Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species
Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mito...
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ftdoajarticles:oai:doaj.org/article:a1de3df480794946b5c7a71dc5ae4047 2023-08-27T04:05:38+02:00 Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti 2022-07-01T00:00:00Z https://doi.org/10.3390/biology11081118 https://doaj.org/article/a1de3df480794946b5c7a71dc5ae4047 EN eng MDPI AG https://www.mdpi.com/2079-7737/11/8/1118 https://doaj.org/toc/2079-7737 doi:10.3390/biology11081118 2079-7737 https://doaj.org/article/a1de3df480794946b5c7a71dc5ae4047 Biology, Vol 11, Iss 1118, p 1118 (2022) icefish proteomics mitochondria muscle network analysis notothenioid Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/biology11081118 2023-08-06T00:43:48Z Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mitochondrial proteomes of both the red and white muscle of two different icefish species ( Champsocephalus gunnari and Chionodraco rastrospinosus) and compared these with two related red-blooded Notothenioids ( Notothenia rossii , Trematomus bernacchii ). Liquid Chromatography-Mass spectrometry (LC-MS/MS) was used to generate and examine the proteomic profiles of the two groups. We recorded a total of 91 differentially expressed proteins in the icefish red muscle mitochondria and 89 in the white muscle mitochondria when compared with the red-blooded related species. The icefish have a relatively higher abundance of proteins involved with Complex V of oxidative phosphorylation, RNA metabolism, and homeostasis, and fewer proteins for striated muscle contraction, haem, iron, creatine, and carbohydrate metabolism. Enrichment analyses showed that many important pathways were different in both red muscle and white muscle, including the citric acid cycle, ribosome machinery and fatty acid degradation. Life in the Antarctic waters poses extra challenges to the organisms that reside within them. Icefish have successfully inhabited this environment and we surmise that species without haemoglobin uniquely maintain their physiology. Our study highlights the mitochondrial protein pathway differences between similar fish species according to their specific tissue oxygenation idiosyncrasies. Article in Journal/Newspaper Antarc* Antarctic Icefish Notothenia rossii Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Biology 11 8 1118 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
icefish proteomics mitochondria muscle network analysis notothenioid Biology (General) QH301-705.5 |
spellingShingle |
icefish proteomics mitochondria muscle network analysis notothenioid Biology (General) QH301-705.5 Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
topic_facet |
icefish proteomics mitochondria muscle network analysis notothenioid Biology (General) QH301-705.5 |
description |
Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mitochondrial proteomes of both the red and white muscle of two different icefish species ( Champsocephalus gunnari and Chionodraco rastrospinosus) and compared these with two related red-blooded Notothenioids ( Notothenia rossii , Trematomus bernacchii ). Liquid Chromatography-Mass spectrometry (LC-MS/MS) was used to generate and examine the proteomic profiles of the two groups. We recorded a total of 91 differentially expressed proteins in the icefish red muscle mitochondria and 89 in the white muscle mitochondria when compared with the red-blooded related species. The icefish have a relatively higher abundance of proteins involved with Complex V of oxidative phosphorylation, RNA metabolism, and homeostasis, and fewer proteins for striated muscle contraction, haem, iron, creatine, and carbohydrate metabolism. Enrichment analyses showed that many important pathways were different in both red muscle and white muscle, including the citric acid cycle, ribosome machinery and fatty acid degradation. Life in the Antarctic waters poses extra challenges to the organisms that reside within them. Icefish have successfully inhabited this environment and we surmise that species without haemoglobin uniquely maintain their physiology. Our study highlights the mitochondrial protein pathway differences between similar fish species according to their specific tissue oxygenation idiosyncrasies. |
format |
Article in Journal/Newspaper |
author |
Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti |
author_facet |
Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti |
author_sort |
Gunjan Katyal |
title |
Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
title_short |
Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
title_full |
Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
title_fullStr |
Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
title_full_unstemmed |
Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
title_sort |
quantitative proteomics and network analysis of differentially expressed proteins in proteomes of icefish muscle mitochondria compared with closely related red-blooded species |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11081118 https://doaj.org/article/a1de3df480794946b5c7a71dc5ae4047 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Icefish Notothenia rossii |
genre_facet |
Antarc* Antarctic Icefish Notothenia rossii |
op_source |
Biology, Vol 11, Iss 1118, p 1118 (2022) |
op_relation |
https://www.mdpi.com/2079-7737/11/8/1118 https://doaj.org/toc/2079-7737 doi:10.3390/biology11081118 2079-7737 https://doaj.org/article/a1de3df480794946b5c7a71dc5ae4047 |
op_doi |
https://doi.org/10.3390/biology11081118 |
container_title |
Biology |
container_volume |
11 |
container_issue |
8 |
container_start_page |
1118 |
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1775357363324190720 |