Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest
Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier, Mt. Everest was incubation in two incubation ways: plate melt water directly and enrichment melt water prior plate, respectively. The abundance of cultivable bacteria ranged from 0–295 CFU mL−1 to 0–1720 CFU mL−1...
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ftdoajarticles:oai:doaj.org/article:a1663f96a81341caaaf9cd35c86dbe07 2023-10-01T03:56:37+02:00 Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu 2012-05-01T00:00:00Z https://doi.org/10.1016/j.gsf.2011.12.013 https://doaj.org/article/a1663f96a81341caaaf9cd35c86dbe07 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S1674987112000023 https://doaj.org/toc/1674-9871 1674-9871 doi:10.1016/j.gsf.2011.12.013 https://doaj.org/article/a1663f96a81341caaaf9cd35c86dbe07 Geoscience Frontiers, Vol 3, Iss 3, Pp 327-334 (2012) Tibetan Plateau Ice core Cultivable bacteria Geology QE1-996.5 article 2012 ftdoajarticles https://doi.org/10.1016/j.gsf.2011.12.013 2023-09-03T00:34:56Z Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier, Mt. Everest was incubation in two incubation ways: plate melt water directly and enrichment melt water prior plate, respectively. The abundance of cultivable bacteria ranged from 0–295 CFU mL−1 to 0–1720 CFU mL−1 in two incubations with a total of 1385 isolates obtained. Comparing to direct cultivation, enrichment cultivation recovered more bacteria. Pigment-producing bacteria accounted for an average of 84.9% of total isolates. Such high percentage suggested that pigment production may be an adaptive physiological feature for the bacteria in ice core to cope with strong ultraviolet radiation on the glacier. The abundances of cultivable bacteria and pigment-producing isolates varied synchronously along depth: higher abundance in the middle and lower at the top and bottom. It indicated that the middle part of the ice core was hospitable for the microbial survival. Based on the physiological properties of the colonies, eighty-nine isolates were selected for phylogenetic analysis. Obtained 16S rRNA gene sequences fell into four groups: Firmicutes, Alpha-Proteobacteria, Gamma-Proteobacteria, and Actinobacteria, with the Firmicutes being dominant. Microbial compositions derived from direct and enrichment cultivations were not overlapped. We suggest that it is a better way to explore the culturable microbial diversity in ice core by combining the approaches of both direct and enrichment cultivation. Article in Journal/Newspaper ice core Directory of Open Access Journals: DOAJ Articles Geoscience Frontiers 3 3 327 334 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tibetan Plateau Ice core Cultivable bacteria Geology QE1-996.5 |
spellingShingle |
Tibetan Plateau Ice core Cultivable bacteria Geology QE1-996.5 Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
topic_facet |
Tibetan Plateau Ice core Cultivable bacteria Geology QE1-996.5 |
description |
Ice melt water from a 22.27 m ice core which was drilled from the East Rongbuk Glacier, Mt. Everest was incubation in two incubation ways: plate melt water directly and enrichment melt water prior plate, respectively. The abundance of cultivable bacteria ranged from 0–295 CFU mL−1 to 0–1720 CFU mL−1 in two incubations with a total of 1385 isolates obtained. Comparing to direct cultivation, enrichment cultivation recovered more bacteria. Pigment-producing bacteria accounted for an average of 84.9% of total isolates. Such high percentage suggested that pigment production may be an adaptive physiological feature for the bacteria in ice core to cope with strong ultraviolet radiation on the glacier. The abundances of cultivable bacteria and pigment-producing isolates varied synchronously along depth: higher abundance in the middle and lower at the top and bottom. It indicated that the middle part of the ice core was hospitable for the microbial survival. Based on the physiological properties of the colonies, eighty-nine isolates were selected for phylogenetic analysis. Obtained 16S rRNA gene sequences fell into four groups: Firmicutes, Alpha-Proteobacteria, Gamma-Proteobacteria, and Actinobacteria, with the Firmicutes being dominant. Microbial compositions derived from direct and enrichment cultivations were not overlapped. We suggest that it is a better way to explore the culturable microbial diversity in ice core by combining the approaches of both direct and enrichment cultivation. |
format |
Article in Journal/Newspaper |
author |
Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu |
author_facet |
Liang Shen Tandong Yao Baiqing Xu Hongmei Wang Nianzhi Jiao Shichang Kang Xiaobo Liu Yongqin Liu |
author_sort |
Liang Shen |
title |
Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
title_short |
Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
title_full |
Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
title_fullStr |
Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
title_full_unstemmed |
Variation of culturable bacteria along depth in the East Rongbuk ice core, Mt. Everest |
title_sort |
variation of culturable bacteria along depth in the east rongbuk ice core, mt. everest |
publisher |
Elsevier |
publishDate |
2012 |
url |
https://doi.org/10.1016/j.gsf.2011.12.013 https://doaj.org/article/a1663f96a81341caaaf9cd35c86dbe07 |
genre |
ice core |
genre_facet |
ice core |
op_source |
Geoscience Frontiers, Vol 3, Iss 3, Pp 327-334 (2012) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S1674987112000023 https://doaj.org/toc/1674-9871 1674-9871 doi:10.1016/j.gsf.2011.12.013 https://doaj.org/article/a1663f96a81341caaaf9cd35c86dbe07 |
op_doi |
https://doi.org/10.1016/j.gsf.2011.12.013 |
container_title |
Geoscience Frontiers |
container_volume |
3 |
container_issue |
3 |
container_start_page |
327 |
op_container_end_page |
334 |
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1778526611906232320 |