Isolation of Efficient Metal-Binding Bacteria from Boreal Peat Soils and Development of Microbial Biosorbents for Improved Nickel Scavenging

• Published in: Water
• Main Authors: Hanna Virpiranta, Michal Banasik, Sanna Taskila, Tiina Leiviskä, Maiju Halttu, Ville-Hermanni Sotaniemi, Juha Tanskanen
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2020
• Subjects: metal-binding bacteria; siderophores; soil bacteria; Actinobacteria; Microbacterium sp.; biosorption; nickel; Hydraulic engineering; TC1-978; Water supply for domestic and industrial purposes; TD201-500; Northern Finland
• Online Access: https://doi.org/10.3390/w12072000; https://doaj.org/article/a034cd8f5d6a4c0dae4dc6071a3c6a03

Boreal peatlands with low iron availability are a potential, but rarely studied, source for the isolation of bacteria for applications in metal sorption. The present research focused on the isolation and identification of Actinobacteria from northern Finland, which can produce siderophores for metal capture. The 16S rDNA analysis showed that isolated strains belonged to Firmicutes ( Bacillus sp.) and Actinobacteria ( Microbacterium sp.). The culture most efficiently producing siderophores in the widest array of the media was identified as Microbacterium sp. The most appropriate media for siderophore production by the Microbacterium strain were those prepared with glucose supplemented with asparagine or glutamic acid, and those prepared with glycerol or fructose supplemented with glutamic acid. The microorganism obtained and its siderophores were used to develop Sphagnum moss-based hybrid biosorbents. It was showed that the hybrid sorbent could bind nickel ions and that the nickel removal was enhanced by the presence of siderophores. Bacterial cells did not have a significant effect on sorption efficiency compared to the use of siderophores alone. The microbial biosorbent could be applied in the final effluent treatment stage for wastewater with low metal concentrations.