Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax
Abstract Background The knowledge on population structure of the parasite isolates has contributed greatly to understanding the dynamics of the disease transmission for designing and evaluating malaria vaccines as well as for drug applications. msp-1 and msp-3α genes have been used as a genetic mark...
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ftdoajarticles:oai:doaj.org/article:a04c70e1fb1a4cc4becec956280e503d 2023-05-15T15:16:11+02:00 Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax Zakeri Sedigheh Barjesteh Hesam Djadid Navid D 2006-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-5-53 https://doaj.org/article/a04c70e1fb1a4cc4becec956280e503d EN eng BMC http://www.malariajournal.com/content/5/1/53 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-53 1475-2875 https://doaj.org/article/a04c70e1fb1a4cc4becec956280e503d Malaria Journal, Vol 5, Iss 1, p 53 (2006) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2006 ftdoajarticles https://doi.org/10.1186/1475-2875-5-53 2022-12-31T08:32:57Z Abstract Background The knowledge on population structure of the parasite isolates has contributed greatly to understanding the dynamics of the disease transmission for designing and evaluating malaria vaccines as well as for drug applications. msp-1 and msp-3α genes have been used as a genetic marker in population studies of Plasmodium vivax isolates. In this study, msp-3α was compared and assessed with msp-1 marker in order to find whether msp-3α is a reliable genetic marker for P. vivax population studies. Methods This comparative study was designed and carried out as the first assessment of diversity in Pvmsp-3α gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the 50 northern and 94 southern P. vivax isolates from Iran, which had been analysed before for msp-1 gene. Results Three allele size as, Type A (1.8 kb), Type B (1.5 kb) and Type C (1.2 kb) have been detected among both northern and southern isolates based on PCR results. Type C (70%) and Type A (68.7%) were the predominant fragments among northern and southern parasites, respectively. 99 distinct Pvmsp -3α fragments defined by the size were detected in the 94 southern samples by PCR analysis. However, no mixed genotype infections have been detected among northern isolates. Based on restriction pattern from digestion with Hha I and Alu I 12 and 49 distinct allelic variants have been detected among 50 northern and 94 southern isolates. However, based on msp-1 gene, 30 distinct variants identified in all 146-sequenced Iranian P. vivax isolate. Conclusion The results suggested that PCR-RFLP on msp-3α gene is an adequate, applicable and easily used technique for molecular epidemiology studies of P. vivax isolates without the need for further sequencing analysis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 5 1 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Zakeri Sedigheh Barjesteh Hesam Djadid Navid D Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The knowledge on population structure of the parasite isolates has contributed greatly to understanding the dynamics of the disease transmission for designing and evaluating malaria vaccines as well as for drug applications. msp-1 and msp-3α genes have been used as a genetic marker in population studies of Plasmodium vivax isolates. In this study, msp-3α was compared and assessed with msp-1 marker in order to find whether msp-3α is a reliable genetic marker for P. vivax population studies. Methods This comparative study was designed and carried out as the first assessment of diversity in Pvmsp-3α gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the 50 northern and 94 southern P. vivax isolates from Iran, which had been analysed before for msp-1 gene. Results Three allele size as, Type A (1.8 kb), Type B (1.5 kb) and Type C (1.2 kb) have been detected among both northern and southern isolates based on PCR results. Type C (70%) and Type A (68.7%) were the predominant fragments among northern and southern parasites, respectively. 99 distinct Pvmsp -3α fragments defined by the size were detected in the 94 southern samples by PCR analysis. However, no mixed genotype infections have been detected among northern isolates. Based on restriction pattern from digestion with Hha I and Alu I 12 and 49 distinct allelic variants have been detected among 50 northern and 94 southern isolates. However, based on msp-1 gene, 30 distinct variants identified in all 146-sequenced Iranian P. vivax isolate. Conclusion The results suggested that PCR-RFLP on msp-3α gene is an adequate, applicable and easily used technique for molecular epidemiology studies of P. vivax isolates without the need for further sequencing analysis. |
format |
Article in Journal/Newspaper |
author |
Zakeri Sedigheh Barjesteh Hesam Djadid Navid D |
author_facet |
Zakeri Sedigheh Barjesteh Hesam Djadid Navid D |
author_sort |
Zakeri Sedigheh |
title |
Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
title_short |
Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
title_full |
Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
title_fullStr |
Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
title_full_unstemmed |
Merozoite surface protein-3α is a reliable marker for population genetic analysis of Plasmodium vivax |
title_sort |
merozoite surface protein-3α is a reliable marker for population genetic analysis of plasmodium vivax |
publisher |
BMC |
publishDate |
2006 |
url |
https://doi.org/10.1186/1475-2875-5-53 https://doaj.org/article/a04c70e1fb1a4cc4becec956280e503d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 5, Iss 1, p 53 (2006) |
op_relation |
http://www.malariajournal.com/content/5/1/53 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-5-53 1475-2875 https://doaj.org/article/a04c70e1fb1a4cc4becec956280e503d |
op_doi |
https://doi.org/10.1186/1475-2875-5-53 |
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Malaria Journal |
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5 |
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1766346471569883136 |