Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot
A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-base...
Published in: | Biology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3390/biology12050650 https://doaj.org/article/a0e0050bc54a42908d7f9bf5235e62f6 |
id |
ftdoajarticles:oai:doaj.org/article:a0e0050bc54a42908d7f9bf5235e62f6 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:a0e0050bc54a42908d7f9bf5235e62f6 2023-10-09T21:55:50+02:00 Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang 2023-04-01T00:00:00Z https://doi.org/10.3390/biology12050650 https://doaj.org/article/a0e0050bc54a42908d7f9bf5235e62f6 EN eng MDPI AG https://www.mdpi.com/2079-7737/12/5/650 https://doaj.org/toc/2079-7737 doi:10.3390/biology12050650 2079-7737 https://doaj.org/article/a0e0050bc54a42908d7f9bf5235e62f6 Biology, Vol 12, Iss 650, p 650 (2023) dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health Scophthalmus maximus Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3390/biology12050650 2023-09-10T00:41:36Z A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-based diet (FO group); (2) weekly alternate feeding between soybean oil (SO)- and FO-based diets (SO/FO group); and (3) weekly alternate feeding between beef tallow (BT)- and FO-based diets (BT/FO group). An intestinal bacterial community analysis showed that alternate feeding reshaped the intestinal microbial composition. Higher species richness and diversity of the intestinal microbiota were observed in the alternate-feeding groups. A PCoA analysis showed that the samples clustered separately according to the feeding strategy, and among the three groups, the SO/FO group clustered relatively closer to the BT/FO group. The alternate feeding significantly decreased the abundance of Mycoplasma and selectively enriched specific microorganisms, including short-chain fatty acid (SCFA)-producing bacteria, digestive bacteria ( Corynebacterium and Sphingomonas ), and several potential pathogens ( Desulfovibrio and Mycobacterium ). Alternate feeding may maintain the intestinal microbiota balance by improving the connectivity of the ecological network and increasing the competitive interactions within the ecological network. The alternate feeding significantly upregulated the KEGG pathways of fatty acid and lipid metabolism, glycan biosynthesis, and amino acid metabolism in the intestinal microbiota. Meanwhile, the upregulation of the KEGG pathway of lipopolysaccharide biosynthesis indicates a potential risk for intestinal health. In conclusion, short-term alternate feeding between dietary lipid sources reshapes the intestinal microecology of the juvenile turbot, possibly resulting in both positive and negative effects. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Biology 12 5 650 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health Scophthalmus maximus Biology (General) QH301-705.5 |
spellingShingle |
dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health Scophthalmus maximus Biology (General) QH301-705.5 Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
topic_facet |
dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health Scophthalmus maximus Biology (General) QH301-705.5 |
description |
A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-based diet (FO group); (2) weekly alternate feeding between soybean oil (SO)- and FO-based diets (SO/FO group); and (3) weekly alternate feeding between beef tallow (BT)- and FO-based diets (BT/FO group). An intestinal bacterial community analysis showed that alternate feeding reshaped the intestinal microbial composition. Higher species richness and diversity of the intestinal microbiota were observed in the alternate-feeding groups. A PCoA analysis showed that the samples clustered separately according to the feeding strategy, and among the three groups, the SO/FO group clustered relatively closer to the BT/FO group. The alternate feeding significantly decreased the abundance of Mycoplasma and selectively enriched specific microorganisms, including short-chain fatty acid (SCFA)-producing bacteria, digestive bacteria ( Corynebacterium and Sphingomonas ), and several potential pathogens ( Desulfovibrio and Mycobacterium ). Alternate feeding may maintain the intestinal microbiota balance by improving the connectivity of the ecological network and increasing the competitive interactions within the ecological network. The alternate feeding significantly upregulated the KEGG pathways of fatty acid and lipid metabolism, glycan biosynthesis, and amino acid metabolism in the intestinal microbiota. Meanwhile, the upregulation of the KEGG pathway of lipopolysaccharide biosynthesis indicates a potential risk for intestinal health. In conclusion, short-term alternate feeding between dietary lipid sources reshapes the intestinal microecology of the juvenile turbot, possibly resulting in both positive and negative effects. |
format |
Article in Journal/Newspaper |
author |
Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang |
author_facet |
Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang |
author_sort |
Xiuhua Ma |
title |
Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
title_short |
Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
title_full |
Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
title_fullStr |
Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
title_full_unstemmed |
Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
title_sort |
short-term alternate feeding between terrestrially sourced oil- and fish oil-based diets modulates the intestinal microecology of juvenile turbot |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/biology12050650 https://doaj.org/article/a0e0050bc54a42908d7f9bf5235e62f6 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Biology, Vol 12, Iss 650, p 650 (2023) |
op_relation |
https://www.mdpi.com/2079-7737/12/5/650 https://doaj.org/toc/2079-7737 doi:10.3390/biology12050650 2079-7737 https://doaj.org/article/a0e0050bc54a42908d7f9bf5235e62f6 |
op_doi |
https://doi.org/10.3390/biology12050650 |
container_title |
Biology |
container_volume |
12 |
container_issue |
5 |
container_start_page |
650 |
_version_ |
1779320008973746176 |