Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species
Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RES...
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ftdoajarticles:oai:doaj.org/article:a1e3dbd735d5465282a6b4b59aac306f 2023-05-15T15:01:29+02:00 Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species Lucas Eduardo Trevisoli Larissa Bail Luiza Souza Rodrigues Danieli Conte Jussara Kasuko Palmeiro Libera Maria Dalla-Costa https://doi.org/10.1590/0037-8682-0026-2017 https://doaj.org/article/a1e3dbd735d5465282a6b4b59aac306f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000100085&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0026-2017 https://doaj.org/article/a1e3dbd735d5465282a6b4b59aac306f Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 1, Pp 85-87 Coagulase-negative staphylococci MALDI-TOF tuf gene Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0026-2017 2022-12-31T02:57:30Z Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RESULTS. The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate). CONCLUSIONS . MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%). Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 51 1 85 87 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Coagulase-negative staphylococci MALDI-TOF tuf gene Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Coagulase-negative staphylococci MALDI-TOF tuf gene Arctic medicine. Tropical medicine RC955-962 Lucas Eduardo Trevisoli Larissa Bail Luiza Souza Rodrigues Danieli Conte Jussara Kasuko Palmeiro Libera Maria Dalla-Costa Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
topic_facet |
Coagulase-negative staphylococci MALDI-TOF tuf gene Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION. This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS. A total of 134 CoNS strains were characterized using four different methods. RESULTS. The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1.00). The kappa index of Vitek 2® Compact analysis was 0.85 (very good) and that of the conventional method was 0.63 (moderate). CONCLUSIONS . MALDI-TOF MS provided rapid and accurate results for the identification of CoNS (134; 100%). |
format |
Article in Journal/Newspaper |
author |
Lucas Eduardo Trevisoli Larissa Bail Luiza Souza Rodrigues Danieli Conte Jussara Kasuko Palmeiro Libera Maria Dalla-Costa |
author_facet |
Lucas Eduardo Trevisoli Larissa Bail Luiza Souza Rodrigues Danieli Conte Jussara Kasuko Palmeiro Libera Maria Dalla-Costa |
author_sort |
Lucas Eduardo Trevisoli |
title |
Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
title_short |
Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
title_full |
Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
title_fullStr |
Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
title_full_unstemmed |
Matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative Staphylococci species |
title_sort |
matrix-assisted laser desorption ionization-time of flight: a promising alternative method of identifying the major coagulase-negative staphylococci species |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0026-2017 https://doaj.org/article/a1e3dbd735d5465282a6b4b59aac306f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 1, Pp 85-87 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000100085&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0026-2017 https://doaj.org/article/a1e3dbd735d5465282a6b4b59aac306f |
op_doi |
https://doi.org/10.1590/0037-8682-0026-2017 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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51 |
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