Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia
ABSTRACT Candida is one of the most frequent pathogens of bloodstream infections, which is associated with high morbidity and mortality rates. Rapid immunological detection methods are essential in the early diagnosis of candidemia. Anti-mannan is one of host-derived biomarkers against cell wall com...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
|
Subjects: | |
Online Access: | https://doi.org/10.1590/s1678-9946202062025 https://doaj.org/article/a1a267b7eeab4965b211a395cbece247 |
id |
ftdoajarticles:oai:doaj.org/article:a1a267b7eeab4965b211a395cbece247 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:a1a267b7eeab4965b211a395cbece247 2024-09-09T19:26:47+00:00 Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia Yanming Meng Mei Kang Dongdong Li Tingting Wang Ziwei Kuang Ying Ma https://doi.org/10.1590/s1678-9946202062025 https://doaj.org/article/a1a267b7eeab4965b211a395cbece247 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100214&lng=en&tlng=en https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 doi:10.1590/s1678-9946202062025 https://doaj.org/article/a1a267b7eeab4965b211a395cbece247 Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 candidemia anti-mannan diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article ftdoajarticles https://doi.org/10.1590/s1678-9946202062025 2024-08-05T17:49:31Z ABSTRACT Candida is one of the most frequent pathogens of bloodstream infections, which is associated with high morbidity and mortality rates. Rapid immunological detection methods are essential in the early diagnosis of candidemia. Anti-mannan is one of host-derived biomarkers against cell wall components of Candida. We conducted this study to evaluate the diagnostic performance of two anti-mannan assays (IgM, IgG) for candidemia through the analysis of 40 candidemia patients, 48 participants with Candida colonization and 213 participants with neither Candida colonization nor Candida infections (13 patients with other bloodstream infections, 145 hospitalized patients and 55 healthy controls). The performance of the two assays were evaluated by calculating their sensitivity and specificity. The sensitivity ranged from 0.78 to 0.80 for the IgM assay and 0.68 to 0.75 for the IgG assay. The specificity ranged from 0.97 to 0.98 for the IgM assay and 0.91 to 0.94 for the IgG assay. The diagnostic performance of the anti-mannan IgM assay was better than that of IgG, with higher sensitivity and specificity. Combining the two assays (positive results of single or both assays are both considered as positive) could improve the sensitivity up to 0.93 (0.79-0.98) and only slightly reduce the specificity (0.93(0.89-0.95)). The anti-mannan IgM, IgG assays are rapid and cost-effective assays that may be probably useful in the diagnosis of candidemia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
candidemia anti-mannan diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
candidemia anti-mannan diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Yanming Meng Mei Kang Dongdong Li Tingting Wang Ziwei Kuang Ying Ma Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
topic_facet |
candidemia anti-mannan diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT Candida is one of the most frequent pathogens of bloodstream infections, which is associated with high morbidity and mortality rates. Rapid immunological detection methods are essential in the early diagnosis of candidemia. Anti-mannan is one of host-derived biomarkers against cell wall components of Candida. We conducted this study to evaluate the diagnostic performance of two anti-mannan assays (IgM, IgG) for candidemia through the analysis of 40 candidemia patients, 48 participants with Candida colonization and 213 participants with neither Candida colonization nor Candida infections (13 patients with other bloodstream infections, 145 hospitalized patients and 55 healthy controls). The performance of the two assays were evaluated by calculating their sensitivity and specificity. The sensitivity ranged from 0.78 to 0.80 for the IgM assay and 0.68 to 0.75 for the IgG assay. The specificity ranged from 0.97 to 0.98 for the IgM assay and 0.91 to 0.94 for the IgG assay. The diagnostic performance of the anti-mannan IgM assay was better than that of IgG, with higher sensitivity and specificity. Combining the two assays (positive results of single or both assays are both considered as positive) could improve the sensitivity up to 0.93 (0.79-0.98) and only slightly reduce the specificity (0.93(0.89-0.95)). The anti-mannan IgM, IgG assays are rapid and cost-effective assays that may be probably useful in the diagnosis of candidemia. |
format |
Article in Journal/Newspaper |
author |
Yanming Meng Mei Kang Dongdong Li Tingting Wang Ziwei Kuang Ying Ma |
author_facet |
Yanming Meng Mei Kang Dongdong Li Tingting Wang Ziwei Kuang Ying Ma |
author_sort |
Yanming Meng |
title |
Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
title_short |
Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
title_full |
Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
title_fullStr |
Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
title_full_unstemmed |
Performance of a new Candida anti-mannan IgM and IgG assays in the diagnosis of candidemia |
title_sort |
performance of a new candida anti-mannan igm and igg assays in the diagnosis of candidemia |
publisher |
Universidade de São Paulo (USP) |
url |
https://doi.org/10.1590/s1678-9946202062025 https://doaj.org/article/a1a267b7eeab4965b211a395cbece247 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 62 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652020000100214&lng=en&tlng=en https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 doi:10.1590/s1678-9946202062025 https://doaj.org/article/a1a267b7eeab4965b211a395cbece247 |
op_doi |
https://doi.org/10.1590/s1678-9946202062025 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
62 |
_version_ |
1809896336911237120 |