Eutypellenoids A–C, New Pimarane Diterpenes from the Arctic Fungus Eutypella sp. D-1

• Published in: Marine Drugs
• Main Authors: Hao-Bing Yu, Xiao-Li Wang, Wei-Heng Xu, Yi-Xin Zhang, Yi-Sen Qian, Jian-Peng Zhang, Xiao-Ling Lu, Xiao-Yu Liu
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2018
• Subjects: Arctic fungus; Eutypella sp; pimarane diterpene; bioactivity; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.3390/md16080284; https://doaj.org/article/a1cf1f561b2f4d389b2337b6f8998f8d

Three new pimarane diterpenes, eutypellenoids A–C (1–3), together with a known compound, eutypenoid C (4), were isolated from the culture extract of Eutypella sp. D-1 derived from the Arctic region. Compounds 1–3 possessed an uncommon tetrahydrofuran-fused pimarane diterpene skeleton. The structures of all compounds were determined by detailed spectroscopic analysis, electronic circular dichroism (ECD) analysis, as well as a comparison with the literature data. Antibacterial, antifungal, and cytotoxic activities of these compounds were evaluated. Compound 2 displayed antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 8 and 8 μg/mL, respectively. Additionally, compound 2 showed antifungal activity against Candida parapsilosis, Candida albicans, Candida glabrata, and Candida tropicalis with MIC values of 8, 8, 16, and 32 μg/mL, respectively. Furthermore, compound 2 exhibited moderate cytotoxic activity against HCT-116 cell line with IC50 value of 3.7 μM.