Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway
Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at mRNA and protein levels in HepG2 cell exposed to Proliverenol for 1 and 3 h. Results: Prolivere...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:a1a4db013d864cf3b8250c84d04e2e8b 2023-05-15T15:09:07+02:00 Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway Guntur Berlian Olivia Mayasari Tandrasasmita Raymond Rubianto Tjandrawinata 2016-08-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.06.007 https://doaj.org/article/a1a4db013d864cf3b8250c84d04e2e8b EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115310388 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.007 https://doaj.org/article/a1a4db013d864cf3b8250c84d04e2e8b Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 686-691 (2016) Phaleria macrocarpa Hepatoprotector Liver injury Cirrhosis HepG2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.06.007 2022-12-31T13:39:34Z Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at mRNA and protein levels in HepG2 cell exposed to Proliverenol for 1 and 3 h. Results: Proliverenol conferred hepatoprotective activity through increasing cell survival up to 53%–69% via up-regulation of APEX1 DNA repair enzyme for 3.0–4.7 fold and down-regulating of nuclear factor-κB, tumor necrosis factorα and caspase-8 expression, allowing them to prevent 4.5–6.9 fold of alanine aminotransferase (ALT) leakage in HepG2 cells. Our finding revealed that Proliverenol repressed expression of ALT, which is significantly important as possible alternative mechanism for increased blood transaminase activities. In addition, the result also showed that caspase-8 pathway seemed to be involved in the molecular pathway rather than directly inducing mitochondrial damage. Conclusions: The data support our hypothesis that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. We propose that Proliverenol provides hepatoprotective activity through up-regulating expression of APEX1 that repress DNA fragmentation, and down-regulating expression of nuclear factor-κB, tumor necrosis factorα and caspase-8, which therefore repress ALT leakage and its expression. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 8 686 691 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Phaleria macrocarpa Hepatoprotector Liver injury Cirrhosis HepG2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Phaleria macrocarpa Hepatoprotector Liver injury Cirrhosis HepG2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Guntur Berlian Olivia Mayasari Tandrasasmita Raymond Rubianto Tjandrawinata Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
topic_facet |
Phaleria macrocarpa Hepatoprotector Liver injury Cirrhosis HepG2 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To verify that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. Methods: Activity of Proliverenol against ethanol-induced apoptosis was evaluated at mRNA and protein levels in HepG2 cell exposed to Proliverenol for 1 and 3 h. Results: Proliverenol conferred hepatoprotective activity through increasing cell survival up to 53%–69% via up-regulation of APEX1 DNA repair enzyme for 3.0–4.7 fold and down-regulating of nuclear factor-κB, tumor necrosis factorα and caspase-8 expression, allowing them to prevent 4.5–6.9 fold of alanine aminotransferase (ALT) leakage in HepG2 cells. Our finding revealed that Proliverenol repressed expression of ALT, which is significantly important as possible alternative mechanism for increased blood transaminase activities. In addition, the result also showed that caspase-8 pathway seemed to be involved in the molecular pathway rather than directly inducing mitochondrial damage. Conclusions: The data support our hypothesis that Proliverenol has a potential ability in protecting cells from ethanol-induced hepatotoxicity. We propose that Proliverenol provides hepatoprotective activity through up-regulating expression of APEX1 that repress DNA fragmentation, and down-regulating expression of nuclear factor-κB, tumor necrosis factorα and caspase-8, which therefore repress ALT leakage and its expression. |
format |
Article in Journal/Newspaper |
author |
Guntur Berlian Olivia Mayasari Tandrasasmita Raymond Rubianto Tjandrawinata |
author_facet |
Guntur Berlian Olivia Mayasari Tandrasasmita Raymond Rubianto Tjandrawinata |
author_sort |
Guntur Berlian |
title |
Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
title_short |
Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
title_full |
Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
title_fullStr |
Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
title_full_unstemmed |
Standardized bioactive fraction of Phaleria macrocarpa (Proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of NF-κB-TNFα-caspase-8 pathway |
title_sort |
standardized bioactive fraction of phaleria macrocarpa (proliverenol) prevents ethanol-induced hepatotoxicity via down-regulation of nf-κb-tnfα-caspase-8 pathway |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.06.007 https://doaj.org/article/a1a4db013d864cf3b8250c84d04e2e8b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 8, Pp 686-691 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115310388 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.06.007 https://doaj.org/article/a1a4db013d864cf3b8250c84d04e2e8b |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.06.007 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
8 |
container_start_page |
686 |
op_container_end_page |
691 |
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