144e0988190fe9bc73c477 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 2, Pp 109-118 (2014) Antioxidant Oxidative stress Free radical scavenging Reducing power Phenolic acids Ficus pseudopalma Blanco Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.1016/S2221-1691(14)60218-5 2022-12-31T01:59:31Z Objective: To assess the antioxidant activity of Ficus pseudopalma Blanco (Moraceae) (F. pseudopalma) and characterize the active components present in it. Methods: Column chromatography of crude ethanol leaf extract of F. pseudopalma was performed and seven fractions were obtained, labeled as F1, F2, F3, F4, F5, F6, F7. DPPH, FRAP, Griess, Fenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions. Thin layer chromatography (TLC), high performance liquid chromatography and Fourier transfer infrared spectroscopy (FTIR) were performed to identify and characterize the bioactive component present in each fractions of F. pseudopalma. Results: DPPH and FRAP assay showed that F5, F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions. All fractions exhibited a good nitric oxide radical scavenging activity wherein F1, F2 and F3 showed the highest inhibition. However, all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals. Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions. Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6. Infrared spectra of the fractions revealed the presence of C-C, OH, aromatic C=C and C=O groups. Conclusions: The identified lupeol in F. pseudopalma may be responsible for the exhibited antioxidant property of the plant. Furthermore, knowing the antioxidant capability of the plant, F. pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Blanco ENVELOPE(-55.233,-55.233,-61.250,-61.250) Fenton ENVELOPE(161.917,161.917,-74.333,-74.333) Asian Pacific Journal of Tropical Biomedicine 4 2 109 118 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antioxidant Oxidative stress Free radical scavenging Reducing power Phenolic acids Ficus pseudopalma Blanco Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Antioxidant Oxidative stress Free radical scavenging Reducing power Phenolic acids Ficus pseudopalma Blanco Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Librado A Santiago Anna Beatriz R Mayor Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
topic_facet |
Antioxidant Oxidative stress Free radical scavenging Reducing power Phenolic acids Ficus pseudopalma Blanco Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To assess the antioxidant activity of Ficus pseudopalma Blanco (Moraceae) (F. pseudopalma) and characterize the active components present in it. Methods: Column chromatography of crude ethanol leaf extract of F. pseudopalma was performed and seven fractions were obtained, labeled as F1, F2, F3, F4, F5, F6, F7. DPPH, FRAP, Griess, Fenton and superoxide radical scavenging assays were performed to assess the antioxidant ability of the fractions. Thin layer chromatography (TLC), high performance liquid chromatography and Fourier transfer infrared spectroscopy (FTIR) were performed to identify and characterize the bioactive component present in each fractions of F. pseudopalma. Results: DPPH and FRAP assay showed that F5, F6 and F7 exhibited the good proton accepting ability and reducing power as compared to the other fractions. All fractions exhibited a good nitric oxide radical scavenging activity wherein F1, F2 and F3 showed the highest inhibition. However, all of the fractions exhibited a stimulatory activity on hydroxyl and superoxide radicals. Lupeol matched one of the spots on the thin layer chromatography chromatogram of the fractions. Linear gradient high performance liquid chromatography and spiking of lupeol with the fraction revealed the presence of 5.84 mg/L lupeol in F6. Infrared spectra of the fractions revealed the presence of C-C, OH, aromatic C=C and C=O groups. Conclusions: The identified lupeol in F. pseudopalma may be responsible for the exhibited antioxidant property of the plant. Furthermore, knowing the antioxidant capability of the plant, F. pseudopalma can be developed into products which can help prevent the occurrence of oxidative stress related diseases. |
format |
Article in Journal/Newspaper |
author |
Librado A Santiago Anna Beatriz R Mayor |
author_facet |
Librado A Santiago Anna Beatriz R Mayor |
author_sort |
Librado A Santiago |
title |
Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
title_short |
Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
title_full |
Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
title_fullStr |
Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
title_full_unstemmed |
Lupeol: An antioxidant triterpene in Ficus pseudopalma Blanco (Moraceae) |
title_sort |
lupeol: an antioxidant triterpene in ficus pseudopalma blanco (moraceae) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.1016/S2221-1691(14)60218-5 https://doaj.org/article/a2ae2c827b144e0988190fe9bc73c477 |
long_lat |
ENVELOPE(-55.233,-55.233,-61.250,-61.250) ENVELOPE(161.917,161.917,-74.333,-74.333) |
geographic |
Arctic Blanco Fenton |
geographic_facet |
Arctic Blanco Fenton |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 2, Pp 109-118 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115301799 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/S2221-1691(14)60218-5 https://doaj.org/article/a2ae2c827b144e0988190fe9bc73c477 |
op_doi |
https://doi.org/10.1016/S2221-1691(14)60218-5 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
2 |
container_start_page |
109 |
op_container_end_page |
118 |
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1766342482573918208 |