MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach
Abstract Background: Scorpions are widely known for the neurotoxic effects of their venoms, which contain peptides affecting ionic channels. Although Colombia is recognized for its scorpion diversity, only a few studies are available describing the venom content. Methods: In this descriptive study,...
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ftdoajarticles:oai:doaj.org/article:a0a1357d13e04c3aa42ed847fdf714d5 2023-05-15T15:16:07+02:00 MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach Sebastian Estrada-Gómez Leidy Johana Vargas-Muñoz Monica Maria Saldarriaga-Córdoba Arie van der Meijden 2021-07-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0173 https://doaj.org/article/a0a1357d13e04c3aa42ed847fdf714d5 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100316&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0173 https://doaj.org/article/a0a1357d13e04c3aa42ed847fdf714d5 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) Scorpion Venom Colombia MS analysis Toxins Sodium channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2021 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0173 2022-12-31T08:02:00Z Abstract Background: Scorpions are widely known for the neurotoxic effects of their venoms, which contain peptides affecting ionic channels. Although Colombia is recognized for its scorpion diversity, only a few studies are available describing the venom content. Methods: In this descriptive study, we analyzed the MS/MS sequence, electrophoretic and chromatographic profile linked to a bioinformatics analysis of the scorpions Chactas reticulatus (Chactidae), Opisthacanthus elatus (Hormuridae), Centruroides edwardsii (Buthidae) and Tityus asthenes (Buthidae) from Colombia. Results: Each scorpion showed a specific electrophoretic and chromatographic profile. The electrophoretic profiles indicate the presence of high molecular mass compounds in all venoms, with a predominance of low molecular mass compounds in the Buthidae species. Chromatographic profiles showed a similar pattern as the electrophoretic profiles. From the MS/MS analysis of the chromatographic collected fractions, we obtained internal peptide sequences corresponding to proteins reported in scorpions from the respective family of the analyzed samples. Some of these proteins correspond to neurotoxins affecting ionic channels, antimicrobial peptides and metalloproteinase-like fragments. In the venom of Tityus asthenes, the MSn analysis allowed the detection of two toxins affecting sodium channels covering 50% and 84% of the sequence respectively, showing 100% sequence similarity. Two sequences from Tityus asthenes showed sequence similarity with a phospholipase from Opisthacanthus cayaporum indicating the presence of this type of toxin in this species for the first time. One sequence matching a hypothetical secreted protein from Hottentotta judaicus was found in three of the studied venoms. We found that this protein is common in the Buthidae family whereas it has been reported in other families - such as Scorpionidae - and may be part of the evolutionary puzzle of venoms in these arachnids. Conclusion: Buthidae venoms from Colombia can be considered an ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 27 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Scorpion Venom Colombia MS analysis Toxins Sodium channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Scorpion Venom Colombia MS analysis Toxins Sodium channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Sebastian Estrada-Gómez Leidy Johana Vargas-Muñoz Monica Maria Saldarriaga-Córdoba Arie van der Meijden MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
topic_facet |
Scorpion Venom Colombia MS analysis Toxins Sodium channels Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Scorpions are widely known for the neurotoxic effects of their venoms, which contain peptides affecting ionic channels. Although Colombia is recognized for its scorpion diversity, only a few studies are available describing the venom content. Methods: In this descriptive study, we analyzed the MS/MS sequence, electrophoretic and chromatographic profile linked to a bioinformatics analysis of the scorpions Chactas reticulatus (Chactidae), Opisthacanthus elatus (Hormuridae), Centruroides edwardsii (Buthidae) and Tityus asthenes (Buthidae) from Colombia. Results: Each scorpion showed a specific electrophoretic and chromatographic profile. The electrophoretic profiles indicate the presence of high molecular mass compounds in all venoms, with a predominance of low molecular mass compounds in the Buthidae species. Chromatographic profiles showed a similar pattern as the electrophoretic profiles. From the MS/MS analysis of the chromatographic collected fractions, we obtained internal peptide sequences corresponding to proteins reported in scorpions from the respective family of the analyzed samples. Some of these proteins correspond to neurotoxins affecting ionic channels, antimicrobial peptides and metalloproteinase-like fragments. In the venom of Tityus asthenes, the MSn analysis allowed the detection of two toxins affecting sodium channels covering 50% and 84% of the sequence respectively, showing 100% sequence similarity. Two sequences from Tityus asthenes showed sequence similarity with a phospholipase from Opisthacanthus cayaporum indicating the presence of this type of toxin in this species for the first time. One sequence matching a hypothetical secreted protein from Hottentotta judaicus was found in three of the studied venoms. We found that this protein is common in the Buthidae family whereas it has been reported in other families - such as Scorpionidae - and may be part of the evolutionary puzzle of venoms in these arachnids. Conclusion: Buthidae venoms from Colombia can be considered an ... |
format |
Article in Journal/Newspaper |
author |
Sebastian Estrada-Gómez Leidy Johana Vargas-Muñoz Monica Maria Saldarriaga-Córdoba Arie van der Meijden |
author_facet |
Sebastian Estrada-Gómez Leidy Johana Vargas-Muñoz Monica Maria Saldarriaga-Córdoba Arie van der Meijden |
author_sort |
Sebastian Estrada-Gómez |
title |
MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
title_short |
MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
title_full |
MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
title_fullStr |
MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
title_full_unstemmed |
MS/MS analysis of four scorpion venoms from Colombia: a descriptive approach |
title_sort |
ms/ms analysis of four scorpion venoms from colombia: a descriptive approach |
publisher |
SciELO |
publishDate |
2021 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0173 https://doaj.org/article/a0a1357d13e04c3aa42ed847fdf714d5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100316&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0173 https://doaj.org/article/a0a1357d13e04c3aa42ed847fdf714d5 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0173 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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27 |
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1766346424077778944 |