Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing
This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering...
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ftdoajarticles:oai:doaj.org/article:a2419ec44b8e44cf8cd07ed79fec3b61 2023-05-15T13:56:43+02:00 Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing Antonio Picazo Carlos Rochera Juan Antonio Villaescusa Javier Miralles-Lorenzo David Velázquez Antonio Quesada Antonio Camacho 2019-04-01T00:00:00Z https://doi.org/10.3389/fmicb.2019.00908 https://doaj.org/article/a2419ec44b8e44cf8cd07ed79fec3b61 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2019.00908/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.00908 https://doaj.org/article/a2419ec44b8e44cf8cd07ed79fec3b61 Frontiers in Microbiology, Vol 10 (2019) next-generation sequencing Byers Peninsula bacterioplankton Maritime Antarctic lakes environmental gradients Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.3389/fmicb.2019.00908 2022-12-31T12:21:07Z This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering the environmental gradient from inland to coastal lakes, some of them sampled both in surface and deep waters. Analysis provided just over 85,000 high quality sequences that were clustered into 864 unique Zero-radius Operational Taxonomic Units (ZOTUs) (i.e., 100% sequence similarity). Yet, several taxonomic uncertainties remained in the analysis likely suggesting the occurrence of local bacterial adaptations. The survey showed the dominance of the phyla Proteobacteria and Bacteroidetes. Among the former, the Gammaproteobacteria class, more specifically the order Betaproteobacteriales, was the dominant group, which seems to be a common trend in nutrient-limited Antarctic lakes. Most of the families and genera ubiquitously detected belonging to this class are indeed typical from ultra-oligotrophic environments, and commonly described as diazotrophs. On the other hand, among the members of the phylum Bacteroidetes, genera such as Flavobacterium were abundant in some of the shallowest lakes, thus demonstrating that also benthic and sediment-associated bacteria contributed to water bacterial assemblages. Ordination analyses sorted bacterial assemblages mainly based on the environmental gradients of nutrient availability and conductivity i.e., salinity. However, transient bacterial associations, that included the groups Clostridiaceae and Chloroflexi, also occurred as being forced by other drivers such as the influence of the nearby fauna and by the airborne microorganisms. As we intended, our NGS-based approach has provided a much greater resolution compared to the previous studies conducted in the area and confirmed to a large extent the previously obtained patterns, thus reinforcing the view of Byers as a hotspot of ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic Byers ENVELOPE(-60.283,-60.283,-63.900,-63.900) Byers peninsula ENVELOPE(-61.066,-61.066,-62.633,-62.633) Frontiers in Microbiology 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
next-generation sequencing Byers Peninsula bacterioplankton Maritime Antarctic lakes environmental gradients Microbiology QR1-502 |
spellingShingle |
next-generation sequencing Byers Peninsula bacterioplankton Maritime Antarctic lakes environmental gradients Microbiology QR1-502 Antonio Picazo Carlos Rochera Juan Antonio Villaescusa Javier Miralles-Lorenzo David Velázquez Antonio Quesada Antonio Camacho Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
topic_facet |
next-generation sequencing Byers Peninsula bacterioplankton Maritime Antarctic lakes environmental gradients Microbiology QR1-502 |
description |
This study comprises the first attempt to describe the planktonic bacterial communities of lakes from Byers Peninsula, one of the most significant limnological districts in the Maritime Antarctica, leveraging next-generation sequencing (NGS) technologies. For the survey, we selected 7 lakes covering the environmental gradient from inland to coastal lakes, some of them sampled both in surface and deep waters. Analysis provided just over 85,000 high quality sequences that were clustered into 864 unique Zero-radius Operational Taxonomic Units (ZOTUs) (i.e., 100% sequence similarity). Yet, several taxonomic uncertainties remained in the analysis likely suggesting the occurrence of local bacterial adaptations. The survey showed the dominance of the phyla Proteobacteria and Bacteroidetes. Among the former, the Gammaproteobacteria class, more specifically the order Betaproteobacteriales, was the dominant group, which seems to be a common trend in nutrient-limited Antarctic lakes. Most of the families and genera ubiquitously detected belonging to this class are indeed typical from ultra-oligotrophic environments, and commonly described as diazotrophs. On the other hand, among the members of the phylum Bacteroidetes, genera such as Flavobacterium were abundant in some of the shallowest lakes, thus demonstrating that also benthic and sediment-associated bacteria contributed to water bacterial assemblages. Ordination analyses sorted bacterial assemblages mainly based on the environmental gradients of nutrient availability and conductivity i.e., salinity. However, transient bacterial associations, that included the groups Clostridiaceae and Chloroflexi, also occurred as being forced by other drivers such as the influence of the nearby fauna and by the airborne microorganisms. As we intended, our NGS-based approach has provided a much greater resolution compared to the previous studies conducted in the area and confirmed to a large extent the previously obtained patterns, thus reinforcing the view of Byers as a hotspot of ... |
format |
Article in Journal/Newspaper |
author |
Antonio Picazo Carlos Rochera Juan Antonio Villaescusa Javier Miralles-Lorenzo David Velázquez Antonio Quesada Antonio Camacho |
author_facet |
Antonio Picazo Carlos Rochera Juan Antonio Villaescusa Javier Miralles-Lorenzo David Velázquez Antonio Quesada Antonio Camacho |
author_sort |
Antonio Picazo |
title |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_short |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_full |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_fullStr |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_full_unstemmed |
Bacterioplankton Community Composition Along Environmental Gradients in Lakes From Byers Peninsula (Maritime Antarctica) as Determined by Next-Generation Sequencing |
title_sort |
bacterioplankton community composition along environmental gradients in lakes from byers peninsula (maritime antarctica) as determined by next-generation sequencing |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.00908 https://doaj.org/article/a2419ec44b8e44cf8cd07ed79fec3b61 |
long_lat |
ENVELOPE(-60.283,-60.283,-63.900,-63.900) ENVELOPE(-61.066,-61.066,-62.633,-62.633) |
geographic |
Antarctic Byers Byers peninsula |
geographic_facet |
Antarctic Byers Byers peninsula |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Microbiology, Vol 10 (2019) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2019.00908/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2019.00908 https://doaj.org/article/a2419ec44b8e44cf8cd07ed79fec3b61 |
op_doi |
https://doi.org/10.3389/fmicb.2019.00908 |
container_title |
Frontiers in Microbiology |
container_volume |
10 |
_version_ |
1766264291681370112 |