Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study
Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakes...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:a286dff0521f4dd4942fdc062f47bba6 2023-05-15T15:14:21+02:00 Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study Ahmed Nafis Ayoub Kasrati Asma Azmani Yedir Ouhdouch Lahcen Hassani 2018-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.244160 https://doaj.org/article/a286dff0521f4dd4942fdc062f47bba6 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=10;spage=513;epage=518;aulast=Nafis https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.244160 https://doaj.org/article/a286dff0521f4dd4942fdc062f47bba6 Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 10, Pp 513-518 (2018) actinobacteria medicinal plant aloe vera antimicrobial activity cytotoxicity antioxidant assays molecular identification Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2018 ftdoajarticles https://doi.org/10.4103/2221-1691.244160 2022-12-31T11:26:14Z Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakesh, Morocco using Bennett agar as selective medium. NAF-1 was tested for its antimicrobial activity against five pathogenic bacteria such as Staphylococcus aureus PIC 53156, Micrococcus luteus ATCC381, Bacillus subtilis ATCC 14579, Pseudomonas aeruginosa DSM 50090 and Escherichia coli ATCC 8739 and four human clinic fungi belonging to the Candida, Aspergillus and Microsporum genera. Several antioxidant activities were studied such as DPPH free radical scavenging, β -carotene and linoleic acid and reducing power assays. The total of phenol and flavonoid was also calculated. Using Artemia salina shrimp assay, the cytotoxicity of NAF-1 crude extract was determined. Results: The results revealed that the actinobacteria showed a high activity (≥20 mm) against only Gram positive bacteria but it had a moderate activity (between 13 and 15 mm) against Human clinic fungi. The isolate also exhibited a LD50 of 14.20 μg/mL in the cytotoxicity assay. The result showed that the crude extract presented an interesting free radical-scavenging activity with IC50 value of (5.58 ± 0.26) μg/mL and a high value of phenolic and flavonoid compounds with (15.41 ± 0.18) μg GAE/mg extract and (11.41± 0.06) μg QE/mg extract respectively. Moreover, the taxonomic position of our endophyte actinobacteria using the morphological and physiological criteria and using 16S rRNA gene sequence (polyphasic approach) showed that the NAF-1 isolate was similar to Streptomyces hydrogenans which was never described as an endophyte actinobacteria. Conclusions: This isolated strain appears promising resources of bioactive agents and can be exploited to produce therapeutic agents active against pathogenic disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 8 10 513 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
actinobacteria medicinal plant aloe vera antimicrobial activity cytotoxicity antioxidant assays molecular identification Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
actinobacteria medicinal plant aloe vera antimicrobial activity cytotoxicity antioxidant assays molecular identification Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Ahmed Nafis Ayoub Kasrati Asma Azmani Yedir Ouhdouch Lahcen Hassani Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
topic_facet |
actinobacteria medicinal plant aloe vera antimicrobial activity cytotoxicity antioxidant assays molecular identification Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To explore the new sources of novel bioactive compounds having pharmaceutical and agricultural interest and to search the endophytic actinobacteria from medicinal plants. Methods: NAF-1 an endophyte actinobacteria was isolated from leaves of medicinal plant Aloe vera collected in Marrakesh, Morocco using Bennett agar as selective medium. NAF-1 was tested for its antimicrobial activity against five pathogenic bacteria such as Staphylococcus aureus PIC 53156, Micrococcus luteus ATCC381, Bacillus subtilis ATCC 14579, Pseudomonas aeruginosa DSM 50090 and Escherichia coli ATCC 8739 and four human clinic fungi belonging to the Candida, Aspergillus and Microsporum genera. Several antioxidant activities were studied such as DPPH free radical scavenging, β -carotene and linoleic acid and reducing power assays. The total of phenol and flavonoid was also calculated. Using Artemia salina shrimp assay, the cytotoxicity of NAF-1 crude extract was determined. Results: The results revealed that the actinobacteria showed a high activity (≥20 mm) against only Gram positive bacteria but it had a moderate activity (between 13 and 15 mm) against Human clinic fungi. The isolate also exhibited a LD50 of 14.20 μg/mL in the cytotoxicity assay. The result showed that the crude extract presented an interesting free radical-scavenging activity with IC50 value of (5.58 ± 0.26) μg/mL and a high value of phenolic and flavonoid compounds with (15.41 ± 0.18) μg GAE/mg extract and (11.41± 0.06) μg QE/mg extract respectively. Moreover, the taxonomic position of our endophyte actinobacteria using the morphological and physiological criteria and using 16S rRNA gene sequence (polyphasic approach) showed that the NAF-1 isolate was similar to Streptomyces hydrogenans which was never described as an endophyte actinobacteria. Conclusions: This isolated strain appears promising resources of bioactive agents and can be exploited to produce therapeutic agents active against pathogenic disease. |
format |
Article in Journal/Newspaper |
author |
Ahmed Nafis Ayoub Kasrati Asma Azmani Yedir Ouhdouch Lahcen Hassani |
author_facet |
Ahmed Nafis Ayoub Kasrati Asma Azmani Yedir Ouhdouch Lahcen Hassani |
author_sort |
Ahmed Nafis |
title |
Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
title_short |
Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
title_full |
Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
title_fullStr |
Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
title_full_unstemmed |
Endophytic actinobacteria of medicinal plant Aloe vera: Isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
title_sort |
endophytic actinobacteria of medicinal plant aloe vera: isolation, antimicrobial, antioxidant, cytotoxicity assays and taxonomic study |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/2221-1691.244160 https://doaj.org/article/a286dff0521f4dd4942fdc062f47bba6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 8, Iss 10, Pp 513-518 (2018) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2018;volume=8;issue=10;spage=513;epage=518;aulast=Nafis https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.244160 https://doaj.org/article/a286dff0521f4dd4942fdc062f47bba6 |
op_doi |
https://doi.org/10.4103/2221-1691.244160 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
8 |
container_issue |
10 |
container_start_page |
513 |
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1766344814968700928 |