Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a...
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ftdoajarticles:oai:doaj.org/article:a02f4a0c92e341709806d5766b8fa3f0 2023-05-15T15:12:25+02:00 Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit NH Tan G Ponnudurai SY Fung 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000200014 https://doaj.org/article/a02f4a0c92e341709806d5766b8fa3f0 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200014 1678-9199 https://doaj.org/article/a02f4a0c92e341709806d5766b8fa3f0 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 340-346 (2009) Calloselasma rhodostoma venom thrombin-like enzyme hemorrhagin serum kinetics rabbit ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000200014 2023-01-08T01:27:50Z The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme and rhodostoxin remained at relatively high levels 72 hours after administration. Data suggest that various venom components bind to tissue at the injection site with different affinities and that conjugated venom components were continuously released into circulation at different rates. The prolonged high serum levels of both thrombin-like enzyme and hemorrhagin are consistent with the clinical picture of prolonged clotting deficiency in severe cases of C. rhodostoma envenomation. Our results also suggest that since venom components are being released into and eliminated from the circulation at different rates, the "average composition" of the venom antigen in the circulation changes over time. This implies that data from ELISA quantification of antigen levels from serum venom employing "whole venom" as reagent must be interpreted with care. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 15 2 |
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topic |
Calloselasma rhodostoma venom thrombin-like enzyme hemorrhagin serum kinetics rabbit ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
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Calloselasma rhodostoma venom thrombin-like enzyme hemorrhagin serum kinetics rabbit ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 NH Tan G Ponnudurai SY Fung Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
topic_facet |
Calloselasma rhodostoma venom thrombin-like enzyme hemorrhagin serum kinetics rabbit ELISA Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom - specifically two of its components, the major hemorrhagin (rhodostoxin) and a thrombin-like enzyme - was examined in a rabbit by double-sandwich enzyme-linked immunosorbent assay (ELISA). The animal received intramuscularly a 1.0-mg/kg dose of C. rhodostoma venom. The venom level in serum peaked 12 hours after the injection, followed by a gradual decline and finally reached low rates 72 hours after administration. The serum kinetic profile of venom components, however, did not correspond to the profile of the whole C. rhodostoma venom. The serum levels of the C. rhodostoma thrombin-like enzyme increased slowly and peaked only 48 hours post-injection. Then both thrombin-like enzyme and rhodostoxin remained at relatively high levels 72 hours after administration. Data suggest that various venom components bind to tissue at the injection site with different affinities and that conjugated venom components were continuously released into circulation at different rates. The prolonged high serum levels of both thrombin-like enzyme and hemorrhagin are consistent with the clinical picture of prolonged clotting deficiency in severe cases of C. rhodostoma envenomation. Our results also suggest that since venom components are being released into and eliminated from the circulation at different rates, the "average composition" of the venom antigen in the circulation changes over time. This implies that data from ELISA quantification of antigen levels from serum venom employing "whole venom" as reagent must be interpreted with care. |
format |
Article in Journal/Newspaper |
author |
NH Tan G Ponnudurai SY Fung |
author_facet |
NH Tan G Ponnudurai SY Fung |
author_sort |
NH Tan |
title |
Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
title_short |
Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
title_full |
Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
title_fullStr |
Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
title_full_unstemmed |
Serum kinetics of Calloselasma rhodostoma (Malayan pit viper) venom components in a rabbit |
title_sort |
serum kinetics of calloselasma rhodostoma (malayan pit viper) venom components in a rabbit |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000200014 https://doaj.org/article/a02f4a0c92e341709806d5766b8fa3f0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 340-346 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200014 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200014 1678-9199 https://doaj.org/article/a02f4a0c92e341709806d5766b8fa3f0 |
op_doi |
https://doi.org/10.1590/S1678-91992009000200014 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
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15 |
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