Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule )
Symptoms of a new vascular wilt were observed on several plants of Papaver nudicaule cultivated at the Regional Institute for Floriculture in Sanremo and in a nursery near Ventimiglia, Liguria region, Northern Italy. Morphology and ITS sequence analysis characterize the pathogen as Fusarium oxysporu...
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Firenze University Press
2015
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ftdoajarticles:oai:doaj.org/article:a0f97b459b7a494ab395a88d6a9e78b5 2023-05-15T16:50:07+02:00 Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) Giuseppe ORTU Domenico BERTETTI Patrizia MARTINI Maria Lodovica GULLINO Angelo GARIBALDI 2015-04-01T00:00:00Z https://doi.org/10.14601/Phytopathol_Mediterr-14303 https://doaj.org/article/a0f97b459b7a494ab395a88d6a9e78b5 EN eng Firenze University Press https://oajournals.fupress.net/index.php/pm/article/view/5603 https://doaj.org/toc/0031-9465 https://doaj.org/toc/1593-2095 doi:10.14601/Phytopathol_Mediterr-14303 0031-9465 1593-2095 https://doaj.org/article/a0f97b459b7a494ab395a88d6a9e78b5 Phytopathologia Mediterranea, Vol 54, Iss 1 (2015) Fusarium EF-1α endopolygalacturonase Botany QK1-989 article 2015 ftdoajarticles https://doi.org/10.14601/Phytopathol_Mediterr-14303 2022-12-30T21:39:58Z Symptoms of a new vascular wilt were observed on several plants of Papaver nudicaule cultivated at the Regional Institute for Floriculture in Sanremo and in a nursery near Ventimiglia, Liguria region, Northern Italy. Morphology and ITS sequence analysis characterize the pathogen as Fusarium oxysporum. To identify the forma specialis, eight isolates obtained from infected tissues were used for a phylogenetic analysis based on translation elongation factor 1-α (EF-1α) gene and four polygalacturonase genes (Pg1, Pg5, Pgx1 and Pgx4). After amplification by PCR, the gene sequences were aligned with corresponding sequences from other formae speciales of Fusarium oxysporum obtained from GenBank databases in order to build phylogenetic trees. Results obtained for each genomic region showed that the isolates derived from P. nudicaule form a unique group well separated from other formae speciales. To our knowledge, this is the first report of F. oxysporum on P. nudicaule and together with the phylogenetic analysis and positive pathogenicity tests permits us to introduce a new forma specialis named F. oxysporum f. sp. papaveris. Article in Journal/Newspaper Iceland Directory of Open Access Journals: DOAJ Articles Ventimiglia ENVELOPE(-61.483,-61.483,-64.433,-64.433) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Fusarium EF-1α endopolygalacturonase Botany QK1-989 |
spellingShingle |
Fusarium EF-1α endopolygalacturonase Botany QK1-989 Giuseppe ORTU Domenico BERTETTI Patrizia MARTINI Maria Lodovica GULLINO Angelo GARIBALDI Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
topic_facet |
Fusarium EF-1α endopolygalacturonase Botany QK1-989 |
description |
Symptoms of a new vascular wilt were observed on several plants of Papaver nudicaule cultivated at the Regional Institute for Floriculture in Sanremo and in a nursery near Ventimiglia, Liguria region, Northern Italy. Morphology and ITS sequence analysis characterize the pathogen as Fusarium oxysporum. To identify the forma specialis, eight isolates obtained from infected tissues were used for a phylogenetic analysis based on translation elongation factor 1-α (EF-1α) gene and four polygalacturonase genes (Pg1, Pg5, Pgx1 and Pgx4). After amplification by PCR, the gene sequences were aligned with corresponding sequences from other formae speciales of Fusarium oxysporum obtained from GenBank databases in order to build phylogenetic trees. Results obtained for each genomic region showed that the isolates derived from P. nudicaule form a unique group well separated from other formae speciales. To our knowledge, this is the first report of F. oxysporum on P. nudicaule and together with the phylogenetic analysis and positive pathogenicity tests permits us to introduce a new forma specialis named F. oxysporum f. sp. papaveris. |
format |
Article in Journal/Newspaper |
author |
Giuseppe ORTU Domenico BERTETTI Patrizia MARTINI Maria Lodovica GULLINO Angelo GARIBALDI |
author_facet |
Giuseppe ORTU Domenico BERTETTI Patrizia MARTINI Maria Lodovica GULLINO Angelo GARIBALDI |
author_sort |
Giuseppe ORTU |
title |
Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
title_short |
Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
title_full |
Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
title_fullStr |
Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
title_full_unstemmed |
Fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( Papaver nudicaule ) |
title_sort |
fusarium oxysporum f. sp. papaveris : a new forma specialis isolated from iceland poppy ( papaver nudicaule ) |
publisher |
Firenze University Press |
publishDate |
2015 |
url |
https://doi.org/10.14601/Phytopathol_Mediterr-14303 https://doaj.org/article/a0f97b459b7a494ab395a88d6a9e78b5 |
long_lat |
ENVELOPE(-61.483,-61.483,-64.433,-64.433) |
geographic |
Ventimiglia |
geographic_facet |
Ventimiglia |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Phytopathologia Mediterranea, Vol 54, Iss 1 (2015) |
op_relation |
https://oajournals.fupress.net/index.php/pm/article/view/5603 https://doaj.org/toc/0031-9465 https://doaj.org/toc/1593-2095 doi:10.14601/Phytopathol_Mediterr-14303 0031-9465 1593-2095 https://doaj.org/article/a0f97b459b7a494ab395a88d6a9e78b5 |
op_doi |
https://doi.org/10.14601/Phytopathol_Mediterr-14303 |
_version_ |
1766040296421851136 |