Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to ada...
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ftdoajarticles:oai:doaj.org/article:a202ef87b54547dab5a40c3f61968df8 2023-05-15T17:50:19+02:00 Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress J. Wang B. D. Russell M.-W. Ding Y.-W. Dong 2018-05-01T00:00:00Z https://doi.org/10.5194/bg-15-2803-2018 https://doaj.org/article/a202ef87b54547dab5a40c3f61968df8 EN eng Copernicus Publications https://www.biogeosciences.net/15/2803/2018/bg-15-2803-2018.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-15-2803-2018 1726-4170 1726-4189 https://doaj.org/article/a202ef87b54547dab5a40c3f61968df8 Biogeosciences, Vol 15, Pp 2803-2817 (2018) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2018 ftdoajarticles https://doi.org/10.5194/bg-15-2803-2018 2022-12-31T09:40:45Z Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different p CO 2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day p CO 2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins ( hsp70 and hsc70 ) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients ( Q 10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm p CO 2 ). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response ... Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 15 9 2803 2817 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 J. Wang B. D. Russell M.-W. Ding Y.-W. Dong Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Understanding physiological responses of organisms to warming and ocean acidification is the first step towards predicting the potential population- and community-level ecological impacts of these stressors. Increasingly, physiological plasticity is being recognized as important for organisms to adapt to the changing microclimates. Here, we evaluate the importance of physiological plasticity for coping with ocean acidification and elevated temperature, and its variability among individuals, of the intertidal limpet Cellana toreuma from the same population in Xiamen. Limpets were collected from shaded mid-intertidal rock surfaces. They were acclimated under combinations of different p CO 2 concentrations (400 and 1000 ppm, corresponding to a pH of 8.1 and 7.8) and temperatures (20 and 24 °C) in a short-term period (7 days), with the control conditions (20 °C and 400 ppm) representing the average annual temperature and present-day p CO 2 level at the collection site. Heart rates (as a proxy for metabolic performance) and expression of genes encoding inducible and constitutive heat-shock proteins ( hsp70 and hsc70 ) at different heat-shock temperatures (26, 30, 34, and 38 °C) were measured. Hsp70 and Hsc70 play important roles in protecting cells from heat stresses, but have different expression patterns, with Hsp70 significantly increased in expression during stress and Hsc70 constitutively expressed and only mildly induced during stress. Analysis of heart rate showed significantly higher temperature coefficients ( Q 10 rates) for limpets at 20 °C than at 24 °C and post-acclimation thermal sensitivity of limpets at 400 ppm was lower than at 1000 ppm. Expression of hsp70 linearly increased with the increasing heat-shock temperatures, with the largest slope occurring in limpets acclimated under a future scenario (24 °C and 1000 ppm p CO 2 ). These results suggested that limpets showed increased sensitivity and stress response under future conditions. Furthermore, the increased variation in physiological response ... |
format |
Article in Journal/Newspaper |
author |
J. Wang B. D. Russell M.-W. Ding Y.-W. Dong |
author_facet |
J. Wang B. D. Russell M.-W. Ding Y.-W. Dong |
author_sort |
J. Wang |
title |
Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_short |
Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_full |
Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_fullStr |
Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_full_unstemmed |
Ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
title_sort |
ocean acidification increases the sensitivity of and variability in physiological responses of an intertidal limpet to thermal stress |
publisher |
Copernicus Publications |
publishDate |
2018 |
url |
https://doi.org/10.5194/bg-15-2803-2018 https://doaj.org/article/a202ef87b54547dab5a40c3f61968df8 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 15, Pp 2803-2817 (2018) |
op_relation |
https://www.biogeosciences.net/15/2803/2018/bg-15-2803-2018.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-15-2803-2018 1726-4170 1726-4189 https://doaj.org/article/a202ef87b54547dab5a40c3f61968df8 |
op_doi |
https://doi.org/10.5194/bg-15-2803-2018 |
container_title |
Biogeosciences |
container_volume |
15 |
container_issue |
9 |
container_start_page |
2803 |
op_container_end_page |
2817 |
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1766157027308994560 |