Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia.
Information on the insecticide resistance profiles of Aedes aegypti in Indonesia is fragmentary because of the lack of wide-area insecticide resistance surveillance. We collected Ae. aegypti from 32 districts and regencies in 27 Indonesian provinces and used WHO bioassays to evaluate their resistanc...
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ftdoajarticles:oai:doaj.org/article:a14e58984e834d28b30eefb2e4b4ccd1 2023-05-15T15:12:12+02:00 Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. Christina Natalina Silalahi Wu-Chun Tu Niann-Tai Chang G Veera Singham Intan Ahmad Kok-Boon Neoh 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010501 https://doaj.org/article/a14e58984e834d28b30eefb2e4b4ccd1 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010501 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010501 https://doaj.org/article/a14e58984e834d28b30eefb2e4b4ccd1 PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010501 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010501 2022-12-30T23:19:49Z Information on the insecticide resistance profiles of Aedes aegypti in Indonesia is fragmentary because of the lack of wide-area insecticide resistance surveillance. We collected Ae. aegypti from 32 districts and regencies in 27 Indonesian provinces and used WHO bioassays to evaluate their resistance to deltamethrin, permethrin, bendiocarb, and pirimiphos-methyl. To determine the possible resistance mechanisms of Ae. aegypti, synergism tests were conducted using piperonyl butoxide (PBO) and S,S,S-tributylphosphorotrithioates (DEF). The Ae. aegypti from all locations exhibited various levels of resistance to pyrethroids. Their resistance ratio (RR50) to permethrin and deltamethrin ranged from 4.08× to 127× and from 4.37× to 72.20×, respectively. In contrast with the findings of other studies, most strains from the highly urbanized cities on the island of Java (i.e., Banten, Jakarta, Bandung, Semarang, Yogyakarta, and Surabaya) exhibited low to moderate resistance to pyrethroids. By contrast, the strains collected from the less populated Kalimantan region exhibited very high resistance to pyrethroids. The possible reasons are discussed herein. Low levels of resistance to bendiocarb (RR50, 1.24-6.46×) and pirimiphos-methyl (RR50, 1.01-2.70×) were observed in all tested strains, regardless of locality. PBO and DEF synergists significantly increased the susceptibility of Ae. aegypti to permethrin and deltamethrin and reduced their resistance ratio to less than 16×. The synergism tests suggested the major involvement of cytochrome P450 monooxygenases and esterases in conferring pyrethroid resistance. On the basis of our results, we proposed a 6-month rotation of insecticides (deltamethrin + synergists ➝ bendiocarb ➝ permethrin + synergists ➝ pirimiphos-methyl) and the use of an insecticide mixture containing pyrethroid and pyrimiphos-methyl to control Ae. aegypti populations and overcome the challenge of widespread Ae. aegypti resistance to pyrethroid in Indonesia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 6 e0010501 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Christina Natalina Silalahi Wu-Chun Tu Niann-Tai Chang G Veera Singham Intan Ahmad Kok-Boon Neoh Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Information on the insecticide resistance profiles of Aedes aegypti in Indonesia is fragmentary because of the lack of wide-area insecticide resistance surveillance. We collected Ae. aegypti from 32 districts and regencies in 27 Indonesian provinces and used WHO bioassays to evaluate their resistance to deltamethrin, permethrin, bendiocarb, and pirimiphos-methyl. To determine the possible resistance mechanisms of Ae. aegypti, synergism tests were conducted using piperonyl butoxide (PBO) and S,S,S-tributylphosphorotrithioates (DEF). The Ae. aegypti from all locations exhibited various levels of resistance to pyrethroids. Their resistance ratio (RR50) to permethrin and deltamethrin ranged from 4.08× to 127× and from 4.37× to 72.20×, respectively. In contrast with the findings of other studies, most strains from the highly urbanized cities on the island of Java (i.e., Banten, Jakarta, Bandung, Semarang, Yogyakarta, and Surabaya) exhibited low to moderate resistance to pyrethroids. By contrast, the strains collected from the less populated Kalimantan region exhibited very high resistance to pyrethroids. The possible reasons are discussed herein. Low levels of resistance to bendiocarb (RR50, 1.24-6.46×) and pirimiphos-methyl (RR50, 1.01-2.70×) were observed in all tested strains, regardless of locality. PBO and DEF synergists significantly increased the susceptibility of Ae. aegypti to permethrin and deltamethrin and reduced their resistance ratio to less than 16×. The synergism tests suggested the major involvement of cytochrome P450 monooxygenases and esterases in conferring pyrethroid resistance. On the basis of our results, we proposed a 6-month rotation of insecticides (deltamethrin + synergists ➝ bendiocarb ➝ permethrin + synergists ➝ pirimiphos-methyl) and the use of an insecticide mixture containing pyrethroid and pyrimiphos-methyl to control Ae. aegypti populations and overcome the challenge of widespread Ae. aegypti resistance to pyrethroid in Indonesia. |
format |
Article in Journal/Newspaper |
author |
Christina Natalina Silalahi Wu-Chun Tu Niann-Tai Chang G Veera Singham Intan Ahmad Kok-Boon Neoh |
author_facet |
Christina Natalina Silalahi Wu-Chun Tu Niann-Tai Chang G Veera Singham Intan Ahmad Kok-Boon Neoh |
author_sort |
Christina Natalina Silalahi |
title |
Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
title_short |
Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
title_full |
Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
title_fullStr |
Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
title_full_unstemmed |
Insecticide Resistance Profiles and Synergism of Field Aedes aegypti from Indonesia. |
title_sort |
insecticide resistance profiles and synergism of field aedes aegypti from indonesia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010501 https://doaj.org/article/a14e58984e834d28b30eefb2e4b4ccd1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010501 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010501 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010501 https://doaj.org/article/a14e58984e834d28b30eefb2e4b4ccd1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010501 |
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PLOS Neglected Tropical Diseases |
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16 |
container_issue |
6 |
container_start_page |
e0010501 |
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1766342919963279360 |