Interaction of rheumatoid factor and Entamoeba histolytica

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: P.G. Kremsner, W. Graninger
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1989
• Subjects: Entamoeba histolytica; Amoebae's lysis; Rheumatoid factor; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651989000400001; https://doaj.org/article/a2a2b7b74e68460a92611efc7435ed52

The amoebae's cytotoxicity test and the amoebae's lysis test were used to show possible interactions between rheumatoid factor (RF) and Entamoeba histolytica. Amoebae's cytotoxic activity (ACA) was inhibited by affinity chromatography purified antiamoebae rabbit IgG (RIgG). Enhanced inhibition could be demonstrated with RIgG plus RF. But the same marked inhibition of ACA could be seen when replacing RF by heat inactivated normal human serum as a control. About 50% amoebae's lysis occurred when amoebae were brought together with native normal human serum (NNHS) as a source of complement. Amoebae's lysis increased to 60% when incubated with NHS plus human antiamoebae antibodies. No further augmentation could be obtained by the addition of RF. Using RIgG instead of human antibodies the lysis rate did not increase. Incubation of amoebae, NNHS, RIgG and RF even reduced amoebae's lysis. RF neither has an effect on ACA nor on complement mediated AL in vitro.