Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming
Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiseru...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992005000300010 https://doaj.org/article/a1b35c0400d04090a308a45b00e8f76d |
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ftdoajarticles:oai:doaj.org/article:a1b35c0400d04090a308a45b00e8f76d 2023-05-15T15:04:04+02:00 Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming J. Ferreira M. F. Bastos A. C. Pelizon C. M. Peres D. P. Cavalcante A. Sartori 2005-09-01T00:00:00Z https://doi.org/10.1590/S1678-91992005000300010 https://doaj.org/article/a1b35c0400d04090a308a45b00e8f76d EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300010 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000300010 1678-9199 https://doaj.org/article/a1b35c0400d04090a308a45b00e8f76d Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 3, Pp 336-349 (2005) Bothrops jararaca venom ovine antiserum venom levels edema ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2005 ftdoajarticles https://doi.org/10.1590/S1678-91992005000300010 2022-12-31T00:58:27Z Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 11 3 336 349 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bothrops jararaca venom ovine antiserum venom levels edema ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Bothrops jararaca venom ovine antiserum venom levels edema ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 J. Ferreira M. F. Bastos A. C. Pelizon C. M. Peres D. P. Cavalcante A. Sartori Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
topic_facet |
Bothrops jararaca venom ovine antiserum venom levels edema ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD50) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom. |
format |
Article in Journal/Newspaper |
author |
J. Ferreira M. F. Bastos A. C. Pelizon C. M. Peres D. P. Cavalcante A. Sartori |
author_facet |
J. Ferreira M. F. Bastos A. C. Pelizon C. M. Peres D. P. Cavalcante A. Sartori |
author_sort |
J. Ferreira |
title |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_short |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_full |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_fullStr |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_full_unstemmed |
Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming |
title_sort |
assessment of the neutralizing potency of ovine antivenom in a swiss mice model of bothrops jararaca envenoming |
publisher |
SciELO |
publishDate |
2005 |
url |
https://doi.org/10.1590/S1678-91992005000300010 https://doaj.org/article/a1b35c0400d04090a308a45b00e8f76d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 11, Iss 3, Pp 336-349 (2005) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300010 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992005000300010 1678-9199 https://doaj.org/article/a1b35c0400d04090a308a45b00e8f76d |
op_doi |
https://doi.org/10.1590/S1678-91992005000300010 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
11 |
container_issue |
3 |
container_start_page |
336 |
op_container_end_page |
349 |
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1766335890211209216 |